Heterogeneous Expression and Purification of the Wheat VRN1 K-Box Domain Suggest the Formation of a Tetramer of the VRN1 Protein — Oak Academic Publishing
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Heterogeneous Expression and Purification of the Wheat VRN1 K-Box Domain Suggest the Formation of a Tetramer of the VRN1 Protein
Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan
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Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan
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Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan
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Department of Sustainable Agri-Culture, Fukui Prefectural University, Fukui, Japan
1 Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan
2 Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan
3 Faculty of Bioscience and Biotechnology, Fukui Prefectural University, Fukui, Japan
4 Department of Sustainable Agri-Culture, Fukui Prefectural University, Fukui, Japan
In cereal species such as wheat ( Ttiticum aestivum ) and barley ( Hordeum vulg are ), many studies have indicated that VERNALIZATION 1 ( VRN 1) functions as a flowering promoter, which activates florigen gene expression. The wheat florigen gene Wheat FLOWERING LOCUS T ( WFT , which is identical to VRN 3) is an integrator of the vernalization, photoperiod and au tonomous pathways in wheat flowering, and the WFT expression is corre lated with the V RN 1 expression. VRN 1 encodes an APETALA1/FRUITFULL-lik e MADS-box transcription factor which expression is induced by vernalization, leading to flowering thorough up-regulation of WFT . In Arabidopsis , it has been reported that protein-protein interactions are keys for MADS-box protein function and MADS-box transcription factors must dimerize to bind to the target gene. In this study, by using gel permeation chromatography (GPC) with purified VRN1 protein, we indicated the possibility that VRN1 protein exists as tetramer-like as flowering homeotic MADS-box proteins in Arabidopsis .
Kim, D.H., Doyle, M.R., Sung, S. and Amasino, R.M. (2009) Vernalization: Winter and the Timing of Flowering in Plants. Annual Review of Cell Developmental Biology, 25, 277-299. https://doi.org/10.1146/annurev.cellbio.042308.113411
Ream, T.S., Woods, D.P. and Amasino, R.M. (2013) The Molecular Basis of Vernalization in Different Plant Groups. Cold Spring Harbor Symposium of Quantitative Biology 2012, 77, 105-115. https://doi.org/10.1101/sqb.2013.77.014449
Travaskis, B., Hemming, M.N., Dennis, E.S. and Peacock, W.J. (2007) The Molecular Basis of Vernalization-Induced Flowering in Cereals. Trends in Plant Science, 12, 352-357. https://doi.org/10.1016/j.tplants.2007.06.010
Danyluk, J., Kane, N.A., Breton, G., Limin, A.E., Fowler, D.B. and Sarhan, F. (2003) TaVRT-1, a Putative Transcription Factor Associated with Vegetative to Reproductive Transition in Cereals. Plant Physiology, 132, 1849-1860. https://doi.org/10.1104/pp.103.023523
Murai, K., Miyamae, M., Kato, H., Takumi, S. and Ogihara, Y. (2003) WAP1, a Wheat APETALA1 Homolog, Plays a Central Role in the Phase Transition from Vegetative to Reproductive Growth. Plant and Cell Physiology, 44, 1255-1265. https://doi.org/10.1093/pcp/pcg171
Trevaskis, B., Bagnall, D.J., Ellis, M.H., Peacock, W.J. and Dennis, E.S. (2003) MADS Box Genes Control Vernalization-Induced Flowering in Cereals. Proceedings of the National Academy of Sciences of the United States of America, 100, 13099-13104. https://doi.org/10.1073/pnas.1635053100
Yan, L., Loukoianov, A., Tranquilli, G., Helguera, M., Fahima, T. and Dubcovsky, J. (2003) Positional Cloning of the Wheat Vernalization Gene VRN1. Proceedings of the National Academy of Sciences of the United States of America, 100, 6263-6268. https://doi.org/10.1073/pnas.0937399100
Murai, K., Murai, R. and Ogihara, Y. (1997) Wheat MADS Box Genes, a Multigene Family Dispersed Throughout the Genome. Genes and Genetic Systems, 72, 317-321. https://doi.org/10.1266/ggs.72.317
Murai, K., Murai, R., Takumi, S. and Ogihara, Y. (1998) Cloning and Characterization of cDNAs Corresponding to the Wheat MADS Box Genes. Proceedings of 9th International Wheat Genetic Symposium, Vol. 1, Saskatoon, 2-9 August 1998, 89-94.
Schmitz, J., Franzen, R., Ngyuen, T. H., Garcia-Maroto, F., Pozzi, C., Salamini, F. and Rohde, W. (2000) Cloning, Mapping and Expression Analysis of Barley MADS-Box Genes. Plant Molecular Biology, 42, 899-913. https://doi.org/10.1023/A:1006425619953
Gocal, G.F.W., King, R.W., Blundell, C.A., Schwartz, O.M., Andersen, C.H. and Weigel, D. (2001) Evolution of Floral Meristem Identity Genes. Analysis of Lolium temulentum Genes Related to APETALA1 and LEAFY of Arabidopsis. Plant Physiology, 125, 1788-1801. https://doi.org/10.1104/pp.125.4.1788
Shimada, S., Ogawa, T., Kitagawa, S, Suzuki, T., Ikari, C., Shitsukawa, N., Abe, T., Kawahigashi, H., Kikuchi, R., Handa, H., et al. (2009) A Genetic Network of Flowering Time Genes in Wheat Leaves, in Which an APETALA1/FRUITFULL-Like Gene, VRN1, Is Upstream of Flowering locus T. The Plant Journal, 58, 668-681. https://doi.org/10.1111/j.1365-313X.2009.03806.x
Nishiura, A., Kazama, Y., Abe, T., Mizuno, N., Nasuda, S. and Murai, K. (2014) Level of VERNALIZATION 1 Expression Is Correlated with Earliness in Extra Early-Flowering Mutant Wheat Lines. Breeding Science, 64, 213-221. https://doi.org/10.1270/jsbbs.64.213
Tanaka, C., Itoh, T., Iwasaki, Y., Mizuno, N., Nasuda, S. and Murai K. (2018) Direct Interaction between VRN1 Protein and the Promoter Region of the Wheat FT Gene. Genes and Genetic Systems, 93, 25-29. https://doi.org/10.1266/ggs.17-00041
Theißen, G., Melzer, R. and Rümpler, F. (2016) MADS-Domain Transcription Factors and the Floral Quartet Model of Flower Development: Linking Plant Development and Evolution. Development, 143, 3259-3271. https://doi.org/10.1242/dev.134080
Hugouvieux, V., Silva, C.S., Jourdain, A., Stigliani, A., Charras, Q., Conn, V., Conn, S.J., Carles, C.C., Parcy, F. and Zubieta, C. (2018) Tetramerization of MADS Family Transcription Factors SEPALLATA3 and AGAMOUS Is Required for Floral Meristem Determinacy in Arabidopsis. Nucleic Acids Research, 46, 4966-4977. https://doi.org/10.1093/nar/gky205
Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M.R., Appel, R.D. and Bairoch, A. (2005) Protein Identification and Analysis Tools on the ExPASy Server. In: Walker, J.M., Ed., The Proteomics Protocols Handbook, Humana Press, New York, 571-607. https://doi.org/10.1385/1-59259-890-0:571
Kaufmann, K., Melzer, R. and Theißen, G. (2005) MIKC-Type MADS-Domain Proteins: Structural Modularity, Protein Interactions and Network Evolution in Land Plants. Gene, 347, 183-198. https://doi.org/10.1016/j.gene.2004.12.014
Yang, Y. and Jack, T. (2004) Defining Subdomains of the K Domain Important for Protein-Protein Interactions of Plant MADS Proteins. Plant Molecular Biology, 55, 45-59. https://doi.org/10.1007/s11103-004-0416-7
Deng, W., Casao, M.C., Wang, P., Sato, K., Hayes, P.M., Finnegan, E.J. and Trevaskis, B. (2015) Direct Links between the Vernalization Response and Other Key Traits of Cereal Crops. Nature Communications, 6, Article No. 5882. https://doi.org/10.1038/ncomms6882
Espinosa-Soto, C., Immink, R.G.H., Angenent, G.C., Alvarez-Buylla, E.R. and de Folter, S. (2014) Tetramer Formation in Arabidopsis MADS Domain Proteins: Analysis of a Protein-Protein Interaction Network. BMC Systems Biology, 8, Article No. 9. https://doi.org/10.1186/1752-0509-8-9