Phenotypic assessment of breeding population is important to identify robust lines for incorporating into future breeding programs. The objective of this study was to identify potential lines from a wheat ( Triticum aestivum L.) population, based on their morpho-physiological traits, for improved heat tolerance. A subset of 100 lines of the double haploid (DH) population named “Buster”, developed from two successful Oklahoma wheat varieties (Billings and Duster) , w as used in the study. Two experiments were conducted one in a greenhouse and the other in growth chambers. Data on plant height, tiller number, leaf number, and photosynthetic pigments were collected from the greenhouse; whereas the data on physiological parameters (leaf net photosynthesis (Pn), transpiration (T), stomatal conductance (g s ), intercellular carbon dioxide concentration (C i ), electron transport rate (ETR), Photosystem II efficiency (Fv ' /Fm ' ) and instantaneous water use efficiency (IWUE)) were collected from the growth chambers. Buster lines were significantly ( P < 0.05) different both morphologically and physiologically. A wide range of observations among genotypes for different morphological and physiological characteristics was found. For example, the Chlorophyll A:B ratio ranged from 1.8 to 4.3, average plant height ranged from 8.4 to 13.3 cm, and the net photosynthesis under heat stress ranged from 11.29 to 25.28 μmol CO 2 m -2 · s -1 . The differences in leaf physiological parameters were more discernible under heat stress. This study provides a piece of baseline information on morpho-physiological characteristics of Buster lines, and identified lines can be used in future breeding programs for incorporating heat stress tolerance.
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