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A method to synthesize cDNA constructs by homology based recombination cloning
Department of Genetics, University of Delhi, South Campus, New Delhi, India
Department of Genetics, University of Delhi, South Campus, New Delhi, India
- 1 Department of Genetics, University of Delhi, South Campus, New Delhi, India
- 2 Department of Genetics, University of Delhi, South Campus, New Delhi, India
American Journal of Molecular Biology·Volume 04 (2014)·Pages 16–19·Published 3 January 2014·DOI10.4236/ajmb.2014.41003
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Abstract
We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB 1 and MYB 2 genes of Arabidopsis thaliana .
KeywordsHomology Based Recombination CloningcDNA Assembly
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