Acetone Extract of <i>Dioscorea alata</i> Inhibits Cell Proliferation in Cancer Cells

Abstract

Dioscorea spp., White Yam has been shown to exhibit a wide range of nutritional and medicinal properties. However, the compounds associated with its medicinal functions have not been fully examined. The purpose of this study was to generate a chemoinformatic profile of the bioactive compounds present in Dioscorea . alata ( D . alata ) and to characterize their putative anti-cancer properties using prostate (DU145) and lung (A549) cancer cells. Chemoinformatic profiling of D . alata resulted in five bioactive extracts : hexane (DaJa-1), ether (DaJa-2), acetone (DaJa-3), ethanol (DaJa-4) and water (DaJa-5) were generated. The analytes present in the five bioactive extracts were dissolved in 0.1% DMSO and their anti-cancer activity were determined. We observed that the acetone extract (DaJa3) was the only extract capable of inducing greater tha n 90% cell death of DU 145 cell at 100 μg/mL. The order of growth inhibition of the extracts in DU-145 cell is DaJa-3 (IC50, 31.45 μg/mL) > DaJa-4 (IC50 60 μg/mL) > DaJa-1 (IC50 > 100 μg/mL) ≥ DaJa-2 (IC50 > 100 μg/mL) ≥ DaJa-5 (IC50 > 100 μg/mL). MTT cell viability, dye exclusion, caspase activity and microscopic assessment of apoptotic cells demonstrated that DaJa-3 displayed cytotoxicity to both lung and prostate cancer cells. The A549 cells were more sensitive toward DaJa-3 with an IC 50 value of 22.28 μg/mL (CI 28.42 to 36.63 μg/mL), compared to that of DU145 cells with an IC 50 value of 31.45 μg/mL (CI 27.58 to 35.86 μg/mL). It was also observed that DaJa-3 induces differential anti-proliferative activity in the cancer cells. The apoptotic response induced by DaJa-3 paralleled the level of cell cytotoxicity observed in both cell lines. DaJa-3 induces G 2 phase cell cycle arrest in DU145 cells but G 1 arrest in A549 cells. The level of key apoptotic regulator proteins was upregulated, suggest ed that DaJa-3 may be mediating its anti-cancer effect through activation of the intrinsic apoptotic pathway. Altogether, our data indicate s that DaJa-3 derived from a staple food source (white Yam) contains unique active compounds that have specific biological properties that may prevent certain types of cancer or specific types of cancer.