Diversity and Distribution of Staphylococcal Chromosomal Cassettes Mec (SCCmec) Types I, II and III in Coagulase-Negative Staphylococcal Strains Isolated from Surfaces and Medico-Technical Materials of the University Hospital of Abomey-Calavi/Sô-Ava — Oak Academic Publishing
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Diversity and Distribution of Staphylococcal Chromosomal Cassettes Mec (SCCmec) Types I, II and III in Coagulase-Negative Staphylococcal Strains Isolated from Surfaces and Medico-Technical Materials of the University Hospital of Abomey-Calavi/Sô-Ava
Laboratoire de Biologie Moléculaire et de Bio-Informatique Appliquée à la Génomique, Ecole Nationale Supérieure de Bioscience et de Biotechnologies Appliquées, Université Nationale des Sciences, Technologies, Ingénierie et Mathématique, Dassa-Zoumè, Bénin
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Laboratoire de Biochimie, Biologie Moléculaire et Environnement, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Biologie Moléculaire et de Bio-Informatique Appliquée à la Génomique, Ecole Nationale Supérieure de Bioscience et de Biotechnologies Appliquées, Université Nationale des Sciences, Technologies, Ingénierie et Mathématique, Dassa-Zoumè, Bénin
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Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Génétique Moléculaire et d’Analyse des Génomes, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
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Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
1 Laboratoire de Biologie Moléculaire et de Bio-Informatique Appliquée à la Génomique, Ecole Nationale Supérieure de Bioscience et de Biotechnologies Appliquées, Université Nationale des Sciences, Technologies, Ingénierie et Mathématique, Dassa-Zoumè, Bénin
2 Laboratoire de Biochimie, Biologie Moléculaire et Environnement, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
3 Laboratoire de Biologie Moléculaire et de Bio-Informatique Appliquée à la Génomique, Ecole Nationale Supérieure de Bioscience et de Biotechnologies Appliquées, Université Nationale des Sciences, Technologies, Ingénierie et Mathématique, Dassa-Zoumè, Bénin
4 Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
5 Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
6 Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
7 Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
8 Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
9 Laboratoire de Génétique Moléculaire et d’Analyse des Génomes, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
10 Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Abomey-Calavi, Bénin
The coagulase-negative staphylococci (CoNS) have long been considered to be low pathogenicity. The possibility of a horizontal transfer of resistance and virulence genes from S. aureus to CoNS could increase the pathogenicity of these bacteria. The objective of this work is to contribute to a better knowledge of the pathogenicity of (CoNS) strains isolated from surfaces and medico-technical materials of the University Hospital of Abomey-Calavi/S ô -Ava. Seventy strains of CoNS isolated from surfaces and medico-technical materials of the University Hospital of Abomey-Calavi were tested for methicillin resistance. The resistance to methicillin was evaluated phenotypically by the resistance of the strains to cefoxitin and then confirmed by the search for the mecA gene using PCR. The genes encoding staphylococcal chromosomal cassette (SCCmec) types I, II and III originally found in S. aureus were tested in CoNS by multiplex PCR using specific primers. All the strains studied showed resistance to methicillin. However, only 28.5% (20/70) carried the mecA gene. SCCmec was identified in only 17.14% (12/70) of these strains. Four strains carried mecA gene as well as one of the three types of SCCmec searched. SCCmec types I, II and III were identified in CoNS strains studied. SCCmec type I was the most frequent chromosomal cassette in mecA + strains, only or in association with another SCCmec. The study also revealed methicillin-resistant strains carrying SCCmec lacking the mecA gene. Finally, 60% (12/20) of the strains were found to be non-typeable. Our results show that CoNS strains present a high resistance to methicillin and the source of this resistance in the CoNS of our study is not only the mecA gene. There is also a high diversity of SCCmec, justified by a large number of non-typeable CoNS strains. The mecA − SCCmec + methicillin-resistant strains deserve to be sequenced for further studies.
Valencia-Rey, P., Weinberg, J., Miller, N.S. and Barlam, T.F. (2015) Coagulase-Negative Staphylococcal Bloods Tream Infections: Does Vancomycin Remain Appropriate Empirictherapy? Journal of Infection, 71, 53-60. https://doi.org/10.1016/j.jinf.2015.02.007
Lowy, F.D. (1998) Staphylococcus aureus Infections. New England Journal of Medicine, 339, 520-532. https://doi.org/10.1056/NEJM199808203390806
Bush, L.M. (2019) Le Manuel MSD Version pour le grand public. https://www.msdmanuals.com
Malachowa, N. and Deleo, F.R. (2010) Mobile Genetic Elements of Staphylococcus aureus. Cellular and Molecular Life Sciences, 67, 3057-3071. https://doi.org/10.1007/s00018-010-0389-4
Besnier, J.M., Bastides, F. and Choutet, P. (1997) Thérapeutique des infections à Staphylococcus aureus sensible à la méticilline (SAMS). Médecine et Maladies Infectieuses, 27, 225-240. https://doi.org/10.1016/S0399-077X(97)80024-8
Drawz, S.M. and Bonomo, R.A. (2010) Three Decades of bêta-lactamase Inhibitors. Clinical Microbiology Reviews, 23, 160-201. https://doi.org/10.1128/CMR.00037-09
Wolff, K.A., Sherman, M. and Nguyen, L. (2011) Chapter 8. Potentiation of Available Antibiotics by Targeting Resistance—An Emerging Trend in Tuberculosis Drug Development. In: Rundfeldt, C., Ed., Drug Development—A Case Study-Based Insight into Modern Strategies, Intech Publisher Inc., London, 183-208.
Chambers, H.F. and Deleo, F.R. (2009) Waves of Resistance: Staphylococcus aureus in the Antibiotic Era. Nature Reviews. Microbiology, 7, 629. https://doi.org/10.1038/nrmicro2200
García-álvarez, L., Holden, M.T., Lindsay, H., Webb, C.R., Brown, D.F. and Curran, M.D. (2011) Meticillin-Resistant Staphylococcus aureus with a Novel mecA Homologue in Human and Bovine Populations in the UK and Denmark: A Descriptive Study. The Lancet Infectious Diseases, 8, 595-603. https://doi.org/10.1016/S1473-3099(11)70126-8
Katayama, Y., Takeuchi, F., Ito, T., Ma, X.X., Ui-Mizutani, Y., Kobayashi, I. and Hiramastu, K. (2003) Identification in Methicillin-Susceptible Staphylococcus hominis of an Active Primordial Mobile Genetic Element for the Staphylococcal Cassette Chromosome mec of Methicillin-Resistant Staphylococcus aureus. Journal Bacteriology, 185, 2711-2722. https://doi.org/10.1128/JB.185.9.2711-2722.2003
Ganga, R., Riederer, K., Sharma, M., Fakih, M.G., Johnson, L.B., Shemes, S. and Khatib, R. (2009) Role of SCCmec Typein Outcome of Staphylococcus aureus Bacteremia in a Single Medical Center. Journal of Clinical Microbiology, 47, 590-595. https://doi.org/10.1128/JCM.00397-08
Socohou, A., Sina, H., Degbey, C., Nanoukon, C., Chabi Sika, K., Ahouandjinou, H., Lehmane, H., Baba-Moussa, F. and Baba-Moussa, L. (2020) Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains from Isolated Surfaces and Medicotechnical Materials. International Journal of Microbiology, 2020, Article ID: 6512106. https://doi.org/10.1155/2020/6512106
European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2019) Recommendations, European Committee on Antimicrobial Susceptibility Testing, Varjo, Sweden.
Becker, K., Heilmann, C. and Peters, G. (2014) Coagulase-Negative Staphylococci. Clinical Microbiology Reviews, 27, 870-926. https://doi.org/10.1128/CMR.00109-13
Rakotozandrindrainy, N. (2018) Identification et caractérisation des Staphylocoques à Coagulase Négative résistants à la méthicilline isolés d’échantillons sanguins de patients fébriles dans les centres de santé de la région Analamanga-Antananarivo. Mémoire, Université D’Antananarivo, Madagascar.
Oliveira, A.D.D., d’Azevedo, P.A., De Sousa, L.B., Viana-Niero, C., Francisco, W., Lottenberg, C., Dalla, M., Martino, V. and Hofling-Lima, A.L. (2007) Laboratory Detection Methods for Methicillin Resistance in Coagulase Negative Staphylococcus Isolated from Ophthalmic Infections. The Arquivos Brasileiros Oftalmologia, 70, 667-675. https://doi.org/10.1590/S0004-27492007000400018
Asante, J., Hetsa, B.A., Amoako, D.G., Abia, A.L.K., Bester, L.A. and Essack, S.Y. (2021) Multidrug Resistant Coagulase-Negative Staphylococci Isolated from Bloodstream in the Umgungundlovu District of KwaZulu-Natal Province in South Africa: Emerging Pathogens. Antibiotics, 10, 198. https://doi.org/10.3390/antibiotics10020198
Seng, R., Leungtongkam, U., Thummeepak, R., Chatdumrong, W. and Sitthisak, S. (2017) High Prevalence of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from a University Environment in Thailand. International Microbiology, 20, 65-73.
Bhowmik, D., Dasa, B.J., Pandeya, P., Chetria, S., Chanda, D.D. and Bhattacharjeea, A. (2019) Anarray of Multiplex PCR Assays for Detection of Staphylococcal Chromosomal Cassette mec (SCCmec) Types among Staphylococcal Isolates. Journal of Microbiological Methods, 166, Article ID: 105733. https://doi.org/10.1016/j.mimet.2019.105733
Xu, Z., Shah, H.N., Misra, R., Chen, J., Zhang, W., Liu, Y., Cutler, R.R., Mkrtchyan, V.H., et al. (2018) The Prevalence, Antibiotic Resistance and mecA Characterization of Coagulase Negative Staphylococci Recovered from Non-Healthcare Settings in London, UK. Antimicrobial Resistance and Infection Control, 7, 73. https://doi.org/10.1186/s13756-018-0367-4
Chen, X., Li, W., Zheng, H., Du, H., Zhang, L., Zhang, L., Che, J., Wu, Y., Liu, S. and Lu, J. (2017) Extrême Diversity and Multiple SCCmec Elements in Coagulase-Negative Staphylococcus Found in the Clinic and Community in Beijing, China. Annals Clinical Microbiology Antimicrobials, 16, 57. https://doi.org/10.1186/s12941-017-0231-z
Hussain, Z., Stoakes, L., Lannigan, R., Longo, S. and Nancekivell, B. (1998) Evaluation of Screening and Commercial Methods for Detection of Methicillin Resistance in Coagulase-Negative Staphylococci. Journal of Clincali Microbiology, 36, 273-274. https://doi.org/10.1128/JCM.36.1.273-274.1998
Garza-Gonzalez, E., Morfin-Otero, R., Llaca-Diaz, J.M. and Rodriguez-Noriega, E. (2010) Staphylococcal Cassette Chromosome mec (SCCmec) in Methicillin-Resistant Coagulase-Negative Staphylococci. A Review and the Experience in a Tertiary-Care Setting. Epidemiology and Infection, 138, 644-654. https://doi.org/10.1017/S0950268809991361
Boisset, S. (2020) Résistances Aux Antibiotiques Chez Les Bactéries à Gram Positif. UFR Médecine-Université Grenoble Alpes, Grenoble.
Centre national de référence des Staphylocoques (2020) Rapport annuel d’activité CNR des Staphylocoques. Centre National de Référence, 28-30.
Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C. and Hiramatsu, K. (2001) Structural Comparison of Three Types of Staphylococcal Cassette Chromosome mec Integrated in the Chromosome in Methicillin-Resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 45, 1323-1336. https://doi.org/10.1128/AAC.45.5.1323-1336.2001
Hiramatsu, K., Katayama, Y., Yuzawa, H. and Ito, T. (2002) Molecular Genetics of Methicillin-Resistant Staphylococcus aureus. International Journal of Medical Microbiology, 292, 67-74. https://doi.org/10.1078/1438-4221-00192
Oliveira, D.C. and Tomasz, A.L.H. (2002) Secrets of Success of a Human Pathogen: Molecular Evolution of Pandemic Clones of Methicillin-Resistant Staphylococcus aureus. The Lancet Infectious Diseases, 2, 180-189. https://doi.org/10.1016/S1473-3099(02)00227-X
Xu, Z., Shi, L., Alam, M.J., Li, L. and Yamasaki, S. (2008) Integron-Bearing Methicillin-Resistant Coagulase-Negative Staphylococci in South China 2001-2004. FEMS Microbiology Letters, 278, 223-230. https://academic.oup.com/femsle/article/278/2/223/513107 https://doi.org/10.1111/j.1574-6968.2007.00994.x
Van Griethuysen, A., van Loo, I., Van Belkum, A., Vandenbroucke-Grauls, C., Wannet, W., van Keulen, P. and Kluytmans, J. (2005) Loss of the mecA Gene during Storage of Methicillin-Resistant Staphylococcus aureus (MRSA) Strains. Journal of Clinical Microbiology, 43, 1361-1365. https://doi.org/10.1128/JCM.43.3.1361-1365.2005
Olayinka, B.O., Olayinka, A.T., Obajuluwa, A.F., Onaolapo, J.A. and Olurinola, P.F. (2009) Absence of meca Gene in Methicillin-Resistant Staphylococcus aureus Isolates. African Journal of Infectious Diseases, 3, 49-56. https://doi.org/10.4314/ajid.v3i2.55081