Comparison of WeatherMax<sup>®</sup> and Engame<sup>TM</sup> Formulations of Glyphosate on Cotyledon Surface Structure, Chlorophyll A Fluorescence and Shikimate Levels in Isogenic Cotton Cultivars Differing in Roundup Resistance — Oak Academic Publishing
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Comparison of WeatherMax<sup>®</sup> and Engame<sup>TM</sup> Formulations of Glyphosate on Cotyledon Surface Structure, Chlorophyll A Fluorescence and Shikimate Levels in Isogenic Cotton Cultivars Differing in Roundup Resistance
Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
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Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
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Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
1 Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
2 Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
3 Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
The effects of Roundup WeatherMax <sup>®</sup> and Engame<sup>TM</sup> formulations of glyphosate were investigated on the cotyledons of glyphosate resistant (GR) and glyphosate sensitive (GS) isogenic cotton cultivars. Engame<sup>TM</sup> is a mixture of glyphosate and 1-aminomethanamide dihydrogen tetraoxosulfate (AMADS). Fully expanded cotton cotyledons treated with Engame<sup>TM</sup> or AMADS developed surface lesions within 2 hours after treatment whereas surfactant-treated control or WeatherMax <sup>®</sup> -treated tissues did not develop lesions. The Engame<sup>TM</sup> and AMADS damage appeared as depressions which were confirmed by scanning electron microscopy. Light micrographs of cross sections through the depressions revealed collapsed and compressed epidermal and mesophyll cells with congealed cytoplasmic contents in the palisade and spongy mesophyll cells. Changes to photosynthetic electron transport were evident at 4 hours after treatment (HAT) in all treatments as revealed by chlorophyll A fluorescence. In GR cotton, the fluorescence perturbations decreased with time such that at 72 HAT Engame<sup>TM</sup> -treated cotyledons could not be distinguished from the surfactant-or WeatherMax <sup>®</sup> -treated plants. The GS cotton continued to show progressive decreases in the fluorescence parameters Fv/Fm and performance index (PI) to 72 HAT. Shikimate levels increased following glyphosate treatment in glyphosate sensitive cotton and Engame<sup>TM</sup> caused a two-to three-fold greater increase in shikimate compared to WeatherMax <sup>®</sup> . These results indicate that the Engame<sup>TM</sup> -based glyphosate formulation involved structural tissue damage which likely increased glyphosate uptake and subsequently increased inhibition of photosynthesis and the shikimate pathway.
Franz, J.E. (1985) Discovery, Development, and Chemistry of Glyphosate. In: Grossbard, E. and Atkinson, D., Eds., The Herbicide Glyphosate, Butterworths, London, 3-17.
Steinmann, H.H., Dickeduisberg, M. and Theuvsen, L. (2012) Uses and Benefits of Glyphosate in German Arable Farming. Crop Protection, 42, 164-169. https://doi.org/10.1016/j.cropro.2012.06.015
Travlos, I., Cheimona, N. and Bilalis, D. (2017) Glyphosate Efficacy of Different Salt Formulations and Adjuvant Additives on Various Weeds. Agronomy, 7, 60. https://doi.org/10.3390/agronomy7030060
Turner, D.J. (1985) Effects of Glyphosate Performance of Formulation, Additives and Mixing with Other Herbicides. In: Grossbard, E. and Atkinson, D., Eds., The Herbicide Glyphosate, Butterworths, London, 3-17.
Leaper, C. and Holloway, P.J. (2000) Adjuvants and Glyphosate Activity. Pest Management Science, 56, 313-319. https://doi.org/10.1002/(SICI)1526-4998(200004)56:4%3C313::AID-PS147%3E3.0.CO;2-3
De Ruiter, H., Uffing, A.J.M., Meinen, E. and Prins, A. (1990) Influence of Surfactants and Plant Species on Leaf Retention of Spray Solutions. Weed Science, 38, 567-572. https://doi.org/10.1017/S004317450005150X
Jordan, D.L., York, A.C., Griffin, J.L., Clay, P.A., Vidrine, P.R. and Reynolds, D.B. (1997) Influence of Application Variables on Efficacy of Glyphosate. Weed Technology, 11, 354-362. https://doi.org/10.1017/S0890037X00043062
MacIsaac, S.A., Paul, R.N. and Devine. M.D. (1991) A Scanning Electron Microscope Study of Glyphosate Deposits in Relation to Foliar Uptake. Pesticide Science, 31, 53-64. https://doi.org/10.1002/ps.2780310107
Belles, D., Shaner, D., Westra, P. and Brunk, G. (2006) Comparison of Efficacy, Absorption and Translocation of Three Glyphosate Formulations on Velvetleaf (Abutilon thephrasti). Pest Management Science, 62, 1177-1181. https://doi.org/10.1002/ps.1296
De Ruiter, H., Uffing, A.J.M. and Meinen, E. (1996) Influence of Surfactants and Ammonium Sulfate on Glyphosate Phytotoxicity to Quackgrass (Elytrigia repens). Weed Technology, 10, 803-808. https://doi.org/10.1017/S0890037X00040835
Gaskin, R.E. and Holloway P.J. (1992) Some Physiochemical Factors Influencing Foliar Uptake Enhancement of Glyphosate Mono (Isopropylammonium) by Polyoxyethylene Surfactants. Pesticide Science, 34, 195-206. https://doi.org/10.1002/ps.2780340303
/sup>
Cuticle Damage
Shikimate
Uptake
Nalewaja, J.D. and Matysiak, R. (1992) Species Differ in Response to Adjuvants with Glyphosate. Weed Technology, 6, 561-566. https://doi.org/10.1017/S0890037X0003579X
Nalewaja, J.D. and Matysiak, R. (1993) Influence of Diammonium Sulfate and Other Salts on Glyphosate Phytotoxicity. Pesticide Science, 38, 77-84. https://doi.org/10.1002/ps.2780380203
Nalewaja, J.D., Devilliers, B. and Matysiak, R. (1996) Surfactant and Salt Affect Glyphosate Retention and Absorption. Weed Research, 36, 241-247. https://doi.org/10.1111/j.1365-3180.1996.tb01653.x
Laerka, P.E. and Streibig, J.C. (1995) Foliar Absorption of Some Glyphosate Formulations and Their Efficacy on Plants. Pesticide Science, 44, 107-116. https://doi.org/10.1002/ps.2780440202
Kirkwood, R.C., Hetherington, R., Reynolds, T.L. and Marshall, G. (2000) Absorption, Localization, Translocation and Activity of Glyphosate in Barnyardgrass. (Echinochloa crus-galli (L.) Beauv): Influence of Herbicide and Surfactant Concentration. Pest Management Science, 56, 359-367. https://doi.org/10.1002/(SICI)1526-4998(200004)56:4%3C359::AID-PS145%3E3.0.CO;2-S
Suwunnamek, U. and Parker, C. (1975) Control of Cyperus rotundus with Glyphosate: The Influence of Ammonium Sulfate and Other Additives. Weed Research, 15, 13-19. https://doi.org/10.1111/j.1365-3180.1975.tb01090.x
Feng, P.C.C., Ryerse, J.S. and Sammons R.D. (1998) Correlation of Leaf Damage with Uptake and Translocation of Glyphosate in Velvetleaf (Abutilon theophrasti). Weed Technology, 12, 300-307. https://doi.org/10.1017/S0890037X00043852
Feng, P.C.C., Ryerse, J.S., Jones, C. R. and Sammons, R.D. (1999) Analysis of Surfactant Leaf Damage Using Microscopy and Its Relation to Glyphosate or Deuterium Oxide Uptake in Velvetleaf (Abutilon theophrasti). Pesticide Science, 55, 385-386. https://doi.org/10.1002/(SICI)1096-9063(199903)55:3%3C385::AID-PS921%3E3.0.CO;2-9
Feng, P.C.C., Sandbrink, J.J. and Sammons, R.D. (2000) Retention, Uptake and Translocation of 14C-Glyphosate from Track Spryer Applications and Correlation to Rainfastness in Velvetleaf (Abutilon theophrasti). Weed Technology, 14, 127-132. https://doi.org/10.1614/0890-037X(2000)014[0127:RUATOC]2.0.CO;2
Baylis, A.D. (2000) Why Glyphosate Is a Global Herbicide: Strengths, Weaknesses and Prospects. Pest Management Science, 56, 299-308. https://doi.org/10.1002/(SICI)1526-4998(200004)56:4%3C299::AID-PS144%3E3.0.CO;2-K
Young, D.C. (1995) Glyphosate-Sulfuric Acid Adduct Herbicides and Use United States Patent 5,411,944. Union Oil Company of California, Los Angeles, CA.
Percy, K.E. and Baker, E.A. (1987) Effects of Simulated Acid Rain on Production, Morphology and Composition of Epicuticular Wax and on Cuticular Membrane Development. New Phytologist, 107, 577-589. https://doi.org/10.1111/j.1469-8137.1987.tb02928.x
Engame TM (2001) Entek Corp., 6835 Deerpath Road, Elkridge, MD 21075 Engame Technical Bulletin. Platte Chemical Company, Greeley.
Molin, W.T. and Hirase, K. (2004) Comparison of Commercial Glyphosate Formulations for Control of Prickly Sida, Purple Nutsedge, Morningglory and Sickle Pod. Weed Biology and Management, 4, 136-141.
Molin, W.T. and Hirase, K. (2005) Effects of Surfactants and Simulated Rain on the Efficacy of the Engame Formulation of Glyphosate in Johnsongrass, Prickly Sida and Yellow Nutsedge. Weed Biology and Management, 5, 123-127. https://doi.org/10.1111/j.1445-6664.2005.00166.x
Shaner, D.L., Westra P. and Nissen, S. (2006) AMADS Increases the Efficacy of Glyphosate Formulations on Corn. Weed Technology, 20, 179-183.
Bowman, D.T., Gutierrez, O.A., Percy, R.G., Calhoun, D.S. and May, O.L. (2006) Pedigrees of Upland and Pima Cotton Cultivars Released between 1970 and 2005. Mississippi Agricultural & Forestry Experiment Station, Mississippi State, Mississippi.
Shaner, D.L., Nadler-Hassar, T., Henry, W.B. and Koger, C.H. (2005) A Rapid in Vivo Shikimate Accumulation Assay with Excised Leaf Discs. Weed Science, 53, 769-774. https://doi.org/10.1614/WS-05-009R.1
Evans, L.S., Gmur, N.F. and Da Costa, F. (1977) Leaf Surface and Histological Perturbations of Leaves of Phaseolus vulgaris and Helianthus annuus after Exposure to Simulated Acid Rain. American Journal of Botany, 64, 903-913. https://doi.org/10.1002/j.1537-2197.1977.tb11934.x
Haderlie, C., Halderson, J.L., Leino, P.W. and Callihan, R.H. (1989) Chemical Desiccation of Potato Vines. American Potato Journal, 66, 53-62. https://doi.org/10.1007/BF02854424