Isolation and Characterization of Hydrocarbon Utilizing Yeast (HUY) Isolates from Palm Wine
- 1 Department of Microbiology, University of Port Harcourt, Port Harcourt, Nigeria
- 2 Department of Environmental Science, Federal University of Petroleum Resources, Effurun, Nigeria
- 3 Department of Microbiology, University of Port Harcourt, Port Harcourt, Nigeria
Abstract
Several studies have reported on the capabilities of different yeasts to utilize hydrocarbons hence the need to ascertain the petroleum hydrocarbon biodegradation potential of yeast isolated from palm wine. The study aimed at isolation, characterization and testing of yeasts from palm wine for biodegradation of petroleum hydrocarbons. Hydrocarbon utilizing yeasts (HUY) were isolated from palm wine, a rich sugar substrate from a non-oil impacted environment using Bacto Bushnell Haas broth. The medium composed of 0.2 g MgSO 4 , 0.02 g CaCl 2 , 1 g KH 2 PO 4 , 1 g K 2 HPO 4 , 0.05 g FeCl 2 , 1 g/L NH 4 NO 3 , pH (7.2 ± 0.2) and mineral salt broth comprising of 4.4 g KH 2 PO 4 , 8 g (NH 4 ) 2 SO 4 , 2 g Na 2 HPO 4 · H 2 O, MgSO 4 · 7H 2 O, 0.1 g FeSO 4 , 0.01 g/L CaCl 2 , pH (4.5 ± 0.2) supplemented with palm wine (0.5%) and crude oil (1%). Yeasts isolated from an enriched medium and palm wine showed similarities. Yeast isolates were further screened for hydrocarbon degrading potentials using the Bacto Bushnell Haas broth containing 2, 6-dichlorophenol indophenols as the indicator agent. The isolates were identified using microscopic, macroscopic, fermentation and molecular characteristics as Candida adriatica (ZIM 2468) and Candida taoyuanica (MYA-4700).
- Das, N. and Chandran, P. (2011) Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. Biotechnology Research International, 2011, 941810. http://dx.doi.org/10.4061/2011/941810
- Kurzman, C.P. and Piskur, J. (2006) Taxonomy and Phylogenetic Diversity among the Yeasts. In: Sunnerhagen, P. and Piskur, J., Eds., Comparative Genomics: Using Fungi as Models, Berlin, Springer, 29-46. http://dx.doi.org/10.1007/b106654
- Walker, K., Skelton, H. and Smith, K. (2002) Cutaneous Lesions Showing Giant Yeast Forms of Blastomycesdermatitidis. Journal of Cutaneous Pathology, 29, 616-618. http://dx.doi.org/10.1034/j.1600-0560.2002.291009.x
- Frazier, C.W. and Westhoff, C.W. (2008) Food Microbiology. 4th Edition, Tata McGraw Hill Education Private Limited, New Delhi, 22-39.
- Kurzman, C.P. and Fell, J.W. (2005) Biodiversity and Ecophysiology of Yeasts. In: Gabor, P. and de la Rosa, C.L., Eds., The Yeast Handbook, Berlin, Springer, 11-30.
- He, F. and Chen, Y.T. (2005) Cloning and Heterologous Expression of NADPH Cytochrome P450 Oxidoreductase Genes from an Industrial Dicaboxylic Acid-Producing Candida tropicalis. Yeast, 22, 481-491. http://dx.doi.org/10.1002/yea.1227
- Csutak, O., Stoica, I., Ghindea, R., Tanase, A. and Vassu, T. (2010) Insights on Yeasts Bioremediation Process. Romanian Biotechnological Letters, 15, 5066-5071.
- Suh, S.O., McHugh, J.V., Pollock, D.D. and Blackwell, M. (2005) The Beetle Gut: A Hyper Diverse Source of Novel Yeasts. Mycological Research, 109, 261-265. http://dx.doi.org/10.1017/S0953756205002388
- Nwanchukwu, I.N., Ibekwe, V.I., Nwabueze, R.N. and Anyanwu, B.N. (2006) Characterization of Palm Wine Yeast Isolates for Industrial Utilisation. African Journal of Biotechnology, 5, 1725-1728.
- Enwefa, R., Uwajeh, M. and Oduh, M. (1992) Some Studies on Nigerian Palm Wine with Special Reference to Yeasts. Acta Biotechnologica, 12, 117-125. http://dx.doi.org/10.1002/abio.370120208
- Okerentugba, P.O. and Ezeronye, O.U. (2003) Petroleum Degrading Potentials of Single and Mixed Microbial Cultures Isolated from Rivers and Refinery Effluent in Nigeria. African Journal of Biotechnology, 2, 288-292. http://dx.doi.org/10.5897/AJB2003.000-1058
- Tuntiwongwanich, S. and Leenanon, B. (2009) Morphology and Identification of Yeasts Isolated from Toddy Palm in Thailand. Journal of Microscopy Society of Thailand, 23, 34-37.