Tannin-Rich Extract of <i>Chasmanthera dependens</i> Stem Potential in Piroxicam-Induced Nephrotoxicity in Adult Male Wistar Rats — Oak Academic Publishing
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Tannin-Rich Extract of <i>Chasmanthera dependens</i> Stem Potential in Piroxicam-Induced Nephrotoxicity in Adult Male Wistar Rats
Department of Biochemistry, Bowen University, Iwo, Nigeria
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Department of Biochemistry, Bowen University, Iwo, Nigeria
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Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ife, Nigeria
1 Department of Biochemistry, Bowen University, Iwo, Nigeria
2 Department of Biochemistry, Bowen University, Iwo, Nigeria
3 Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ife, Nigeria
Piroxicam is commonly used as anti-inflammatory and pain relieving drug; however, its side effects include fluid retention, renal damage and heart failure. This study aimed at evaluating the nephroprotective role of different doses of the tannin-rich extract of Chasmanthera dependens stem (TRECDS) on piroxicam-induced nephrotoxicity in adult male Wistar rats. Thirty-two adult rats were divided into four groups of eight rats per group and treated orally for ten days. Rats in group one received 0.5 ml normal saline (0.9% v/v) and served as normal control group. Rats in group two received 20 mg/kg body weight piroxicam alone. Rats in groups three and four received 20 mg/kg body weight of piroxicam with concomitant administration of 200 and 400 mg/kg body weight of TRECDS. At the expiration of the experiment, rats were sacrificed and the kidney was removed. Renal function was evaluated. The results showed that administration of piroxicam alone caused a significant elevation in the serum concentrations of albumin, creatinine, total protein, urea concentrations and the activity of renal nucleotidase with a reduction in the activity of glucose-6-phosphate dehydrogenase (G6PD) when compared to normal control (p < 0.05). Furthermore, renal tissue from the piroxicam alone treated group revealed a significant decrease in the activities of renal superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase as well as reduced glutathione with concomitant increase in lipid peroxidation and hydrogen peroxide generation. In addition, histological assessment of the renal tissue showed noticeable damage in piroxicam alone treated group. However, concomitant administration of TRECDS showed a dose-dependent reduction in the concentrations and the activity of the kidney markers with significant increase in the activities of G6PD and restores the antioxidant status of the kidney. The results show the nephroprotective potential of TREDS against piroxicam-induced renal damage.
Gambaro, G. and Perazella, M.A. (2003) Adverse Renal Effects of Anti-Inflammatory Agents: Evaluation of Selective and Nonselective Cyclooxygenase Inhibitors. Journal of International Medicine, 253, 643-652. https://doi.org/10.1046/j.1365-2796.2003.01146.x
Neha, R., Munna, S. and Sanghamitra, R. (2015) Piroxicam, a Traditional Non-Steroidal Anti-Inflammatory Drug (NSAID) Causes Apoptosis by ROS Mediated Akt Activation. Pharmacological Reports, 67, 1215-1223. https://doi.org/10.1016/j.pharep.2015.05.012
Liu, C.M., Ma, J.Q. and Sun, Y.Z. (2010) Quercetin Protects the Rat Kidney against Oxidative Stress-Mediated DNA Damage and Apoptosis Induced by Lead. Environmental Toxicology and Pharmacology, 30, 264-271. https://doi.org/10.1016/j.etap.2010.07.002
Adedara, I.A., Teberen, R., Ebokaiwe, A.P., Ehwerhemuepha, T. and Farombi, E.O. (2012) Induction of Oxidative Stress in Liver and Kidney of Rats Exposed to Nigerian Bonny Light Crude Oil. Environmental Toxicology, 27, 372-379. https://doi.org/10.1002/tox.20660
Weir, M.R. (2002) Renal Effects of Nonselective NSAIDs and Coxibs. Cleveland Clinic Journal of Medicine, 69, SI53-SI58. https://doi.org/10.3949/ccjm.69.Suppl_1.SI53
Harris, R.C. (2006) COX-2 and the Kidney. Journal of Cardiovascular Pharmacology, 47, S37-S42. https://doi.org/10.1097/00005344-200605001-00007
Buer, J. (2104) Origins and Impact on the Term ‘NSAID’. Inflammopharmacology, 22, 263-267. https://doi.org/10.1007/s10787-014-0211-2
Omudhome, O. (2017) Piroxicam. http://www.medicinenet.com
Nose, K. (2000) Role of Reactive Oxygen Species in the Regulation of Physiological Functions. Biological and Pharmaceutical Bulletin, 23, 897-903. https://doi.org/10.1248/bpb.23.897
Polidori, M.C., Stahl, W., Eichler, O., Niestroj, I. and Sies, H. (2001) Profiles of Antioxidants in Human Plasma. Free Radical Biology and Medicine, 30, 456462. https://doi.org/10.1016/B978-0-444-50957-4.50037-5
Dey, A. and Loukshmanan, J. (2013) The Role of Antioxidant and Other Agents in Alleviating Hyperglycemia-Mediated Oxidative Stress and Injury in Liver. Food Function, 4, 1148-1184. https://doi.org/10.1039/c3fo30317a
Channabasava, G.M., Chandrappa, C.P. and Umashankar, T. (2015) Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus micranthus and Their in-Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats. Journal of Diabetes Metabolism, 6, 5-14.
Adejuwon, S.A., Omirinde, J.O., Ebokaiwe, A.P., Aina, O.O., Adenipekun, A. and Farombi, E.O. (2014) Radiation-Induced Testicular Injury and Its Amelioration by Telfairia occidentalis. British Journal of Medicine Medical Research, 4, 7-18. https://doi.org/10.9734/BJMMR/2014/8235
Rasikh, J., Mohd, A., Qudsia, N. and Rasheela, J. (2012) Role of Antioxidant Herbal Drugs in Renal Disorders: An Overview. Free Radicals and Antioxidants, 2, 2-5. https://doi.org/10.5530/ax.2012.2.2
Mosango, D. (2008) Chasmanthera dependens Hochst. Record from Protabase. In: Schmelzer, G.H. and Gurib-Fakim, A., Eds., PROTA (Plant Resources of Tropical Africa/Ressourcesvégétales de l’Afriquetropicale), Wageningen, Netherlands, 23-45.
Ogunlesi, M., Okei, W. and Ademoye, M. (2008) Medicinal Plants Used in Treating Eye Infections in Nigeria. In: Odugbemi, T., Ed., A Textbook of Medicinal Plants from Nigeria, University of Lagos Press, Lagos, 299-317.
Morebise, O., Awe, E.O., Makinde, J.M. and. Olajide, O.A. (2001) Evaluation of the Anti-Inflammatory and Analgesic Properties of Chasmanthera dependens Leaf Methanol Extract. Fitoterapia, 72, 497-502. https://doi.org/10.1016/S0367-326X(01)00274-X
Fatokun, O.T., Wojuola, T.E., Esievo, K.B. and Kunle, O.F. (2016) Medicinal Plants Used in the Management of Asthma: A Review. European Journal of Pharmaceutical and Medical Research, 3, 82-92
Public Health Service (1996) Policy on Humane Care and Use of Laboratory Animals. US Department of Health and Human Services, Washington DC.
Fawcett, J.K. and Scott, J.E. (1960) A Rapid and Precise Method for the Determination of Urea. Journal of Clinical Pathology, 13, 156-159. https://doi.org/10.1136/jcp.13.2.156
Henry, R.J. (1974) Clinical Chemistry, Principles and Techniques. 2nd Edition, Harper and Row, Hagerstown, MD.
Goodland, G.A.J. and Clark, C.M. (1982) Alteration in Hepatic 5,-Nucleotidase in Tumor Bearing Rat. Enzyme, 27, 119-123. https://doi.org/10.1159/000459035
Misra, H.P. and Fridovich, I. (1972) The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase. The Journal of Biological Chemistry, 247, 3170-3175.
Clairborne, A. (1995) Catalase activity. In: Greewald, A.R., Ed., Handbook of Methods for Oxygen Radical Research, CRC Press, Boca Raton, FL, 237-242.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein Measurement with the Folin Phenol Reagent. The Journal of Biological Chemistry, 193, 265-275.
Jollow, D.J., Mitchell, J.R., Zampaglione, N. and Gillette, J.R. (1974) Bromobenzene Induced Liver Necrosis. Protective Role of Glutathione and Evidence for 3,4 Bromobenzene Oxide as the Hepatotoxic Metabolite. Pharmacology, 11, 151-169. https://doi.org/10.1159/000136485
Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G. and Hoekstra, W.G. (1973) Selenium: Biochemical Role as a Component of Glutathione Peroxidase. Science, 179, 588-590. https://doi.org/10.1126/science.179.4073.588
Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974) Glutathione-S-Transferases. The First Enzymatic Step in Mercapturic Acid Formation. Journal of Biological Chemistry, 249, 7130-7139.
Wolff, S.P. (1994) Ferrous Ion Oxidation in Presence of Ferric Ion Indicator Xylenol Orange for Measurement of Hydroperoxides. Methods in Enzymology, 233, 182-189. https://doi.org/10.1016/S0076-6879(94)33021-2
Farombi, E.O., Tahnteng, J.G., Agboola, A.O., Nwankwo, J.O. and Emerole, G.O. (2000) Chemoprevention of 2-Acetylaminofluorene-Induced Hepatotoxicity and Lipid Peroxidation in Rats by Kolaviron; A Garcinia Kola Seed Extract. Food and Chemical Toxicology, 38, 535-541. https://doi.org/10.1016/S0278-6915(00)00039-9
Songur, A., Akpolat, N., Kus, I., Ozen, O.A., Zararsiz, I. and Sarsilmaz, M. (2003) The Effects of the Inhaled Formaldehyde during the Early Postnatal Period in the Hippocampus of Rats: Amorphological and Immunohistochemical Study. Neuroscience Research Communications, 33, 168-178. https://doi.org/10.1002/nrc.10093
Tanti-Azizah, S., Rima, M., Peppy, W. and Yuyun-Puji, L. (2014) The Protective Effect of Sarung semut Tubers Infusion on Gentamicin-Piroxicam Induced Nephrotoxicity in Rats. Indonesian Journal of Pharmacy, 25, 91-97. https://doi.org/10.14499/indonesianjpharm25iss2pp91
Mongi, S., Mahfoud, M., Amel, B., Kamel, J. and Abdelfattah, F. (2011) Protective Effects of Vitamin C against Haematological and Biochemical Toxicity Induced by Deltamethrin in Male Wistar Rats. Ecotoxicological and Environmental Safety, 74, 1765-1769. https://doi.org/10.1016/j.ecoenv.2011.04.003
Christian, J., Wolfgang, W. and Michael, J. (2010) Hypoalbuminemia and Acute Kidney Injury: A Meta-Analysis of Observational Clinical Studies. Intensive Care Medicine, 36, 1657-1665. https://doi.org/10.1007/s00134-010-1928-z
Kore, K. (2011) RP-HPLC Simultameous Nephroprotective Role of Aegle marelos Extract. International Journal of Research in Pharmacy and Chemistry, 1, 51-56.
Spencer, N. and Stanton, R. (2017) Glucose-6-Phosphate Dehydrogenase and the Kidney. Current Opinion Nephrology and Hypertension, 26, 43-49. https://doi.org/10.1097/MNH.0000000000000294
Ozyurt, B., Gulec, M., Ozyurt, H., Ekici, F., Atis, O. and Akbas, A. (2006) The Effect of Antioxidant Caffeic Acid Phenethyl Ester (CAPE) on Some Enzyme Activities in Cisplatin-Induced Nephrotoxicity in Rats. European Journal of General Medicine, 3, 167-172. https://doi.org/10.29333/ejgm/82401
Isabel, C., Klaus, H. and Hayo, C. (2012) Role of Ecto-5’-Nucleotidase (CD73) in the Development of Renal Fibrosis. The FASEB Journal, 26, 2-3.
Khan, H. (2009) Studies on the Protective Effect of Green Tea against Cisplatin-Induced Nephrotoxicity. Pharmaceutical Research, 60, 382-391. https://doi.org/10.1016/j.phrs.2009.07.007
KDIGO, Clinical Practice Guideline for the Diagnosis, Evaluation, Prevention, and Treatment of Chronic Kidney Disease-Mineral and Bone Disorder (CKD-MBD) (2009) Introduction and Definition of CKD-MBD and the Development of the Guideline Statements. Kidney International Supplement, 76, S3-S8.
Lau, K. (1989) Phosphate Excess and Progressive Renal Failure: The Precipitation-Calcification Hypothesis. Kidney International, 36, 918-937. https://doi.org/10.1038/ki.1989.281
Bover, J., Evenepoel, P., Ureña-Torres, P., Vervloet. M.G., Brandenburg, V., Mazzaferro, S., Covic, A., Goldsmith, D., Massy, Z.A. and Cozzolino, M. (2015) Pro: Cardiovascular Calcifications Are Clinically Relevant. Nephrology and Dialysis Transplant, 30, 345-551. https://doi.org/10.1093/ndt/gfv020
Rodriguez, C., Mayo, J.C., Sainz, R.M., et al. (2004) Regulation of Antioxidant Enzymes: A Significant Role for Melatonin. Journal of Pineal Research, 36, 1-9. https://doi.org/10.1046/j.1600-079X.2003.00092.x
Adedara I.A. and Farombi, E.O. (2010) Induction of Oxidative Damage in the Testes and Spermatozoa and Hematotoxicity in Rats Exposed to Multiple Doses of Ethylene Glycol Monoethyl Ether. Human and Experimental Toxicology, 29, 801-812. https://doi.org/10.1177/0960327109360115
Masella, R., Di Benedetto, R., Vari, R., Filesi, C. and Giovannini, C. (2005) Novel Mechanisms of Natural Antioxidant Compounds in Biological Systems: Involvement of Glutathione and Glutathione Related Enzymes. Journal of Nutritional Biochemistry, 16, 577-586. https://doi.org/10.1016/j.jnutbio.2005.05.013
Kohen, R. and Nyska, A. (2002) Oxidation of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, and Methods for Their Quantification. Toxicologic Pathology, 30, 620-650. https://doi.org/10.1080/01926230290166724
Marnett, L.J. (1999) Lipid Peroxidation—DNA Damage by Malondialdehyde. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 424, 83-95. https://doi.org/10.1016/S0027-5107(99)00010-X