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Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in <i>Solanum lycopersicum</i>
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
Vegetable research station, Anand Agricultural University, Anand, India
Vegetable research station, Anand Agricultural University, Anand, India
- 1 B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
- 2 B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
- 3 B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
- 4 B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
- 5 B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
- 6 B. R. D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar, India
- 7 Vegetable research station, Anand Agricultural University, Anand, India
- 8 Vegetable research station, Anand Agricultural University, Anand, India
American Journal of Molecular Biology·Volume 03 (2013)·Pages 241–247·Published 17 October 2013·DOI10.4236/ajmb.2013.34031
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Abstract
With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and to further facilitate the use of markers in tomato breeding. The present study is a step to introduce a new SSR marker (TOM-144) which was deduced after evaluation of eight microsatellite loci amongst the twenty-one different tomato cultivars. The marker selected was inherited and segregated in mendelian fashion as demonstrated in successive generation of a cross between parent cvs. H-24 x GT-2.
KeywordsFusarium WiltMolecular MarkerTomato
- Tanksley, S.D. and McCouch, S.R. (1997) Seed banks and molecular maps: Unlocking genetic potential from the wild, Science, 277, 1063-1066. http://dx.doi.org/10.1126/science.277.5329.1063
- Foolad, M.R. and Panthee, D.R. (2012) Marker-assisted selection in tomato breeding. Critical Reviews in Plant Sciences, 31, 93-123. http://dx.doi.org/10.1080/07352689.2011.616057
- Parmar, P.P., Oza, V.P., Patel, A.D., Kathiria, K.B. and Subramanian, R.B. (2011) Development of a rapid and reliable bioassay to discriminate between susceptible and resistant cultivars of tomato against Fusarium wilt. International Journal of Biosciences Agriculture and Technology, 3, 6-11.
- Oza, V., Trivedi, S., Parmar, P. and Subramanian, R.B. (2008) A simple, rapid and efficient method for isolation of genomic DNA from plant tissue. Journal of Cell and Tissue Research, 8, 1383-1386.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: A laboratory manual, 2nd Edition, Cold Spring Harbor Lab. Press, New York.
- Parmar, P., Gandhi, M. and Subramanian, R.B. (2012) Isolation and molecular characterization of avirulence gene from Indian isolates of Fusarium oxysporum f. sp. Lycopersici. Journal of Cell and Tissue Research, 12, 3061-3067. http://dx.doi.org/10.1139/g98-155
- Areshchenkova, T. and Ganal, M.W. (1999) Long tomato microsatellites are predominantly associated with centromeric regions. Genome, 42, 536-544.
- Suliman-Pollatschek, S., Kashkush, K., Shats, H. and Lavi, U. (2002) Generation and mapping of AFLP, SSRs and SNPs in Lycopersicon esculentum. Cellular Molecular Biology Letters, 7, 583-597.
- Parmar, P., Bhatt, K., Oza, V., Kathiria, K. B. and Subramanan, R.B. (2009) Microsatellite marker associated with Fusarium wilt resistance in tomato. World Journal of Agricultural Sciences, 5, 389-393.
- Lievens, B., Rep, M. and Thomma, B.P. (2008) Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest Management Science, 64, 781-788. http://dx.doi.org/10.1002/ps.1564
- Marshall, T.C., Slate, J., Kruuk, L.E.B. and Pemberton, J.M. (1998) Statistical confidence for likelihood based paternity inference in natural populations. Molecular Ecology, 7, 639-655. http://dx.doi.org/10.1046/j.1365-294x.1998.00374.x
- Dakin, E.E. and Avise, J.C. (2004) Microsatellite null alleles in parentage analysis. Heredity, 93, 504-509. http://dx.doi.org/10.1038/sj.hdy.6800545