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Modification of Periodate Oxidation Method to Produce HRP-IgG Conjugate and Test its Stability Overtime
Biotechnology Department, Benta Pharma Industries, Dbayeh, Lebanon
Faculty of Pharmacy, Lebanese University, Rafic Hariri University Campus, Hadath, Lebanon
Biotechnology Department, Benta Pharma Industries, Dbayeh, Lebanon
- 1 Biotechnology Department, Benta Pharma Industries, Dbayeh, Lebanon
- 2 Faculty of Pharmacy, Lebanese University, Rafic Hariri University Campus, Hadath, Lebanon
- 3 Biotechnology Department, Benta Pharma Industries, Dbayeh, Lebanon
American Journal of Molecular Biology·Volume 09 (2019)·Pages 52–63·Published 5 March 2019·DOI10.4236/ajmb.2019.92005
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Abstract
Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary conjugate was produced using a modified periodate oxidation method. The obtained conjugate was tested in the quality control techniques of therapeutic proteins. To determine the working dilution, titration of the prepared conjugate was performed in Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and found to be 1:5000. This dilution was further tested in Western Blot analysis. The secondary conjugate was kept at 4 ° C for one month and its stability was verified by Western Blot and Indirect ELISA techniques.
KeywordsAntibodyConjugationHorseradish Peroxidase (HRP)Indirect ELISAPeriodate-Mediated Glycoprotein Oxidation
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