Cloning and Characterization of Eukaryotic Translation Initiation Factor 4E (eIF4E) Gene Family in <i>Ipomoea batatas</i> L. (Lam) for Understanding Hexaploid Sweetpotato-Virus Interactions — Oak Academic Publishing
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Cloning and Characterization of Eukaryotic Translation Initiation Factor 4E (eIF4E) Gene Family in <i>Ipomoea batatas</i> L. (Lam) for Understanding Hexaploid Sweetpotato-Virus Interactions
Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
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Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
,
Heliae Development, LLC, Gilbert, USA
,
Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
,
Integrative Biosciences PhD Program, Tuskegee University, Tuskegee, USA
,
Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
,
Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
,
Integrative Biosciences PhD Program, Tuskegee University, Tuskegee, USA
,
Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
1 Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
2 Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
3 Heliae Development, LLC, Gilbert, USA
4 Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
5 Integrative Biosciences PhD Program, Tuskegee University, Tuskegee, USA
6 Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
7 Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
8 Integrative Biosciences PhD Program, Tuskegee University, Tuskegee, USA
9 Plant Biotech and Genomics Research Lab, Department of Agriculture and Environmental Sciences, College of Agriculture, Environment and Nutrition Sciences (CAENS), Tuskegee University, Tuskegee, USA
Characterization of genes related to sweetpotato viral disease resistance is critical for understanding plant-pathogen interactions, especially with feathery mottle virus infection. For example, genes encoding eukaryotic translation initiation factor ( eIF )4 E , its isoforms, eIF ( iso )4 E , and the cap-binding protein ( CBP ) in plants, have been implicated in viral infections aside from their importance in protein synthesis. Full-length cDNA encoding these putative eIF targets from susceptible/resistant and unknown hexaploid sweetpotato ( Ipomoea batatas L. Lam) were amplified based on primers designed from the diploid wild-type relative Ipomoea trifida consensus sequences, and designated Ib eIF4E, Ib eIF(iso)4E and Ib CBP. Comparative analyses following direct-sequencing of PCR-amplified cDNAs versus the cloned cDNA sequences identified multiple homeoalleles: one to four IbeIF 4 E , two to three IbeIF ( iso )4 E , and two IbCBP within all cultivars tested. Open reading frames were in the length of 696 bp Ib eIF4E, 606 bp Ib eIF(iso)4E, and 675 bp Ib CBP. The encoded single polypeptide lengths were 232, 202, and 225 amino acids for Ib eIF4E, Ib eIF(iso)4E, and Ib CBP, with a calculated protein molecular mass of 26 kDa, 22.8 kDa, and 25.8 kDa, while their theoretical isoelectric points were 5.1, 5.57, and 6.6, respectively. Although the homeoalleles had similar sequence lengths, single nucleotide polymorphisms and multi-allelic variations were detected within the coding sequences. The multi-sequence alignment performed revealed a 66.9% - 96.7% sequence similarity between the predicted amino acid sequences obtained from the homeoalleles and closely related species. Furthermore, phylogenetic analysis revealed ancestral relationships between the eIF4E homeoalleles and other species. The outcome herein on the eIF4E superfamily and its correlation in sequence variations suggest opportunities to decipher the role of eIF4E in hexaploid sweetpotato feathery mottle virus infection.
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