Plants are regularly exposed to myriads of stress factors that cause tremendous damage to their genetic make-up. To ensure genome stability and survival over several generations under harsher environmental conditions, plants have evolved a unique mechanism for dealing with DNA damage known as the DNA damage response pathway (DDR). It has been proposed that there may exist a relationship between the DNA damage response pathway and abiotic stress response in plants. To further investigate this relationship, we knocked down the soybean suppressor of gamma response 1 gene ( GmSOG1 ), a master regulatory gene of the DDR, in soybean plants and subjected the generated transgenic plants to drought stress analysis. Gene expression analysis of the GmSOG1 gene in drought stressed soybean tissues revealed high levels of expression in buds and young leaves. The root lengths and root fresh weights of transgenic soybean plants grown on Murashige and Skoog media supplemented with Gamborg B5 vitamins (MSB5 media) containing 200 mM mannitol for 10 days were significantly lesser than those of drought stressed wild-type plants. Polyethylene glycol (PEG) induced drought stress assay in vivo resulted in significant damage in transgenic plants compared with wild-type plants. Also, the relative expressions of known drought responsive transcription factors such as GmDREB1 and GmLEA as well as antioxidation related genes like GmAPX and GmCAT were downregulated in transgenic soybean lines relative to wild-type plants. Moreover, wild-type soybean plants accumulated more chlorophyll and less malondialdehyde (MDA) than transgenic lines. A confirmatory experiment in GmSOG1 overexpressing Arabidopsis plants also showed significantly higher survival rates and anti-oxidation enzyme accumulation in drought stressed GmSOG1 overexpressing Arabidopsis lines compared with wild-type plants. These results suggest that the SOG1 gene may play active roles in plant abiotic stress defense.
Goldsmith, P.D. (2008) Economics of Soybean Production, Marketing, and Utilization. In: Johnson, L.P., White, P.A. and Galloway, R., Eds., Soybeans: Chemistry, Production, Processing and Utilization, AOCS Press, Urbana, 117-150. https://doi.org/10.1016/B978-1-893997-64-6.50008-1
Sakai, T. and Kogiso, M. (2008) Soy Isoflavones and Immunity. Journal of Medical Investigation, 55, 167-173. https://doi.org/10.2152/jmi.55.167
Friedman, M. and Brandon, D.L. (2001) Nutritional and Health Benefits of Soy Proteins. Journal of Agriculture and Food Chemistry, 49, 1069-1086. https://doi.org/10.1021/jf0009246
Le, D.T., Nishiyama, R., Watanabe, Y., Tanaka, M., Seki, M., Ham, L., Yamaguchi-Shinozaki, K. Shinozaki, K. and Lam, T. (2012) Differential Gene Expression in Soybean Leaf Tissues at Late Developmental Stages under Drought Stress Revealed by Genome-Wide Transcriptome Analysis. PLoS ONE, 7, e49522. https://doi.org/10.1371/journal.pone.0049522
Hirt, H. and Shinozaki, K. (2004) Plant Responses to Abiotic Stress. Springer, Berlin. https://doi.org/10.1007/b84369
Sinclair, T. and Serraj, R. (1995) Legume Nitrogen-Fixation and Drought. Nature, 378, 344. https://doi.org/10.1038/378344a0
Purcell, L.C. and King, C.A. (1996) Drought and Nitrogen Source Effects on Nitrogen Nutrition, Seed Growth, and Yield in Soybean. Journal of Plant Nutrition, 19, 969-993. https://doi.org/10.1080/01904169609365173
Chen, M., Wang, Q.Y., Cheng, X.G., Xu, Z.S., Li, L.C., Ye, X.G., Xia, L.Q. and Ma, Y.Z. (2007) GmDREB2, a Soybean DRE-Binding Transcription Factor, Conferred Drought and High-Salt Tolerance in Transgenic Plants. Biochemical and Biophysical Research Communications, 353, 299-305. https://doi.org/10.1016/j.bbrc.2006.12.027
Zhang, G., Chen, M., Li, L., Xu, Z., Chen, X., Guo, J. and Ma, Y. (2009) Overexpression of the Soybean GmERF3 Gene, an AP2/ERF Type Transcription Factor for Increased Tolerances to Salt, Drought, and Diseases in Transgenic Tobacco. Journal of Experimental Botany, 60, 3781-3796. https://doi.org/10.1093/jxb/erp214
Gao, S.Q., Chen, M., Xu, Z.S., Zhao, C.P., Li, L., Xu, H.J., Tang, Y.M., Zhao, X. and Ma, Y.Z. (2011) The Soybean GmbZIP1 Transcription Factor Enhances Multiple Abiotic Stress Tolerances in Transgenic Plants. Plant Molecular Biology, 75, 537-553. https://doi.org/10.1007/s11103-011-9738-4
Baxter, A., Mittler, R. and Suzuki, N. (2013) ROS as Key Players in Plant Stress Signaling. Journal of Experimental Botany, 65, 1129-1240. https://doi.org/10.1093/jxb/ert375
Foyer, C.H. and Shigeoka, S. (2011) Understanding Oxidative Stress and Antioxidant Functions to Enhance Photosynthesis. Plant Physiology, 155, 93-100. https://doi.org/10.1104/pp.110.166181
Sancar, A., Lindsey-Boltz, L.A., Unsal-Kacmaz, K. and Linn, S. (2004) Molecular Mechanisms of Mammalian DNA Repair and the DNA Damage Checkpoints. Annual Review of Biochemistry, 73, 39-85. https://doi.org/10.1146/annurev.biochem.73.011303.073723
Nisa, M.U., Huang, Y., Benhamed, M. and Raynaud, C. (2019) The Plant DNA Damage Response: Signaling Pathways Leading to Growth Inhibition and Putative Role in Response to Stress Conditions. Frontiers in Plant Science, 10, 653. https://doi.org/10.3389/fpls.2019.00653
Yoshiyama, K.O., Conklin, P.A., Huefner, N.D. and Britt, A.B. (2009) Suppressor of Gamma Response 1 (SOG1) Encodes a Putative Transcription Factor Governing Multiple Responses to DNA Damage. Proceedings of the National Academy of Sciences, USA, 106, 12843-12848. https://doi.org/10.1073/pnas.0810304106
Yoshiyama, K.O. and Kimura, S. (2018) Ser-Gln Sites of SOG1 Are Rapidly Hyperphosphorylated in Response to DNA Double-Strand Breaks. Plant Signaling & Behavior, 13, e1477904. https://doi.org/10.1080/15592324.2018.1477904
Adachi, S., Minamisawa, K., Okushima, Y., Inagaki, S., Yoshiyama, K., Kondou, Y., Kaminuma, E., Kawashima, M., Toyoda, T., Matsui, M., Kurihara, D., Matsunaga, S. and Umeda, M. (2011) Programmed Induction of Endoreduplication by DNA Double-Strand Breaks in Arabidopsis. Proceedings of the National Academy of Sciences, USA, 108, 10004-10009. https://doi.org/10.1073/pnas.1103584108
Furukawa, T., Curtis, M.J., Tominey, C.M., Duong, Y.H., Wilcox, B.W., Aggoune, D., Hays, J.B. and Britt, A.B. (2010) A Shared DNA-Damage-Response Pathway for Induction of Stem-Cell Death by UVB and by Gamma Irradiation. DNA Repair, 9, 940-948. https://doi.org/10.1016/j.dnarep.2010.06.006
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. and Higgins, D.G. (2007) Clustal W and Clustal X Version 2.0. Bioinformatics, 23, 2947-2948. https://doi.org/10.1093/bioinformatics/btm404
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution, 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
Hinchee, M.A.W., Connor-Ward, D.V., Newell, C.A., McDonnell, R.E., Sato, S.J., Gasser, C.S., Fischhoff, D.A., Re, D.B., Fraley, R.T. and Horsch, R.B. (1988) Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer. Bio-Technology, 6, 915-922. https://doi.org/10.1038/nbt0888-915
Nakagawa, T., Kurose, T., Hino, T., Tanaka, K., Kawamukai, M., Niwa, Y., Toyooka, K., Matsuoka, K., Jinbo, T. and Kimura, T. (2007) Development of Series of Gateway Binary Vectors, pGWBs, for Plant Transformation. Journal of Bioscience and Bioengineering, 104, 34-41. https://doi.org/10.1263/jbb.104.34
Narusaka, M., Shiraishi, T., Iwabuchi, M. and Narusaka, Y. (2010) The Floral Inoculating Protocol: A Simplified Arabidopsis thaliana Transformation Method Modified from Floral Dipping. Plant Biotechnology, 27, 349-351. https://doi.org/10.5511/plantbiotechnology.27.349
Huang, X.S., Luo, T., Fu, X.Z., Fan, Q.J. and Liu, J.H. (2011) Cloning and Molecular Characterization of a Mitogen-Activated Protein Kinase Gene from Poncirus trifoliata Whose Ectopic Expression Confers Dehydration/Drought Tolerance in Transgenic Tobacco. Journal of Experimental Botany, 62, 5191-5206. https://doi.org/10.1093/jxb/err229
Wang, B.Q., Zhang, Q.F., Liu, J.H. and Li, G.H. (2011) Overexpression of PtADC Confers Enhanced Dehydration and Drought Tolerance in Transgenic Tobacco and Tomato: Effect on ROS Elimination. Biochemical and Biophysical Research Communications, 413, 10-16. https://doi.org/10.1016/j.bbrc.2011.08.015
Heath, R.L. and Packer, L. (1968) Photoperoxidation in Isolated Chloroplasts I. Kinetic and Stoichiometry of Fatty Acid Peroxidation. Archives of Biochemistry and Biophysics, 125, 189-198. https://doi.org/10.1016/0003-9861(68)90654-1
Zhang, Z. and Huang, R. (2013) Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis Seedling. Bio-Protocol, 3, e817. https://doi.org/10.21769/BioProtoc.817
Nagano, Y. and Asada, K. (1981) Hydrogen Peroxide Is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts. Plant Cell Physiology, 22, 867-880.
Cakmak, I. and Marschner, H. (1992) Magnesium Deficiency and High Light Intensity on Enhance Activities of Superoxide Dismutase, Peroxidase and Glutathione Reductase in Bean Leaves. Plant Physiology, 98, 1222-1227. https://doi.org/10.1104/pp.98.4.1222
Hu, H., Dai, M., Yao, J., Xiao, B., Li, X., Zhang, Q. and Xiong, L. (2006) Overexpressing a NAM, ATAF, and CUC (NAC) Transcription Factor Enhances Drought Resistance and Salt Tolerance in Rice. Proceedings of the National Academy of Sciences, USA, 103, 12987-12992. https://doi.org/10.1073/pnas.0604882103
Liu, F., Anderson, M.N., Jacobson, S.E. and Jensen, C.R. (2005a) Stomatal Control and Water Use Efficiency of Soybean (Glycine max L. Merr.) during Progressive Soil Drying. Environmental and Experimental Botany, 54, 33-40. https://doi.org/10.1016/j.envexpbot.2004.05.002
Benjamin, J.G. and Nielsen, D.C. (2006) Water Deficit Effects on Root Distribution of Soybean, Field Pea and Chickpea. Field Crops Research, 97, 248-253. https://doi.org/10.1016/j.fcr.2005.10.005
Liu, Y., Gai, J.Y., Lu, H.N., Wang, Y.J. and Chen, S.Y. (2005) Identification of Drought Tolerant Germplasm and Inheritance and QTL Mapping of Related Root Traits in Soybean (Glycine max (L.) Merr.). Acta Genetica Sinica, 32, 855-863.
Read, D.J. and Bartlett, E.M. (1972) The Physiology of Drought Resistance in Soybean Plant (Glycine max): The Relationship between Drought Resistance and Growth. Journal of Applied Ecology, 9, 487-489. https://doi.org/10.2307/2402447
Lopes, M.S., Araus, J.L., Van Heerden, P.D.R. and Foyer, C.H. (2011) Enhancing Drought Tolerance in C4 Crops. Journal of Experimental Botany, 62, 3135-3153. https://doi.org/10.1093/jxb/err105
De Ronde, J.A., Spreeth, M.H. and Cress, W.A. (2000) Effect of Antisense L-Δ1-Pyrroline-5-Carboxylate Reductase Transgenic Soybean Plants Subjected to Osmotic and Drought Stress. Plant Growth Regulation, 32, 13-26. https://doi.org/10.1023/A:1006338911617
Ogita, N., Okushima, Y., Tokizawa, M., Yamamoto, Y.Y., Tanaka, M., Seki, M., Makita, Y., Matsui, M., Okamoto-Yoshiyama, K., Sakamoto, T., Kurata, T., Hiruma, K., Saijo, Y., Takahashi, N. and Umeda, M. (2018) Identifying the Target Genes of Suppressor of Gamma Response 1, a Master Transcription Factor Controlling DNA Damage Response in Arabidopsis. The Plant Journal, 94, 439-453. https://doi.org/10.1111/tpj.13866
Cushman, J.C. and Bohnert, H.J. (2000) Genomic Approaches to Plant Stress Tolerance. Current Opinion Plant Biology, 31, 117-124. https://doi.org/10.1016/S1369-5266(99)00052-7
Foyer, C.H. and Noctor, G. (2003) Redox Sensing and Signalling Associated with Reactive Oxygen in Chloroplasts, Peroxisomes and Mitochondria. Physiological Plantarum, 119, 355-364. https://doi.org/10.1034/j.1399-3054.2003.00223.x
Phang, T., Shao, G. and Lam, H. (2008) Salt Tolerance in Soybean. Journal of Integrative Plant Biology, 50, 1196-1212. https://doi.org/10.1111/j.1744-7909.2008.00760.x
Yoshiyama, K.O., Kimura, S., Maki, H., Britt, A.B. and Umeda, M. (2014) The Role of SOG1, a Plant-Specific Transcriptional Regulator, in the DNA Damage Response. Plant Signaling & Behavior, 9, e28889. https://doi.org/10.4161/psb.28889
Sajedi, N.A., Ardakani, M.R., Rejali, F., Mohabbati, F. and Miransari, M. (2010) Yield and Yield Components of Hybrid Corn (Zea mays L.) as Affected by Mycorrhizal Symbiosis and Zinc Sulfate under Drought Stress. Physiology and Molecular Biology of Plants, 16, 343-351. https://doi.org/10.1007/s12298-010-0035-5
Sajedi, N.A., Ardakani, M.R., Madani, H., Naderi, A. and Miransari, M. (2011) The Effects of Selenium and Other Micronutrients on the Antioxidant Activities and Yield of Corn (Zea mays L.) under Drought Stress. Physiology and Molecular Biology of Plants, 17, 215-222. https://doi.org/10.1007/s12298-011-0067-5