Genetic diversity in 30 genotypes of Pumpkins was collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00 - 0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity; to broaden the gene pool. Further collection of the important germplasm is needed to be used in the development of improved cultivars with respect to quality and quantity.
Ghafoor, A., Sharif, A., Ahmad, Z., Aahid, M.A. and Rabbani, M.A. (2001) Genetic Diversity in Blackgram (Vigna mungo L. Hepper). Field Crops Research, 69, 183-190. https://doi.org/10.1016/S0378-4290(00)00141-6
Akhtar, M. (2001) Phylogenetic Relationship among Vigna Species Based on Agronomic and Biochemical Analysis. M. Phil. Thesis, Quaid-I-Azam University, Islamabad, 99 p.
Javid, I., Ghafoor, A. and Anwar, R. (2004) Seed Storage Protein Electrophoresis in Groundnut for Evaluating Genetic Diversity. Pakistan Journal of Botany, 36, 25-29.
Al-Khalifah, N.S., Askari, E. and Khan, A.S. (2012) Molecular and Morphological Identification of Some Elite Varieties of Date-Palms Grown in Saudi Arabia. Emirates Journal of Food and Agriculture, 24, 456-461.
Ullah, I., Ahmad, K.I., Ahmad, H., Gafoor, S.G., Muhammad, I. and Ilyas, M.S. (2010) Storage Protein Profile of Rice Varieties Commonly Grown in Pakistan. Asian Journal of Agricultural Sciences, 2, 120-123.
Arshad, M.A., Bakhsh, K., Zubair, M. and Ghafoor, A. (2003) Genetic Variability and Correlation Studies in Chickpea (Cicer arietinum L.). Pakistan Journal of Botany, 35, 605-611.
Ashraf, M., Quresh, A.S. and Ghafoor, A. (2003) Genetic Diversity in Wheat under Different Crop Ecological Zones. Pakistan Journal of Botany, 35, 597-603.
Bharawadraj, C., Satyavath, C.T. and Subramanyam, D. (2001) Evaluation of Different Classificatory Analysis Methods in Some Rice (Oryza sativa L.) Collections. The Indian Journal of Agricultural Sciences, 71, 123-125.
Doijode, S.D. and Sulladmath, U.V. (1986) Genetic Variability and Correlation Studies in Pumpkin (Cucurbita moschata Poir.). Mysore Journal of Agricultural Sciences, 29, 59-61.
Sultana, T., Ghafoor, A. and Ashraf, M. (2005) Genetic Divergence in Lentil Germplasm for Botanical Descriptors in Relation with Geographic Origin. Pakistan Journal of Botany, 37, 61-69.
Malik, M.F.A., Qureshi, A.S., Ashraf, M., Khan, M.R. and Javed, A. (2009) Evaluation of Genetic Diversity in Soybean (Glycine max) Lines Using Seed Protein Electrophoresis. Australian Journal of Crop Science, 3, 107-112.
Mahfouze, S.A. and Ottai, M.E. (2011) Assessment of Genetic Variability for Some Hyoscymus Species Using Biochemical and Molecular Markers. Journal of Applied Sciences Research, 7, 1752-1759.
Ghafoor, A., Ahmad, Z., Qureshi, A.S. and Bashir, M. (2002) Genetic Relationship in Vigna mungo L. Hepper and V. radiate (L.) R. Wilczek Based on Morphological Traits and SDS-PAGE. Euphytica, 123, 367-378. https://doi.org/10.1023/A:1015092502466
Akasha, I., Campbell, L., Lonchamp, J. and Euston, S.R. (2016) The Major Protein of Seed of Fruit of Date Palm (Pheonix dactylifera L.): Characterization and Emulsifying Properties. Food Chemistry, 197, 799-806. https://doi.org/10.1016/j.foodchem.2015.11.046
Hu, S.W., Ovesna, J., Kucera, l., Kucera, V. and Vyvadilova, M. (2003) Evaluation of Genetic Diversity of Brassica napus Germplasm from China and Europe Assessed by RAPD Markers. Plant, Soil and Environment, 49, 106-113. https://doi.org/10.17221/4098-PSE
Ravi, M., Geethanjali, S., Sameeyafarheen, F. and Maheswaran, M. (2003) Molecular Marker Based Genetic Diversity Analysis in Rice (Oryza sativa L.) Using RAPD and SSR Markers. Euphytica, 133, 243-252. https://doi.org/10.1023/A:1025513111279
Gopalakrishnan, T.R., Gopalakrishnan, K.G. and Peter, K.V. (1980) Variability, Heritability and Correlation among Some Polygenic Characters in Pumpkin. The Indian Journal of Agricultural Sciences, 50, 925-930.
Horejsi, T. and Stub, J.E. (1999) Genetic Variation in Cucumber (Cucumis sativus L.) as Assed by Random Amplified Polymorphic DNA. Genetic Resources and Crop Evolution, 46, 337-350. https://doi.org/10.1023/A:1008650509966
Javid, A., Ghafoor, A. and Anwar, R. (2004) Seed Storage Protein Electrophoresis in Groundnut for Evaluating Genetic Diversity. Pakistan Journal of Botany, 36, 87-96.
Gwanama, C., Labuschagne, M.T. and Botha, A.M. (2000) Analysis of Genetic Variation in Cucurbita moschata by Random Amplified Polymorphic DNA (RAPD) Markers. Euphytica, 113, 19-24. https://doi.org/10.1023/A:1003936019095
Kashif, M. and Khan, F.A. (2007) Divergence in Sugarcane (Saccharum officinarum) Based on Yield and Quality Traits. Pak. J. Bot., 39, 1559-1563.
Williams, J.G.K., Kubelik, A.R., Livik, K.J., Rafalski, J.A. and Tingery, S.V. (1990) DNA Polymorphisms Amplified Arbitrary Primers Are Useful as Genetic Markers. Nucleic Acids Research, 18, 6531-6535. https://doi.org/10.1093/nar/18.22.6531
Iqbal, S.H., Ghafoor, A. and Ayub, N. (2005) Relationship between SDS-PAGE Markers and Ascochyta Blight in Chickpea. Pakistan Journal of Botany, 37, 87-96.
Isemura, T., Noda, C., Mori, S., et al. (2001) Genetic Variation and Geographical Distribution of Azuki Bean (Vigna angularis) Landraces Based on the Electrophoregram of Seed Storage Proteins. Breeding Science, 51, 225-230. https://doi.org/10.1270/jsbbs.51.225
Jehan, B., Fareed, A., Shafi, M. and Swati, A.Z. (2011) Genetic Diversity of Nuryt-National Uniform Rape Seed Yield Trial and Brassica napus Varieties Using RAPD Markers and Biochemical Analysis. Pakistan Journal of Botany, 43, 2075-2081.
Lakshmi, L.M., Haribabu, K. and Reddy, G.L.K. (2002) Genetic Variability Studies in Pumpkin (Cucurbita moschata Duch ex. poir). The Journal of Research, ANGRAU, 30, 82-86.
Khoshroo, S.M.R., Khavarinejad, R., Baghizadeh, A., Fahimi, H. and Mohammadi, Z.N. (2012) Seed Storage Protein Electrophoretic Profiles in Some Iranian Date Palm (Phoenix dactylifera L.) Cultivars. African Journal of Biotechnology, 10, 17793-17804. https://doi.org/10.5897/AJB11.2726
Sadeghi, Z. and Kuhestani, K. (2014) Ethnobotany of Date Palm (Phoenix dactylifera) in Baluch Tribe of Saravan Region, Baluchistan, Iran. International Journal of Agricultural Technology, 10, 1571-1585.
Rabbani, M.A., Qureshi, A., Afzal, M., Anwar, R. and Komatsu, S. (2001) Characterization of Mustard [Brassica junncea (L.) Cezern. & Cross] Germplasm by SDS-PAGE of Total Seed Protein. Pakistan Journal of Botany, 33, 173-179.
Lee, H.X., Ahmad, F., Saad, B. and Ismail, M.N. (2017) Evaluation of Extraction Methods for the Identification of Proteins from Date Palm (Phoenix dactylifera L.) Seed and Flesh. Preparative Biochemistry & Biotechnology, 47, 998-1007. https://doi.org/10.1080/10826068.2017.1365250
Parker, P.G., Snow, A.A., Schug, M.D., Booton, G.C. and Fuerst, P.A. (1998) What Molecules Can Tell Us about Populations: Choosing and Using a Molecular Markers. Ecology, 79, 361-382. https://doi.org/10.1890/0012-9658(1998)079[0361:WMCTUA]2.0.CO;2
Shafique, S., Khan, M.R., Nisar, M. and Rehman, S. (2011) Investigation of Genetic Diversity in Black Gram [Vigna mungo (L.) hepper]. Pakistan Journal of Botany, 43, 1223-1232.
Netra, N. and Prasad, S. (2007) Identification of Rice Hybrids and Their Parental Lines Based on Seed, Seedling Characters, Chemical Tests and Gel Electrophoresis of Total Soluble Seed Proteins. Seed Science and Technology, 35, 176-186. https://doi.org/10.15258/sst.2007.35.1.16
Rafalski, J.A. and Tingey, S.V. (1993) Genetic Diagnosis in Plant Breeding: RAPDs Microsatellite and Machines. Trends in Genetics, 9, 275-280. https://doi.org/10.1016/0168-9525(93)90013-8
Khan, N. and Ali, S. (2017) Advances in the Detection of Genetic Diversity in Bread Wheat. American Journal of Agricultural Science, 4, 29-36.
Riggs, D.I., et al. (2003) Pumpkin Production Guide. NRAES-123. Northeast Regional Agric. Engineering Service, Ithaca, 152 p.
Zahir, A.S., Qureshi. Waqal, A., Haseena, G., Mohammad, N. and Ghafoor, A. (2007) Evaluation of Genetic Diversity Present in Pea (Pisum sativum L.) Germplasm Based on Morphological Traits, Resistance to Powdery Mildew and Molecular Characteristics. Pakistan Journal of Botany, 39, 2739-2747.
Sadia, M., Malik, S.A. Rabbani, M.A. and Peaece, S.R. (2009) Electrphoretic Characterization and the Relationship between Some Brassica Species. Electronic Journal of Biology, 5, 1-4.
Singh, N.P. and Matta, N.K. (2008) Variation Studies on Seed Storage Proteins and Phylogenetics of the Genus Cucumis. Plant Systematics and Evolution, 275, Article No. 209. https://doi.org/10.1007/s00606-008-0063-6
Ibrahim, S.M., Ramalingam, A. and Subramanian, M. (1990) Using Metroglyph Analysis to Study Variability in Early Generations of Rainfed Lowland Rice. International Rice Research Newsletter, 15, 8-9.
Mehrani, P. (2002) Genetic Diversity in Local and Exotic Pea (Pisaum sativum L.) Germplasm for Morphological Traits and SDS-PAGE Markers. M. Phil. Thesis, Quaid-I-Azam University, Islamabad, 104 p.
Ghafoor, A. and Ahmad, Z. (2005) Diversity in Black Gram [Vigna mungo (L.) Hepper] for Agronomic Traits and Total Seed Proteins Analysis. Acta Biologica Cracoviensia Series Botanica, 47, 1-7.
Sharma, B.I., Singh, D.P. and Ram H.H. (1996) Genetic Divergence in Green Gram Germplasm through Use of Non-Hierarchal Euclidean Analysis. The Indian Journal of Agricultural Sciences, 66, 193-196.
Mohammad, A.A. (2015) Electrophoretic Analysis of Proteins from Different Date Palm (Phoenix dactylifera L.) Cultivars in Saudi Arabia. African Journal of Biotechnology, 14, 1325-1333. https://doi.org/10.5897/AJB2014.14398
Mohammad, N., Naeem, K., Zakia, A. and Abdul, G. (2011) Genetic Diversity and Disease Response of Rust in Bread Wheat Collected from Waziristan Agency, Pakistan. International Journal of Biodiversity and Conservation, 3, 10-18.