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Expression of Difficult-to-Express Proteins, Human IL-12 and IFN-<i>γ</i>

¹ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
² Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
³ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
⁴ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
⁵ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
⁶ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
⁷ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
⁸ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
⁹ Akita Konno Co., Ltd., 248 Aza Kariwano, Daisen-shi, Akita, Japan
¹⁰ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
¹¹ Department of Life Science, Graduate School and Faculty of Engineering Science, Akita University, Akita, Japan
¹² Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

American Journal of Molecular Biology·Volume 11 (2021)·Pages 29–37·Published 4 March 2021·DOI10.4236/ajmb.2021.112003

Abstract

It is known to be that lactic acid bacteria induce the IL-12. IL-12 activates NK cells and promotes the production of IFN- γ . IFN- γ activates macrophages, resulting in enhanced phagocytosis and bactericidal activity. We have been investigating fermented foods that activate the immune function. For that purpose, a specific antibody is required. We tried to express IL-12p35 by the usual method, but IL-12p35 was not expressed at all. In the present study, we constructed, purified human IL-12p35 and obtained a specific antibody against IL-12p35. We also purified human IFN- γ and obtained specific antibody against IFN- γ . We have established a method for expressing poorly expressed proteins. The method we have established can be applied to the purification of poorly expressed proteins and antibody production.

KeywordsDifficult-to-Express ProteinsIL-12IFN-&lti&gtγ&lt/i&gtPurificationAntibody Production

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Expression of Difficult-to-Express Proteins, Human IL-12 and IFN-<i>γ</i>

Abstract

References (5)