Streptococcal Pyrogenic Exotoxin Genes SpeA and SpeB in Isolates of <i>Streptococcus pyogenes</i> from Children with Pharyngitis, Gezira State, Sudan
- 1 Department of Microbiology, Faculty of Science, University of Gezira, Wad Medani, Sudan
- 2 Department of Molecular and Immunogenetics, NCI, University of Gezira, Wad Medani, Sudan
- 3 Pediatric Cardiologist Faculty of Medicine, University of Gezira, Wad Medani, Sudan
- 4 Department of Medical Parasitology, Faculty of Medical Laboratory Science, University of Gezira, Wad Medani, Sudan
- 5 Department of Molecular and Immunogenetics, NCI, University of Gezira, Wad Medani, Sudan
Abstract
Background: Streptococcus pyogenes (group A streptococcus, GAS) is an important human bacterial pathogen. This organism possesses many virulence factors, Streptococcal pyrogenic exotoxinone of these. Aim: Detection of Streptococcal pyrogenic exotoxin SpeA and SpeB in isolated Streptococcus pyogenes. Methods: Tow hundred throat swab samples were collected from children with pharyngitis referred to Pediatric Teaching hospital and ENT hospital Wad medani, Sudan, from January to November 2021. The questionnaire was filled out to collect clinical and demographic data. Throat swabs were collected and processed with the standard microbiological procedure to isolate Streptococcus pyogenes . Antimicrobial susceptibility testing was done on all GAS isolates using the Kirby Bauer disk diffusion method according to clinical laboratory standard institute (CLSI) guidelines. Detection of Spy 1258 gene and Streptococcal pyrogenic exotoxins SpeA and SpeB were done by using Multiplex PCR. Results: Amongst the Tow hundred collected samples fifty-one isolates (25.5%) were identified as S. pyogenes . Antibiotic susceptibility testing showed that all the GAS isolates were sensitive to Azithromycin and Penicillin. Sensitivity to Erythromycin, Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%, respectively. SpeA was detected in 17 (33.3%) and SpeB in 48 (94.1%). Conclusion: Streptococcal pyrogenic exotoxin genes SpeA and SpeB were detected in 17 (33.3%) and 48 (94.1%) respectively of Streptococcus pyogenes isolates.
- Chuan, C.-N., and Wu, J.-J. (2008) Effects of Streptococcal Pyrogenic Exotoxin B on Pathogenesis of Streptococcus pyogenes. Journal of the Formosan Medical Association, 107, 677-685. https://doi.org/10.1016/S0929-6646(08)60112-6
- Friaes, A., Pinto, F.R., Silva-Costa, C., Ramirez, M. and Melo-Cristino, J. (2013) Superantigen Gene Complement of Streptococcus pyogenes—Relationship with Other Typing Methods and Short-Term Stability. European Journal of Clinical Microbiology & Infectious Diseases, 32, 115-125. https://doi.org/10.1007/s10096-012-1726-3
- Borek, A.L., Obszańska, K., Hryniewicz, W. and Sitkiewicz, I. (2012) Detection of Streptococcus pyogenes Virulence Factors by Multiplex PCR. Virulence, 3, 529-533. https://doi.org/10.4161/viru.21540
- Proft, T. and Fraser, J.D. (2016) Streptococcal Superantigens: Biological Properties and Potential Role in Disease. In: Ferretti, J.J., Stevens, D.L. and Fischetti, V.A., Eds., Streptococcus pyogenes: Basic Biology to Clinical Manifestations, University of Oklahoma Health Sciences Center, Oklahoma City.
- Helal, Z.M., Rizk, D.E., Adel El-Sokkary, M.M. and Hassan, R. (2020) Prevalence and Characterization of Streptococcus pyogenes Clinical Isolates from Different Hospitals and Clinics in Mansoura. International Journal of Microbiology, 2020, Article ID: 5814945. https://doi.org/10.1155/2020/5814945
- Chang, H.S., Shen, X.Z., Huang, G.Y., Fu, Z., Zheng, Y.J., Wang, L.B., Li, C.R., Liu, L., Shen, Y. and Liu, X.R. and Yang, Y.H. (2011) Molecular Analysis of Streptococcus pyogenes Strains Isolated from Chinese Children with Pharyngitis. Diagnostic Microbiology and Infectious Disease, 69, 117-122. https://doi.org/10.1016/j.diagmicrobio.2010.09.011
- Spaulding, A.R., Salgado-Pabón, W., Kohler, P.L., Horswill, A.R., Leung, D.Y. and Schlievert, P.M. (2013) Staphylococcal and Streptococcal Superantigen Exotoxins. Clinical Microbiology Reviews, 26, 422-447. https://doi.org/10.1128/CMR.00104-12
- Kalia, A. and Bessen, D.E. (2003) Presence of Streptococcal Pyrogenic Exotoxin A and C Genes in Human Isolates of Group G Streptococci. FEMS Microbiology Letters, 219, 291-295. https://doi.org/10.1016/S0378-1097(03)00022-3
- Wu, P.-C., Lo, W.-T., Chen, S.-J. and Wang, C.-C. (2014) Molecular Characterization of Group A Streptococcal Isolates Causing Scarlet Fever and Pharyngitis among Young Children: A Retrospective Study from a Northern Taiwan Medical Center. Journal of Microbiology, Immunology and Infection, 47, 304-310. https://doi.org/10.1016/j.jmii.2013.02.007