One of the most common cancers is Breast Cancer (BC), representing a worldwide public health concern. We previously showed estrogen receptor β (ER β ) oncosuppressor activity as significantly affected estrogen-induced early transcription and mRNA splicing in hormone-responsive MCF-7 human BC cell models. Since gene fusion phenomena are recurrent in cancer cells, we performed a transcriptomic analysis (RNAseq) aiming to assess gene fusion events in MCF-7 human BC cell models that expressed estrogen nuclear receptors α / β under early estradiol (E2) stimulation. The genomic reads sequences were aligned on GRCh38 human genome by using RNA STAR, while the star-fusion was used to detect gene fusion events. Results showed a non-significant variability regarding gene fusion events happening between estradiol-stimulated MCF-7E expressing ER β (ER β +/ER α −) and non-stimulated MCF-7noE repressing ER β expression (ER β −/ER α +), MCF-7 human BC cell models (p > 0.05). Commonly detected gene fusion events between these two (2) BC cell models result in biomarkers of several cancers and as well BC, and are characterized by intra-chromosomal interactions. Findings revealed five (5) gene fusion events specific to MCF-7noE BC cell models in which ER β gene expression is repressed (ER β −/ER α +), and recognized as breast cancer biomarkers. Interestingly, results exhibited ER β expression as inhibiting gene fusion phenomena specific to BC (BC biomarkers) in replying to estradiol (E2) stimulus in monitoring early hormone-responsive MCF-7 human BC cell models (ER β +/ER α −; MCF-7E). Overall, even if early estrogen hormone stimulation by inducing nuclear ER β has non-significant impact on gene fusion variability between MCF-7noE (ER β −/ER α +) and MCF-7E (ER β +/ER α −) BC cell line models by contrast to the alternative splicing event, our study highlighted onco-suppressor activity of ER β in hormone-responsive BC cell line model by potentially silencing several gene fusion expression recognized as BC biomarkers. However, further investigation is necessary to comprehend how ER β monitors gene fusion in BC.
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