Antimicrobial and Antioxidant Activities of Methanolic Extract and Fractions of <i>Epilobium roseum</i> (Schreb.) against Bacterial Strains — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Antimicrobial and Antioxidant Activities of Methanolic Extract and Fractions of <i>Epilobium roseum</i> (Schreb.) against Bacterial Strains
Department of Botany, Hazara University, Mansehra, Pakistan
,
Department of Botany, Hazara University, Mansehra, Pakistan
,
Department of Botany, Abdul Wali Khan University Mardan, Pakistan
,
Department of Botany, Abdul Wali Khan University Mardan, Pakistan
,
Department of Botany, University of Chitral, Chitral, Pakistan
,
Department of Botany, Hazara University, Mansehra, Pakistan
,
Department of Botany, Hazara University, Mansehra, Pakistan
,
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
1 Department of Botany, Hazara University, Mansehra, Pakistan
2 Department of Botany, Hazara University, Mansehra, Pakistan
3 Department of Botany, Abdul Wali Khan University Mardan, Pakistan
4 Department of Botany, Abdul Wali Khan University Mardan, Pakistan
5 Department of Botany, University of Chitral, Chitral, Pakistan
6 Department of Botany, Hazara University, Mansehra, Pakistan
7 Department of Botany, Hazara University, Mansehra, Pakistan
8 Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Present study aims to evaluate antimicrobial and antioxidant activities of the crude methanolic extract and different fractions of the Epilobium roseum (Schreb). The extract and fractions were used against pathogenic bacteria ( Bacillus subtilis , Bacillus atrophaeus, Klebsiella pneumonia and Pseudomonas aeruginosa ) and fungal strains ( Aspergillus niger and Aspergillus flavus ). The methanolic extract and their sub fraction n-hexane showed a prominent inhibition zone against all bacterial strains but inactive against fungal strains. The various extracts of Epilobium roseum (Schreb) from various parts were tested for their antioxidant activity by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay . The IC 50 of the stem and root of methanolic extract and their sub fraction n-hexane showed best activity ranged between 22.73 ± 6.92, 21.49 ± 6.26 and 14.94 ± 3.54, 13.92 ± 1.04 μg/ml compared to another fraction. The results support that Epilobium roseum can be used as antimicrobial and antioxidant agents. The results support the present study that Epilobium roseum (Schreb) has a potential source of natural antibacterial, antifungal and antioxidant potentials.
Prasad, K.N., Divakar, S., Shivamurthy, G.R. and Aradhya, S.M. (2005) Isolation of a Free Radical-Scavenging Antioxidant from Water Spinach (Ipomoea aquatica Forsk). Journal of the Science of Food and Agriculture, 85, 1461-1468. https://doi.org/10.1002/jsfa.2125
Calixto, J.B. (2000) Efficacy, Safety, Quality Control Marketing and Regulatory Guidelines for Herbal Medicines (Phytotherapeutic agents). Brazilian Journal of Medical and Biological Research, 33, 179-189. https://doi.org/10.1590/S0100-879X2000000200004
Khan, N. and Bano, A. (2016) Role of Plant Growth Promoting Rhizobacteria and Ag-Nano Particle in the Bioremediation of Heavy Metals and Maize Growth Under Municipal Wastewater Irrigation. International Journal of Phytoremediation, 18, 211-221. https://doi.org/10.1080/15226514.2015.1064352
Ifesan, B.O.T., Siripongvutikorn, S., Hutadilok-Towatana, N. and Voravuthikuncha, S.P. (2009) Evaluation of the Ability of Eleutherine americana Crude Extract as Natural Food Additive in Cooked Pork. Journal of Food Science, 74, 352-357. https://doi.org/10.1111/j.1750-3841.2009.01254.x
Ahn, J., Grun, I.U. and Mustapha, A. (2007) Effects of Plant Extracts on Microbial Growth, Colour Change and Lipid Oxidation in Cooked Beef. Food Microbiology, 24, 7-14. https://doi.org/10.1016/j.fm.2006.04.006
Jagessar, R.C., Mohamed, A. and Gomes, G. (2008) An Evaluation of the Antibacterial and Antifungal Activity of Leaf Extracts of Momordica Charantia against Candida Albicans, Staphylococcus aureus and Escherichia coli. Nature and Science, 6, 1545-1570.
Kousar, B., Bano, A. and Khan, N. (2020) PGPR Modulation of Secondary Metabolites in Tomato Infested with Spodoptera Litura. Agronomy, 10, 778. https://doi.org/10.3390/agronomy10060778
Bodeker, G. and Kronenberg, F. (2002) A Public Health Agenda for Traditional, Complementary and Alternative Medicine. American Journal of Public Health, 92, 1582-1591. https://doi.org/10.2105/AJPH.92.10.1582
Mann, A., Banso, A. and Clifford, I. (2008) An Antifungal Property of Crude Plant Extracts from Anogeissus leiocarpus and Terminalia avicennioides. Tanzania Journal of Health Research, 10, 34-38. https://doi.org/10.4314/thrb.v10i1.14339
Joshi, B., Lekhak, S. and Sharma, A. (2009) Antibacterial Property of Different Medicinal Plants: Ocimum sanctum, Cinnamomum zeylanicum, Xanthoxylum armatum and Origanum majorana. Kathmandu University Journal of Science, Engineering and Technology, 5, 143-150. https://doi.org/10.3126/kuset.v5i1.2854
Bajpai, M.A., Pande, S. and Prakash, D. (2005) Phenolic Contents and Antioxidant Activity of Some Food and Medicinal Plants. International Journal of Food Sciences and Nutrition, 56, 287-291. https://doi.org/10.1080/09637480500146606
Mothana, R.A. and Lindequist, U. (2005) Antimicrobial Activity of Some Medicinal Plants of the Island Soqotra. Journal of Ethnopharmacology, 96, 177-181. https://doi.org/10.1016/j.jep.2004.09.006
Mostafa, A.A., Al-Askar, A.A., Almaary, K.S., Dawoud, T.M., Sholkamy, E.N. and Bakri, M.M. (2018) Antimicrobial Activity of Some Plant Extracts against Bacterial Strains Causing Food Poisoning Diseases. Saudi Journal of Biological Sciences, 25, 361-366. https://doi.org/10.1016/j.sjbs.2017.02.004
Tekwu, E.M., Pieme, A.C. and Beng, V.P. (2012) Investigations of Antimicrobial Activity of Some Cameroonian Medicinal Plant Extracts against Bacteria and Yeast with Gastrointestinal Relevance. Journal of Ethnopharmacology, 142, 265-273. https://doi.org/10.1016/j.jep.2012.05.005
Manandhar, S., Luitel, S. and Dahal, R.K. (2019) In Vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria. Journal of Tropical Medicine, 2019, 1-5. https://doi.org/10.1155/2019/1895340
Chevolleau, S., Mallet, J.F., Debal, A. and Ucciani, E. (1993) Antioxidant Activity of Mediterranean Plant Leaves: Occurrence and Antioxidative Importance of α-Tocopherol. Journal of the American Oil Chemists’ Society, 70, 807-809. https://doi.org/10.1007/BF02542606
Rahman, A., Khan, R.M., Choudhary, I.M. and Iqbal, Z.M. (1997) Steroidal Alkaloids from Sarcococca saligna. Phytochemistry, 45, 861-864. https://doi.org/10.1016/S0031-9422(97)00069-1
Safarzadeh, E., Shotorbani, S.S. and Baradaran, B. (2014) Herbal Medicine as Inducers of Apoptosis in Cancer Treatment. Advanced Pharmaceutical Bulletin, 4, 421-427.
Chevallier, A. (1996) The Encyclopedia of Medicinal Plants. DK Publishing Inc., New York, 11-93.
Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z. and Ercisli, S. (2009) Total Phenolics Content, Antioxidant and Antimicrobial Activities of Some Medicinal Plants. Pakistan Journal of Pharmaceutical Sciences, 22, 102-106.
Nisar, M., Kaleem, W.A., Qayum, M., Marwat, I.K., Zia-ul-Haq, M., Ali, I. and Choudhary, M. (2011) Biological Screening of Zizyphus oxyphylla Edgew Stem. Pakistan Journal of Botany, 43, 311-317.
Chowdhary, S., Harish, K. and Verma, D.L. (2009) Chemical Examination of Bergenia stracheyi (HK) for Antioxidative Flavonoids. Nature and Science, 7, 29-34.
Pourmorad, F., Hosseinimehr, S.J. and Shahabimajd, N. (2006) Antioxdant Activity, Phenol and Flavonoid Contents of Some Selected Iranian Medicinal Plants. African Journal of Biotechnology, 11, 1142-1145.
Veeru, P., Kishar, M. and Meenakshi, M. (2009) Screening of Medicinal Plant Extracts for Antioxidant Activity. Journal of Medicinal Plants Research, 3, 608-612.
Mensor, L.L., Menezes, F.S., Leitao, G.G., Reis, A.S. and Santos, T.C. (2001) Screening of Brazilian Plant Extracts for Antioxidant Activity by the Use of DPPH Free Radical Method. Phytotherapy Research, 15, 127-130. https://doi.org/10.1002/ptr.687
Killedar, S.G. and More, H.N. (2011) Screening of Antimicrobial Potential and Phytoconsituents for Different Extracts of Memecylon umbellatum Burm Inflorescences. Asian Journal of Pharmaceutical Research, 1, 114-118.
Koleva, I.I., van Beek, T.A, Linssen, J.P.H., Groot, A.D. and Evstatieva, L.N. (2002) Screening of Plant Extracts for Antioxidant Activity: A Comparative Study on Three Testing Methods. Phytochemical Analysis, 13, 8-17. https://doi.org/10.1002/pca.611
Parekh, J., Karathia, N. and Chanda, S. (2006) Screening of Some Traditionally Used Medicinal Plants for Potential Antibacterial Activity. Indian Journal of Pharmaceutical Sciences, 68, 832-834. https://doi.org/10.4103/0250-474X.31031
Maiyo, Z.C., Ngure, R.M. and Matasyo, J.C. (2010) Phytochemical Constituents and Antimicrobial Activity of Leaf Extracts of Three Amaranthus Plant Species. African Journal of Biotechnology, 9, 3178-3182.
Dai, J., Zhu, L., Yang, L. and Qiu, J. (2013) Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil from Wedelia prostrata. EXCLI Journal, 12, 479-490.
Wong, S.P., Leong, L.P. and Koh, J.H.W. (2006) Antioxidant Activities of Aqueousextracts of Selected Plants. Food Chemistry, 99, 775-783. https://doi.org/10.1016/j.foodchem.2005.07.058
Khan, N., Bano, A. and Zandi, P. (2018) Effects of Exogenously Applied Plant Growth Regulators in Combination with PGPR on the Physiology and Root Growth of Chickpea (Cicer arietinum) and Their Role in Drought Tolerance. Journal of Plant Interactions, 13, 239-247. https://doi.org/10.1080/17429145.2018.1471527
Khan, N. and Bano, A. (2019) Exopolysaccharide Producing Rhizobacteria and Their Impact on Growth and Drought Tolerance of Wheat Grown under Rainfed Conditions. PLoS ONE, 14, e0222302. https://doi.org/10.1371/journal.pone.0222302
Oroian, M. and Escriche, I. (2015) Antioxidants: Characterization, Natural Sources, Extraction and Analysis. Food Research International, 74, 10-36. https://doi.org/10.1016/j.foodres.2015.04.018
Khan, N., Bano, A. and Curá, J.A. (2020) Role of Beneficial Microorganisms and Salicylic Acid in Improving Rainfed Agriculture and Future Food Safety. Microorganisms, 8, 1018. https://doi.org/10.3390/microorganisms8071018
Naseem, H., Ahsan, M., Shahid, M.A. and Khan, N. (2018) Exopolysaccharides Producing Rhizobacteria and Their Role in Plant Growth and Drought Tolerance. Journal of Basic Microbiology, 58, 1009-1022. https://doi.org/10.1002/jobm.201800309
Wickens, A.P. (2001) Ageing and the Free Radical Theory. Respiration Physiology, 128, 379-391. https://doi.org/10.1016/S0034-5687(01)00313-9
Tilak, J.C., Adhikari, S. and Devasagayam, T.P.A. (2004) Antioxidant Properties of Plumbago zeylanica, and Indian Medicinal Plant and Its Active Ingredient, Plumbagin. Redox Report, 9, 219-227. https://doi.org/10.1179/135100004225005976
Havasi, C., Speer, R., Pustejovsky, J., et al. (2009) Digital Intuition: Applying Common Sense Using Dimensionality Reduction. IEEE Intelligent Systems, 24, 24-35. https://doi.org/10.1109/MIS.2009.72