<i>Tithonia diversifolia</i> Mulch Stimulates the Growth of Plantain PIF Seedlings and Induces a Less Susceptibility to <i>Mycosphaerella fijiensis</i> in the Nursery — Oak Academic Publishing
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<i>Tithonia diversifolia</i> Mulch Stimulates the Growth of Plantain PIF Seedlings and Induces a Less Susceptibility to <i>Mycosphaerella fijiensis</i> in the Nursery
Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon
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Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon
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Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon
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Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
1 Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon
2 Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon
3 Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon
4 Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Plantain is an important crop that serves as a staple food and contributes significantly to income generation for millions of people in tropical and sub-Saharan Africa. Its cultivation faces the main constraint of seedlings unavailability in quantity and quality, essential for the creation of new plantations. The advent and popularization of the plantlets from stem bits (PIF) in the 2000s raised hopes for solving this problem. However, after about ten years, the PIF has shown some problems limiting its adoption and should be improved for more efficiency. The amendment of PIF substrate production with Tithonia diversifolia could be an alternative to seedlings ’ unavailability. This study aims to evaluate the potential stimulative effect of T . diversifolia mulch on plantain PIF seedlings growth and protection against black Sigatoka disease ( BSD )caused by Mycosphaerella fijiensis . The parameters of vegetative growth stages and biomarkers accumulation were assessed in sterilized substrate and non-sterilized substrate conditions. T. diversifolia mulch treatment increases the germination rate, the number of shoots, the height and the diameter of shoots, the leaf area as well as the seedlings roots, but it also protects the seedlings against BSD up to about 81% compared to the control seedlings. It also enhances the accumulation of biomarkers such as proteins, polyphenols content and defense-related enzymes (peroxidase, polyphenol oxidase and glucanase). T. diversifolia mulch seems to act in PIF seedlings production as a vital stimulator. It can therefore be taken as a tool for a more sustainable and resilient agriculture, and for poverty alleviation of poor small holder farmers.
Ewané, C.A., Ndongo, F., Ngoula, K., TeneTayo, P.M., Opiyo, S.O. and Boudjeko, T. (2019) Potential Biostimulant Effect of Clam Shells on Growth Promotion of Plantain PIF Seedlings (var. Big Ebanga & Batard) and Relation to Black Sigatoka Disease Susceptibility. American Journal of Plant Science, 10, 1763-1788. https://doi.org/10.4236/ajps.2019.1010125
FAO (2018) Food and Agriculture Organization of the United Nations. FAO Statistics: Bananas. http://www.fao.org/faostat/en/#data/QC
Kwa, M. (2003) Activation de bourgeons latents et utilisation de fragments de tige du bananier pour la propagation en masse de plants en conditions horticoles in vivo. Fruits, 58, 315-328. https://doi.org/10.1051/fruits:2003018
Onautshu, O.D. (2013) Caractérisation des populations de Mycosphaerella fijiensis et épidémiologie de la cercosporiose noire du bananier (Musa spp.) dans la région de Kisangani-République Démocratique du Congo. Thèse de doctoratès science, Université Catholique de Louvain.
Ewané, C.A., Chillet, M., Castelan, F., Brostaux, Y., Lassois, L., Ngando, E.J., Hubert, O., Chilin-Charles, Y., Lepoivre, P. and de Lapeyre de Bellaire, L. (2013) Impact of the Extension of Black Leaf Streak Disease on Banana Susceptibility to Post-Harvest Diseases. Fruits, 68, 351-365. https://doi.org/10.1051/fruits/2013081
Ewane, C.A., Milawe, C.A., Ndongo, E.F. and Boudjeko T. (2020) Influence of Clam Shells and Tithonia diversifolia Powder on Growth of Plantain PIF Seedlings (var. French) and Their Sensitivity to Mycosphaerella fijiensis. African Journal of Agricultural Research, 15, 393-411. https://doi.org/10.1051/fruits/2013081
Chagas-Paula, D.A., Oliveira, R.B., Rocha, B.A. and da Costa, F.B. (2012) Ethnobotany, Chemistry and Biological Activities of the Genus Tithonia (Asteraceae). Chemistry and Biodiversity, 9, 210-235. https://doi.org/10.1002/cbdv.201100019
Umar, O.B., Alex, R.D. and Obukohwo, E.E. (2015) Phytochemical and Proximate Composition of Tithonia diversifolia (Hemsl.) A. Gray. Annals Food Science and Technology, 16, 195-200.
Kerebba, N., Oyedeji, A.O., Byamukama, R., Kuria, S.K. and Oyedeji, O.O. (2019) Pesticidal Activity of Tithonia diversifolia (Hemsl.) A. Gray and Tephrosiavogelii (Hook f.); Phytochemical Isolation and Characterization: A Review. South African Journal of Botany, 121, 366-376. https://doi.org/10.1016/j.sajb.2018.11.024
Pirovani, P.C., Heliana, A.S.C., Regina, C.R., Dayane, S.G., Fatima, C.A. and Fabienne, M. (2008) Protein Extraction for Proteome Analysis from Cacao Leaves and Meristems, Organs Infected by Moniliophthora perniciosa, the Causal Agent for the Witches’ Broom Diseases. Electrophoresis Journal, 29, 2391-2401. https://doi.org/10.1002/elps.200700743
El Hadrami, I. and Baaziz, M. (1997) Somatic Embryogenesis and Analysis of Peroxydase in Phoenix dactylifera. Biologia Plantarum, 37, 197-203. https://doi.org/10.1007/BF02913210
Baaziz, M., Aissam, F., Brake, Z., Bendiap, K., El Hadrami, I. and Cheick, K. (1994) Electrophoretic Patterns of Acid Soluble Proteins and Active Isoformes of Peroxidase and Polyphenoloxidase Typifying Calli and Somatic Embryos of Two Reputed Date Palm Cultivar in Morocco. Euphytica, 76, 159-168. https://doi.org/10.1007/BF00022160
Van Kammenn, A. and Broumer, D. (1964) Increase of Polyphenoloxidase Activity by a Local Virus Infection in Uninoculated Parts of Leaves. Virology, 22, 9-14. https://doi.org/10.1016/0042-6822(64)90042-X
Leelasuphaku, W., Sivanunsaku, P. and Phongpaichit, S. (2006) Purification, Characterization and Synergistic Activity of β-1,3-Glucanase and Antibiotic Extract from an Antagonistic Bacillus subtilis NSRS 89-24 against Rice Blast and Sheath Blight. Enzyme and Microbe Technology Journal, 38, 990-997. https://doi.org/10.1016/j.enzmictec.2005.08.030
TénéTayo, P.M., Ewané, C.A., Effa, O.P. and Boudjeko, T. (2017) Effects of Chitosan and Snail Shell Powder on Cocoa (Theobroma cacao L.) Growth and Resistance against Black Pod Disease Caused by Phytophthora megakarya. African Journal of Plant Science, 11, 331-340. https://doi.org/10.5897/AJPS2016.1487
TénéTayo, P.M., Dzelamonyuy, A., Omokolo, N.D. and Boudjeko, T. (2019) Enhancement of Theobroma cacao Seedling Growth and Tolerance to Phytophthora megakarya by Heat-Treated Oyster Shell Powder. American Journal of Plant Sciences, 10, 578-594. https://doi.org/10.4236/ajps.2019.104042
Purbajanti, E.D., Slamet, W., Fuskhah, E. and Rosyida (2019) Effects of Organic and Inorganic Fertilizers on Growth, Activity of Nitrate Reductase and Chlorophyll Contents of Peanuts (Arachis hypogaea L.). IOP Conference Series Earth and Environmental Science, 250, Article ID: 012048. https://doi.org/10.1088/1755-1315/250/1/012048
Kulcheski, F.R., Correa, R., Gomes, I.A., de Lima, J.C. and Margis, R. (2015) NPK Macronutrients and microRNA Homeostasis. Frontiers in Plant Science, 6, 451. https://doi.org/10.3389/fpls.2015.00451
Malerba, M. and Cerana, R. (2019) Recent Applications of Chitin- and Chitosan-Based Polymers in Plants. Polymers, 11, 839. https://doi.org/10.3390/polym11050839
Musyimi, D.M., Kahihu, S.W., Buyela, D.K. and Sikuku, P.A. (2005) Allelopathic Effects of Mexican Sunflower [Tithonia diversifolia (Hemsl) A. Gray] on Germination and Growth of Spider Plant (Cleome gynandra L.). Journal of Biodiversity and Environmental Sciences, 2, 26-35.
Goss, M.J., Tubeileh, A. and Goorahoo, D. (2013) A Review of the Use of Organic Amendments and the Risk to Human Health. Advances in Agronomy, 120, 275-379. https://doi.org/10.1016/B978-0-12-407686-0.00005-1
Yuncai, B., Hucs, Z. and Schmidhalt, U. (2008) Effect of Foliar Fertilization Application on the Growth and Mineral Nutrient Content of Maize Seedling under Drought and Salinity. Journal of Botany, 5, 1747-1765.
Bell, R.W. and Dell, B. (2008) Micronutrients for Sustainable Food, Feed, Fibre and Bioenergy Production. IFA, Paris, 1-195.
Bilong, E.G., Ngome, A.F., Abossolo-Angue, M., Madong, B.A., Ndaka, B.S.M. and Bilong, P. (2017) Effets des biomasses vertes de Tithonia diversifolia et des engraisminéraux sur la croissance, le développement et le rendement du manioc (Manihot esculenta Crantz) en zone forestiere du Cameroun. International Journal of Biological and Chemical Science, 11, 1716-1726. http://ajol.info/index.php/ijbcs https://doi.org/10.4314/ijbcs.v11i4.24
Mondal, M.M.A., Malek, M.A., Puteh, A.B., Ismail, M.R., Ashrafuzzaman, M. and Naher, L. (2012) Effect of Foliar Application of Chitosan on Growth and Yield in Okra. Australian Journal of Crop Science, 5, 918-921.
Akter, J., Jannat, R., Hossain, M.M., Ahmed, J.U. and Rubayet, T.M. (2018) Chitosan for Plant Growth Promotion and Disease Suppression against Anthracnose in Chilli. International Journal of Environment, Agriculture and Biotechnology, 3, 806-817. https://doi.org/10.22161/ijeab/3.3.13
Dhakshinamoorthy, S., Mariama, K., Elsen, A. and De Waele, D. (2014) Phenols and Lignin Are Involved in the Defence Response of Banana (Musa) Plants to Radopholus similis Infection. Nematology, 16, 565-576. https://doi.org/10.1163/15685411-00002788
Thakker, J.N., Patel, S. and Dhandhukia, P.C. (2013) Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense. ISRN Biotechnology, 2013, Article ID: 601303. https://doi.org/10.5402/2013/601303
Collingborn, F.M.B., Gowen, S.R. and Mueller-Harvey, I. (2000) Investigations into the Biochemical Basis for Nematode Resistance in Roots of Three Musa Cultivars in Response to Radopholus similis Infection. Journal of Agricultural and Food Chemistry, 48, 5297-301. https://doi.org/10.1021/jf000492z
de Ascensao, A.R.F.D.C. and Dubery, I.A. (2003) Soluble and Wall-Bound Phenolics and Phenolic Polymers in Musa acuminata Roots Exposed to Elicitors from Fusarium oxysporum f. sp. cubense. Phytochemistry, 63, 679-686. https://doi.org/10.1016/S0031-9422(03)00286-3
Wuyts, N., Lognay, G., Verscheure, M., Marlier, M., De Waele, D. and Swennen, R. (2007) Potential Physical and Chemical Barriers to Infection by the Burrowing Nematode Radopholus similis in Roots of Susceptible and Resistant Banana (Musa spp.). Plant Pathology, 56, 878-890. https://doi.org/10.1111/j.1365-3059.2007.01607.x
Ewané, C.A., Lepoivre, P., de Lapeyre de Bellaire, L. and Lassois, L. (2012) Involvement of Phenolic Compounds in the Susceptibility of Bananas to Crown Rot. A Review. Biotechnologie, Agronomie, Société et Environnement, 16, 393-404.
Passardi, F., Cosio, C., Penel, C. and Dunand, C. (2005) Peroxidases Have More Functions than a Swiss Army Knife. Plant Cell Reports, 24, 255-265. https://doi.org/10.1007/s00299-005-0972-6
Mayer, A.M. and Harel, E. (1979) Polyphenol Oxidases in Plants. Phytochemistry, 18, 193-215. https://doi.org/10.1016/0031-9422(79)80057-6
Abeles, F.B., Bosshart, P., Forrence, L.E. and Habiz, W. (1970) Preparation and Purifcation of Glucanase and Chitinase from Bean Leaves. Plant Physiology, 47, 129-134. https://doi.org/10.1104/pp.47.1.129
de Ascensao, A.R.F.D.C. and Dubery, I.A. (2000) Panama Disease: Cell Wall Reinforcement in Banana Roots in Response to Elicitors from Fusarium oxysporum f. sp. cubense Race Four. Phytopathology, 90, 1173-1180. https://doi.org/10.1094/PHYTO.2000.90.10.1173
Pusztahelyi, T. (2018) Chitin and Chitin-Related Compounds in Plant-Fungal Interactions. Mycology, 9, 189-201. https://doi.org/10.1080/21501203.2018.1473299
Pusztahelyi, T., Holb, I.J. and Pócsi, I. (2015) Secondary Metabolites in Fungus-Plant Interactions. Frontiers in Plant Science, 6, 1-23. https://doi.org/10.3389/fpls.2015.00573
Taranto, F., Pasqualone, A., Mangini, G., Tripodi, P., MarilenaMiazzi, M., Pavan, S. and Montemurro, C. (2017) Polyphenol Oxidases in Crops: Biochemical, Physiological and Genetic Aspects. International Journal of Mololecular Science, 18, 377. https://doi.org/10.3390/ijms18020377
Andersen, E.J., Ali, S., Byamukama, E., Yen, Y. and Nepal, M.P. (2018) Disease Resistance Mechanisms in Plants. Genes, 9, 339. https://doi.org/10.3390/genes9070339