<i>In Vitro</i> Chromosomal Aberration Frequency by Electrofishing on <i>Poecilia latipinna</i> (Sailfin Molly) Fishes in Southern of Iraq

Abstract

The present stud ies describe Chromosomal aberration effects of electrofishing, which were evaluated on Poecilia latipinna , located in Shat Al-Arab river in Al-ga r mma city (south of Iraq). The electrofishing derive used in work is simulated to that used in the commercial fishing. The apparatus generates voltage ranged from 40 to 280 volts. Nine bearers of Poecilia latipinna sailfin molly fish in chromosomal analysis were divided into three treatments. The first were a control, the fishes of the second were exposed to 110 volts (10 seconds) , and final groups were exposed to 110 volts (15 seconds). Mitotic index of t he electrofishing with a control for each group decreased with increasing exposed time in somatic cell kidney tissue of Poecilia latipinna . The chromosome aberration analysis revealed a significant increase in the most frequent aberration per 150 metaphase in analyzed groups (1.33 in T1 groups, 39 . 33 in T2 groups) was chromosome break, fragment, range chromosome, Sticky chromosome mean, were higher in comparison to non exposed electrical shock fishing groups (control groups T1). At the same time, it showed a higher positive correlation of total chromosome aberration frequencies between T1 and T2 groups, while, all fishes died in T3 groups. According to our results, we represented the first record in Iraq.