Fatty Acid Profiling of Polyethylene Glycol Adapted and Un-Adapted Cell Lines of <i>Oryza sativa</i> L.cv. Swat-1 under Temperature Stress — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Fatty Acid Profiling of Polyethylene Glycol Adapted and Un-Adapted Cell Lines of <i>Oryza sativa</i> L.cv. Swat-1 under Temperature Stress
Institute of Crop Science, National Engineering Laboratory for Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, Beijing, China
,
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
,
Institute of Biotechnology and Genetic Engineering, University of Agriculture, Peshawar, Pakistan
1 Institute of Crop Science, National Engineering Laboratory for Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, Beijing, China
2 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
3 Institute of Biotechnology and Genetic Engineering, University of Agriculture, Peshawar, Pakistan
Higher plants can adapt to abiotic stress to a certain degree. In this study, the impact of temperature stress on osmotic stress adapted and un-adapted cell lines of rice ( Oryza sativa L.cv Swat-1) was observed. For the change in proline content, relative growth rate, saturated and unsaturated fatty acid were evaluated. The cell lines were incrementally adapted to 20% polyethylene glycol. The adapted lines showed significantly higher growth rate and proline content as compared to the un-adapted cell lines on temperature stress. Among saturated fatty acids palmitic acid (C16:0), stearic acid (C18:0) and myristic acid (C14:0) were the prominent fatty acids detected while among unsaturated fatty acid Oleic acid (C18:1c) and Linoleic acid (C18:2c) were the major fatty acids found. Under low temperature stress the percentage of saturated fatty acids was found to be lower (53%) in adapted cell line as compared to the un-adapted cell line (63%) while the percentage of saturation increased (83%) in adapted line under high temperature stress as compared to un-adapted line (70%). On the other hand at low temperature stress the percent level of unsaturated fatty acids in the adapted line was higher (48%) than the un-adapted cell line (37%). In conclusion, adaptation to one abiotic stress confers co-tolerance to the other abiotic stresses. Fatty acids saturation level could be a crucial factor in the plant ability to tolerate heat and cold stress.
Flowers, T.J. and Yeo, A.R. (1995) Breeding for Salinity Resistance in Crop Plants: Where Next? Functional Plant Biology, 22, 875-884. https://doi.org/10.1071/PP9950875
Ashraf, M. and Harris, P.J.C. (2004) Potential Biochemical Indicators of Salinity Tolerance in Plants. Plant Science, 166, 3-16. https://doi.org/10.1016/j.plantsci.2003.10.024
Wang, W.-X., Vinocur, B. and Altman, A.J.P. (2003) Plant Responses to Drought, Salinity and Extreme Temperatures: towards Genetic Engineering for Stress Tolerance. Planta, 218, 1-14. https://doi.org/10.1007/s00425-003-1105-5
Yardanov, I., Velikova, V. and Tsonev, T. (2003) Plant Responses to Drought and Stress Tolerance. Bulgarian Journal of Plant Physiology, Special Issue 2003, 187-206.
Seki, M., Umezawa, T., Urano, K. and Shinozaki, K. (2007) Regulatory Metabolic Networks in Drought Stress Responses. Current Opinion in Plant Biology, 10, 296-302. https://doi.org/10.1016/j.pbi.2007.04.014
Nayyar, H. and Walia, D.P. (2003) Water Stress Induced Proline Accumulation in Contrasting Wheat Genotypes as Affected by Calcium and Abscisic Acid. Biologia Plantarum, 46, 275-279. https://doi.org/10.1023/A:1022867030790
Mahajan, S. and Tuteja, N. (2005) Cold, Salinity and Drought Stresses: An Overview. Archives of Biochemistry and Biophysics, 444, 139-158. https://doi.org/10.1016/j.abb.2005.10.018
Elkahoui, S., Smaoui, A., Zarrouk, M., Ghrir, R. and Limam, F.J.P. (2004) Salt-Induced Lipid Changes in Catharanthus roseus Cultured Cell Suspensions. Phytochemistry, 65, 1911-1917. https://doi.org/10.1016/j.phytochem.2004.06.021
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Shah, A.H., Shah, S.H., Ahmad, H., Swati, Z.A., Abbasi, F.M., Farhatullah, and Shah, A.H. (2012) Adaptation to Polyethylene Stress Maintains Totipotency of Cell Lines of Oryza sativa L. cv. Swat-1 for a Longer Period. Pakistan Journal of Botany, 44, 313-316.
Shah, A.H., Shah, S.H., Ahmad, H., Swati, Z.A., Afzal, M., Aiman, U. and Khalid, Q. (2012) The Phenomenon of Cross Tolerance in Osmotically and Ionically Adapted Rice (Oryza sativa L.) Cell lines. African Journal of Biotechnology, 11, 713-717.
Singh, T., Aspinall, D., Paleg, L. and Boggess, S. (1973) Stress Metabolism II. Changes in Proline Concentration in Excised Plant Tissues. Australian Journal of Biological Sciences, 26, 57-64. https://doi.org/10.1071/BI9730057
Bates, L.S., Waldren, R.P. and Teare, I.J.P. (1973) Rapid Determination of Free Proline for Water-Stress Studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060
Bligh, E.G. and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917. https://doi.org/10.1139/o59-099
Shah, S., Wainwright, S. and Merrett, M.J. (1990) The Interaction of Sodium and Calcium Chlorides and Light on Growth, Potassium Nutrition, and Proline Accumulation in Callus Cultures of Medicago sativa L. New Phytologist, 116, 37-45. https://doi.org/10.1111/j.1469-8137.1990.tb00508.x
Wahid, A. and Close, T.J. (2007) Expression of Dehydrins under Heat Stress and Their Relationship with Water Relations of Sugarcane Leaves. Biologia Plantarum, 51, 104-109. https://doi.org/10.1007/s10535-007-0021-0
Murakami, Y., Tsuyama, M., Kobayashi, Y., Kodama, H. and Iba, K. (2000) Trienoic Fatty Acids and Plant Tolerance of High Temperature. Science, 287, 476-479. https://doi.org/10.1126/science.287.5452.476
da Cruz, R.P., Golombieski, J.I., Bazana, M.T., Cabreira, C., Silveira, T.F. and da Silva, L.P. (2010) Alterations in Fatty Acid Composition Due to Cold Exposure at the Vegetative Stage in Rice. Brazilian Society of Plant Physiology, 22, 199-207. https://doi.org/10.1590/S1677-04202010000300007
Hur, J.-H., Jung, K.-H., Lee, C.-H. and An, G. (2004) Stress-Inducible OsP5CS2 Gene Is Essential for Salt and Cold Tolerance in Rice. Plant Science, 167, 417-426. https://doi.org/10.1016/j.plantsci.2004.04.009
Zhang, M., Barg, R., Yin, M., Gueta-Dahan, Y., Leikin-Frenkel, A., Salts, Y., Shabtai, S. and Ben-Hayyim, G. (2005) Modulated Fatty Acid Desaturation via Overexpression of Two Distinct ω-3 Desaturases Differentially Alters Tolerance to Various Abiotic Stresses in Transgenic Tobacco Cells and Plants. The Plant Journal, 44, 361-371. https://doi.org/10.1111/j.1365-313X.2005.02536.x
Pearcy, R.W. (1978) Effect of Growth Temperature on the Fatty Acid Composition of the Leaf Lipids in Atriplex lentiformis (Torr.) Wats. Plant Physiology, 61, 484-486. https://doi.org/10.1104/pp.61.4.484
Raison, J.K., Roberts, J.K. and Berry, J.A. (1982) Correlations between the Thermal Stability of Chloroplast (Thylakoid) Membranes and the Composition and Fluidity of Their Polar Lipids upon Acclimation of the Higher Plant, Nerium oleander, to Growth Temperature. Biochimica et Biophysica Acta (BBA)-Biomembranes, 688, 218-228. https://doi.org/10.1016/0005-2736(82)90597-1
Cossins, A.R. (1994) Temperature Adaptation of Biological Membranes.
Wu, J.-L., Seliskar, D.M. and Gallagher, J.L. (1998) Stress Tolerance in the Marsh Plant Spartina patens: Impact of NaCl on Growth and Root Plasma Membrane Lipid Composition. Physiologia Plantarum, 102, 307-317. https://doi.org/10.1034/j.1399-3054.1998.1020219.x