Investigation on Viral Pathogens in <i>Vitis vinifera</i> from Four Production Bases in Hangzhou Vicinity of China by sRNAseq and Molecular Validation
- 1 Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hang-zhou, China
- 2 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
- 3 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
- 4 Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
Abstract
This is the first systematic investigation of viral pathogens in Vitis vinifera from Hangzhou vicinity of China. About 7 viruses and 5 viroids were annotated from four production bases “Dushicun”, “Wangjiayuan”, “Xiajiangcun”, and “Yangducun” covering 15 cultivars through sRNAseq technique. At least 3 viruses —grapevine leaf roll-associated virus 3 (GLRaV-3), grapevine fleck virus (GFkV) and grapevine geminivirus A (GGVA), and 4 viroids—hop stunt viroid (HSVd), citrus viroid II (CVd-II), grapevine yellow speckle viroid 1 (GYSVd-1) and grapevine yellow speckle viroid 2 (GYSVd-2) infected all four bases. “Yangducun” base showed 11, the most infected pathogens. GYSVd-1 showed the highest accumulation in host of Wangjiayuan base. The main in fected pathogens were verified by reverse-transcription polymerase chain reaction (RT-PCR) technique, the detected rate reached to 85% - 100%. The results provide an important basis for effective and precise detection of viral diseases in the area and for the virus-free cultivation in future.
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