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Frequently cutting restriction enzymes: Clearing the fog to see the ends
Institute of Vegetal Biochemistry and Photosynthesis, CSIC-University of Seville, Seville, Spain
Institute of Vegetal Biochemistry and Photosynthesis, CSIC-University of Seville, Seville, Spain
- 1 Institute of Vegetal Biochemistry and Photosynthesis, CSIC-University of Seville, Seville, Spain
- 2 Institute of Vegetal Biochemistry and Photosynthesis, CSIC-University of Seville, Seville, Spain
American Journal of Molecular Biology·Volume 03 (2013)·Pages 59–61·Published 23 January 2013·DOI10.4236/ajmb.2013.31007
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Abstract
Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present at internal chromosomal loci in many eukaryotes. However, the biological role of these interstitial telomeric sequences (ITSs) remains unknown. The integrity of telomeric length and chromatin structure is required for telomere stability. However, the study of these telomeric features can be impeded by the presence of ITSs. Frequently cutting restriction enzymes have been revealed to be very useful tools for the study of the length and chromatin structure of telomeres independent of the presence of ITSs.
KeywordsTelomeric LengthTelomeric Chromatin StructureFrequently Cutting Restriction EnzymesArabidopsis thaliana
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