Isolation and Functional Analysis of the Regulation of Branching by Isopentenyl Transferase Gene CmIPT1 in <i>Chrysanthemum morifolium </i>cv. ‘Jinba’
- 1 Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing, China
- 2 Department of Ornamental Horticulture, Anhui Agricultural University, Hefei, China
- 3 Department of Ornamental Horticulture, Anhui Agricultural University, Hefei, China
- 4 Department of Ornamental Horticulture, Anhui Agricultural University, Hefei, China
- 5 Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing, China
Abstract
Today’s chrysanthemums are highly evolved flowering plants and they are considered as one of the most important ornamental cut flowers. In this research an isopentenyl transferase gene named CmIPT 1 was isolated from Chrysanthemum morifolium cv. ‘ Jinba ’ using RACE and RT-PCR methods. The full cDNA sequence of CmIPT 1 was 873 bp which encoded a deduced protein of 290 amino acids. It contained GxxGxGKS which is a conserved sequence of the typical domain of IPT family. The phylogenetic tree analysis of CmIPT 1 in Chrysanthemum morifolium cv. ‘ Jinba ’ shows that it has the closest relationship with CcIPT1 in Cynara cardunculus var. scolymus . Expression of CmIPT 1 was higher in stems and apex, whereas it was lower in leaves and roots. And the overexpression of CmIPT 1 obviously increased the number of rosette branches in Arabidopsis. Here, in our study, we showed that CmIPT 1 is a positive regulator of branch development in Chrysanthemum and may play a key role in regulating lateral branch formation of Chrysanthemum plants.
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