Screening Rice ( Oryza sa tiva L.) Varieties for Resistance to Rice stripe necrosis virus (RSNV)

Abstract

Rice stripe necrosis virus (RSNV) is one of the main viruses infecting rice in Burkina Faso, causing significant damage. The objective of this work was to identify varietal resistance to Rice stripe necrosis virus (RSNV). The tests were carried out in a real environment, in the lowland of the Banfora experimental site (Tannounyan region) located at the INERA research station. Seeds of 30 improved rice varieties have been planted. For each variety, direct seeding was carried out at the rate of one (01) grain per pocket in elementary plots of 6.24 m 2 (5.2 m × 1.2 m) on five (5) rows. The spacing between the lines and between the pockets is identical and corresponds to 20 cm. NPK-based base fertilizer (14-23-14) was applied at a rate of 300 kg per ha. Three weedings were carried out and during the ripening-harvesting phase water was maintained until 14 days before harvest. Thus, through a three-replicate alpha lattice device, varietal field screening followed by impact assessment, identified 28 RSNV resistant/tolerant rice genotypes while two (02) were susceptible to the disease with significant production losses reaching 31.7% and 32.8% respectively for the cultivar WAB2066-WAT21-1-B-1-TGR2 and ARC1-20-B-1. Genotypes that have developed partial resistance and acquired total Resistance 110 days after sowing (DAS) with a production loss of between 0 and 30% can be used by producers for production and by breeders for their use in varietal improvement programs.