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Investigating Biological Relationships in Burkina Faso Using DNA Testing

¹ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
² Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
³ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
⁴ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
⁵ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
⁶ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
⁷ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
⁸ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
⁹ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
¹⁰ Université Joseph KI-ZERBO, Unité de Formation en Sciences de la Vie et de la Terre, Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE), Ouagadougou, Burkina Faso
¹¹ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
¹² Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
¹³ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
¹⁴ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
¹⁵ Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso
¹⁶ Centre National de la Recherche Scientifique et Technologique (CNRST), Institut de Recherche en Sciences de la Santé (IRSS), Ouagadougou, Burkina Faso
¹⁷ Faculty of Health Sciences, University Saint Thomas d’Aquin (USTA), Ouagadougou, Burkina Faso
¹⁸ Centre Hospitalier Universitaire Pédiatrie Charles De Gaulle, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso

American Journal of Molecular Biology·Volume 15 (2024)·Pages 97–109·Published 26 November 2024·DOI10.4236/ajmb.2025.151008

Abstract

DNA testing for biological relationships is becoming increasingly common in Burkina Faso. STR analysis remains the most reliable technique for resolving disputes or claims in court regarding biological relationships. This study aimed to establish the links of biological relationships between subjects by analyzing 21 STR loci. The participants were 14 individuals referred to CERBA by the Justice in 2022. Oral or blood samples were taken for each subject. DNA was extracted, and the analysis of DNA polymorphism by PowerPlex® 21 Kit (Part No. DC8902) was performed by capillary electrophoresis on the ABI PRISM 3130 sequencer. DNA profiles were compiled using the GeneMapper IDX software version 1.2. Of the fourteen subjects examined, twelve of these samples had complete genetic profiles, while the other two had partial and absent profiles. The results confirmed the inclusion of three cases of Paternity, one case of maternity, one case of a relationship of brotherhood, and the exclusion of one case of maternity and one case of a relationship of brotherhood. DNA tests improve the resolution of filiations, but they require ethical and cultural awareness and a strengthened legal framework to prevent and protect society.

KeywordsDNASTRIdentificationGenetic FingerprintBurkina Faso

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Investigating Biological Relationships in Burkina Faso Using DNA Testing

Abstract

References (32)