Comparative study on some selected species of <i>Ocimum</i> genus on free radical scavenging activity and hepatoprotective activity against CCl4 induced intoxication in rats
- 1 Alcorn State University, Mississippi, USA
- 2 AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India
- 3 AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India
- 4 Alcorn State University, Mississippi, USA
Abstract
In this study, we compared the in vitro antioxidant property among the selected Ocimum species ( O . sanctum , O . americanum , O . basilicum and O . gratissimum ) and hepatoprotective activities of their extracts against CCl 4 induced intoxication. The results suggested that the four species of Ocimum genus showed variability in Phenolic content and in vitro antioxi-dant activity against DPPH, superoxide and hydroxyl radicals in the following manner: O . sanctum > O . americanum > O . basilicum > O . gratissimum respec-tively. Based on serum AST, ALT, ALP and T. Bil levels, the alcoholic extracts of Ocimum species showed a significant dose dependent (250 mg and 500 mg and 750 mg/kg p.o.) and a protective effect against CCl 4 induced hepatotoxicity in albino rats. The results further revealed that the potential hepatoprotective activity of Ocimum sanctum among the Ocimum species.
- Girish, S.A., Sudhir, G.W. and Avinash, K.D. (2004) Evaluation hepatoprotective effect of amalkadi ghrita against carbon tetrachloride-induced hepatic damage in rats. Journal of Ethnopharmacology, 90, 229-232. http://dx.doi.org/10.1016/j.jep.2003.09.037
- Sreelatha, S., Padma P.R. and Umadevi, M. (2009) Protective effects of Coriandrum sativum extacts on carbon tetrachloride-induced Hepatotoxicity in rats. Food and Chemical Toxicology 47, 702-708. http://dx.doi.org/10.1016/j.fct.2008.12.022
- Geesin, J.C., Gordon, J.S. and Bergand, R.A. (1990) Retinoids affect collagen synthesis through inhibition of ascorbate-induced lipid peroxidation in cultured human dermal fibroblasts. Archives of Biochemistry and Biophysics, 278, 350-355. http://dx.doi.org/10.1016/0003-9861(90)90270-9
- Thresiamma, K.C. and Kuttan, R. (1996) Inhibition of liver fibrosis by ellagic acid. Indian Journal of Physiology and Pharmacology, 40, 363-366.
- Singleton, V. L. and Rossi, J. A. (1965) Colorimetry of total phenolics with phosphomolybdic acid-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.
- McCord J.M. and Fridovich, I. (1969) Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprien). Journal of Biological Chemistry, 244, 6049-6055. http://dx.doi.org/10.1016/0378-5173(90)90201-E
- Elizabeth Kunchandy. and Rao, M.N.A. (1990) Oxygen radical scavenging activity of curcumin. International Journal of Pharmaceutics, 58, 237-240. http://dx.doi.org/10.1016/S0378-8741(03)00043-6
- Braca, A., Fico, G., Morelli, I., De Simone, F., Tome, F. and De Tommasi, N. (2003) Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. Journal of Ethnopharmacology, 86, 63-67.
- Reitman, S. and Frankel, S. (1957) Determination of serum glutamate oxaloacetate and glutamate pyruvic acid transaminase. American Journal of Clinical Pathology, 28, 56-63.
- King, E.J. and Armstrong, A.R. (1934) Determination of serum and bile phosphatase activity. Cannadian Medical Association Journal, 31, 56-63.
- Jendrassik, L. and Gróf, P. (1938) Vereinfachte photometrische Methoden zur Bestimmung des Blutbilirubins. Biochemische Zeitschrift, 297, 82-89.