Specificity of Various Mitochondrial DNA (<i>mt</i>DNA), <i>ND5</i>, <i>D-Loop</i>, and <i>Cty-b</i> DNA Primers in Detecting Pig (<i>Sus scrofa</i>) DNA Fragments

Abstract

Polymerase Chain Reaction (PCR) is an accurate, simple and fast analytical method. This technique is widely used in the identification of meat adulteration and meat-based processed food products. Three Mitochondrial DNA (mt-DNA) primers NADH Dehydrogenase sub unit 5 ( ND5 ), D-Loop , and Cytochrome b ( Cyt-b ) were tested for their specificity in detecting of pig ( Sus scrofa ) DNA fragments. DNA genome from 6 meat samples (pork, beef, goat, lamb, and chicken) was amplified by PCR technique using three pairs of primers ( ND5, D-Loop , and Cyt-b ) and sequenced. The results of amplification using the three primers produced specific DNA bands with the lengths of 232 bp, 951 bp, and 404 bp, respectively. Comparison results with ND5, D-Loop, and Cyt-b gene sequences resulted in similarity values of 100%, 97%, and 99%, respectively. These showed that the mt-DNA primers of ND5, D-Loop , and Cyt-b genes can be recommended as specific primers in detecting pig ( Sus scrofa ) DNA fragments.