Research ArticleOpen AccessGoogle Scholar indexed
Breeding of NHase hyper-producing <i>Rhodococcus ruber</i> strain LUV<sub>30-06</sub> and verification of mutants by RAPD
College of Life Sciences and Engineering of Qiqihar University, Qiqihar, China
Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science &Technology), Ministry of Education, Tianjin, China
College of Life Sciences and Engineering of Qiqihar University, Qiqihar, China
- 1 College of Life Sciences and Engineering of Qiqihar University, Qiqihar, China
- 2 Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science &Technology), Ministry of Education, Tianjin, China
- 3 College of Life Sciences and Engineering of Qiqihar University, Qiqihar, China
American Journal of Molecular Biology·Volume 03 (2013)·Pages 108–114·Published 30 April 2013·DOI10.4236/ajmb.2013.32015
Copy link · social · email
Abstract
A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV 30-06 was bred by mutageniza tion on the starting strain CGMCC3090 with ultra violet irradiation and lithium chloride. The NHase activity of the strain LUV 30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV 30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).
Keywords<i>Rhodococcus ruber</i>NHaseMutagenesisRandom Amplified Polymorphic DNA
- Prasad, S. and Bhalla, T.C. (2010) Nitrile hydratases (NHases): At the interface of academia and industry. Biotechnology Advances, 28, 725-741. doi:10.1016/j.biotechadv.2010.05.020
- Wang, S.W. and Wang, M. (2011) Research process on microbial diversity of producing NHase and study on NHase. China Biotechnology, 31, 117-132.
- Zhang, J.L., Wang, M., Sun, H., et al. (2009) Isolation and characterization of Rhodococcus ruber CGMCC3090 that hydrolyzes aliphatic, aromatic and heterocyclic nitriles. African Journal of Biotechnology, 8, 5467-5475.
- Shimizu S, Ogawa, J. and Kataoka, M. (1997) Screening of novel microbial enzymes for the production of biologically and chemically useful compounds. Advances in Biochemical Engineering/Biotechnology, 58, 46-87. doi:10.1007/BFb0103302
- Nagasawa, T. and Yamada, H. (1990) Application of nitrile converting enzymes for the production of useful compounds. Pure & Applied Chemistry, 62, 1441-1444. doi:10.1351/pac199062071441
- Guiot, P., Ryan, M.A. and Scriven, E.F.V. (1996) Preparations and applications of nicotinic acid and nicotinamide. Chimica Oggi (Chemistry Today), 14, 55-57.
- Soma, Y., Kashima, M., Imaizumi, A., et al. (2005) Moisturizing effects of topical nicotinamide on atopic dry skin. International Journal of Dermatology, 44, 197-202. doi:10.1111/j.1365-4632.2004.02375.x
- Xin, J. and Hu, B. (1992) Nicotinamide radiosensitizing effect and its clinical application. International Journal of Radiation Medicine and Nuclear Medicine, 16, 200-203.
- Guo, J.L., Shen, H. and Li, Q. (2008) In vitro effect of nicotinamide of GLC-82 human lung adenocarcinoma cells. Guangdong Medical Journal, 29, 209-211.
- Zhang, L. (2004) Niacin and niacinamide production growth gratifying and demand foreground being expected period. Medicine Economic News, 12, 25
- Song, Y., Zhong, L.P. and Wang, M. (2009) Effect of metal ions on Rhodococcus sp. nitrile hydratase activity. Journal of Tianjing University of Science & Technology, 24, 11-14.
- Prasad, S., Raj, J. and Bhalla, T.C. (2004) Optimization of culture conditions for hyper production of nitrile hydratase of Rhodococcus rhodochrous PA-34. Indian Journal of Microbiology, 44, 251-256.
- Li, X.D., Wang, M., Zheng, Y., et al. (2010) Optimization of conditions for the biotransformation of 5-cyanovaleramide from adiponitrile by Rhodococcus ruber CGMCC-3090. 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE), Chengdu, 18-20 June 2010, 1-4.