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Polymerase Chain Reaction Detection of ASFV Infection in Nigerian Indigenous Pig
Institute of Agricultural Research and Training, Obafemi Awolowo University, Ile-Ife, Nigeria
Faculty of Agriculture, Animal Science Department, Obafemi Awolowo University, Ile-Ife, Nigeria
- 1 Institute of Agricultural Research and Training, Obafemi Awolowo University, Ile-Ife, Nigeria
- 2 Faculty of Agriculture, Animal Science Department, Obafemi Awolowo University, Ile-Ife, Nigeria
American Journal of Molecular Biology·Volume 04 (2014)·Pages 159–162·Published 16 July 2014·DOI10.4236/ajmb.2014.43017
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Abstract
This work investigated the presence of African Swine Fever Virus (ASFV) in Nigerian Indigenous Pig (NIP) using Polymerase Chain Reaction (PCR) screening method on the extracted DNA from the pig blood. NIP population selected from south western Nigeria were used for this study. ASFV infected blood samples collected from the University of Ibadan were used as positive control. Adiscrete and specific band was observed in both NIP and the infected samples show the presence of ASFV in NIP. The bands were of the expected size 278 base pairs (bp). The implication of this finding is that NIPs have domiciled ASFV without showing any clinical symptoms.
KeywordsNigerian Indigenous PigPolymerase Chain ReactionAfrican Swine Fever
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