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TurboID Proximity Labeling of a Protocadherin Protein to Characterize Interacting Protein Complex
Hunan Key Laboratory of Molecular Precision Medicine, Xiangya Hospital, Central South University, Changsha, China
Center for Medical Genetics and Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, China
Shanghai United International School Gubei Campus, Shanghai, China
- 1 Hunan Key Laboratory of Molecular Precision Medicine, Xiangya Hospital, Central South University, Changsha, China
- 2 Center for Medical Genetics and Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, China
- 3 Shanghai United International School Gubei Campus, Shanghai, China
American Journal of Molecular Biology·Volume 13 (2023)·Pages 213–226·Published 31 August 2023·DOI10.4236/ajmb.2023.134015
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Abstract
The study of the neuron has always been a fundamental aspect when it came to studying mental illnesses such as autism and depression. The protein protocadherin-9 (PCDH9) is an important transmembrane protein in the development of the neuron synapse. Hence, research on its protein interactome is key to understanding its functionality and specific properties. A newly discovered biotin ligase, TurboID, is a proximity labeler that is designed to be able to label and observe transmembrane proteins, something that previous methods struggled with. The TurboID method is verified in HEK293T cells and primary cultured mouse cortical neurons. Results have proven the validity of the TurboID method in observing PCDH9-interacting proteins.
KeywordsTurboIDPCDH9Proximity LabelingProtein InteractomeSynapse Development
- Hulpiau, P. and Van Roy, F. (2009) Molecular Evolution of the Cadherin Superfamily. The International Journal of Biochemistry & Cell Biology, 41, 349-369. https://doi.org/10.1016/j.biocel.2008.09.027
- Chen, W.V. and Maniatis, T. (2013) Clustered Protocadherins. Development, 140, 3297-3302. https://doi.org/10.1242/dev.090621
- Hamada, S. and Yagi, T. (2006) Protocadherins: Classification, Structures and Function. Seikagaku, 78, 662-671.
- Uemura, M., Furuse, T., Yamada, I., et al. (2022) Deficiency of Protocadherin 9 Leads to Reduction in Positive Emotional Behaviour. Scientific Reports, 12, Article No. 11933. https://doi.org/10.1038/s41598-022-16106-5
- Branon, T.C., Bosch, J.A., Sanchez, A.D., et al. (2018) Efficient Proximity Labeling in Living Cells and Organisms with TurboID. Nature Biotechnology, 36, 880-887. https://doi.org/10.1038/nbt.4201
- Cho, K.F., Branon, T.C., Rajeev, S., et al. (2020) Split-TurboID Enables Contact-Dependent Proximity Labeling in Cells. Proceedings of the National Academy of Sciences of the United States of America, 117, 12143-12154. https://doi.org/10.1073/pnas.1919528117
- Zhang, Y., Li, Y., Yang, X., Wen, Z., Nagalakshmi, U. and Dinesh-Kumar, S.P. (2020) TurboID-Based Proximity Labeling for in Planta Identification of Protein-Protein Interaction Networks. Journal of Visualized Experiments, No. 159. https://doi.org/10.3791/60728-v
- Schopp, I.M., Amaya Ramirez, C.C., Debeljak, J., et al. (2017) Split-BioID a Conditional Proteomics Approach to Monitor the Composition of Spatiotemporally Defined Protein Complexes. Nature Communicationsions, 8, Article No. 15690. https://doi.org/10.1038/ncomms15690
- Bruining, H., Matsui, A., Oguro-Ando, A., et al. (2015) Genetic Mapping in Mice Reveals the Involvement of Pcdh9 in Long-Term Social and Object Recognition and Sensorimotor Development. Biological Psychiatry, 78, 485-495. https://doi.org/10.1016/j.biopsych.2015.01.017
- Xiao, X., Zheng, F., Chang, H., et al. (2018) The Gene Encoding Protocadherin 9 (PCDH9), a Novel Risk Factor for Major Depressive Disorder. Neuropsychopharmacology, 43, 1128-1137. https://doi.org/10.1038/npp.2017.241