Aloe vera (L.) Burm.f. is one of the important medicinal plants, has been commercially cultivated in the Northern part of Bangladesh. An experiment was conducted to detect A. vera leaf disease collected from Natore district, Bangladesh. Fungal leaf spot disease caused by Pseudopestalotiopsis theae (Sawada) Maharachch., K.D. Hyde & Crous was identified through morphological features and sequencing of internal transcribed spacer region of ribosomal DNA. After submitting nucleotide sequences to NCBI, we received an accession number MH333081.1: Pseudopestalotiopsis theae . The growth pattern of the isolated fungal pathogen was evaluated on different solid culture media, at different temperature and light conditions. The results showed the maximum mycelial growth of the fungus on the Richard Agar medium under the complete dark condition at 25°C. We evaluated fungal antagonists against the isolated pathogenic fungus, in which Trichoderma asperellum showed optimistic results. Synthetic fungicides —Tilt 250EC and Ridomil Gold completely inhibited the studied fungus’s vegetative growth. Pseudopestalotiopsis theae causing A. vera leaf spot disease in Bangladesh is a new record to the best of our knowledge.
Heggers, J.P., Kucukcelebi, A., Listengarten, D., Stabenau, J., Ko, F., Broemeling, L.D., Robson, M.C. and Winters, W.D. (1996) Beneficial Effect of Aloe on Wound Healing in an Excisional Wound Model. The Journal of Alternative and Complementary Medicine, 2, 271-277. https://doi.org/10.1089/acm.1996.2.271
Ahmmed, S.M., Sikder, M.M., Sultana, A., Sultana, S. and Alam, N. (2020) First Report on Leaf Spot Disease of Aloe vera Caused by Alternaria alternata (Fr.) Keissler in Bangladesh. International Journal of Botany Studies, 5, 164-169. http://www.botanyjournals.com/archives/2020/vol5/issue5/5-5-34
Begum, F., Rahman, M.A. and Alam, M.F. (2010) Biological Control of Alternaria Fruit Rot of Chili by Trichoderma Species under Field Conditions. Mycobiology, 38, 113-117. https://doi.org/10.4489/MYCO.2010.38.2.113
Ahmmed, S.M., Sikder, M.M., Sultana, A., Sultana, S. and Alam, N. (2021) First Report of Leaf Spot Disease of Aloe vera Caused by Fusarium oxyporum in Bangladesh. International Journal of Progressive Sciences and Technologies, 29, 251-260. https://ijpsat.es/index.php/ijpsat/article/view/3574
Azad, R., Hamza, A., Khatun, M.M., Nasrin, S., Tanny, T., Das, K.C., Salimullah, M. and Alam, I. (2020) First Report of Colletotrichum siamense Causing Leaf Spot in Aloe vera in Bangladesh. Plant Disease, 104, 3058. https://doi.org/10.1094/PDIS-04-20-0857-PDN
Chen, Y., Zeng, L., Shu, N., Jiang, M., Wang, H., Huang, Y. and Tong. H. (2018) Pestlotiopsis-Like Species Causing Gray Blight Disease on Camellia sinensis in China. Plant Disease, 102, 98-106. https://doi.org/10.1094/PDIS-05-17-0642-RE
Alam, N., Lee, J. and Lee, T. (2010) Mycelial Growth Conditions and Molecular Phylogenetic Relationships of Pleurotus ostreatus. World Applied Sciences Journal, 9, 928-937. https://www.idosi.org/wasj/wasj9(8)/13.pdf
Liu, F., Hou, L., Raza, M. and Cai, L. (2017) Pestalotiopsis and Allied Genera from Camellia, with Description of 11 New Species from China. Scientific Reports, 7, Article No. 866. https://doi.org/10.1038/s41598-017-00972-5
Aktar, M. and Shamsi, S. (2014). Report on Alternaria Blight of Tagetes erecta and Tagetes patula Caused by Alternaria alternata (Fr.) Keissler. Journal of the Asiatic Society of Bangladesh, Science, 40, 133-140. https://doi.org/10.3329/jasbs.v40i1.31740
White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., Eds., PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., New York, 315-322.
Sikder, M.M., Mallik, M.R.I. and Alam, N. (2019) Identification and in Vitro Growth Characteristics of Entomopathogenic fungus-Aschersonia sp. in Bangladesh. Advances in Zoology and Botany, 7, 11-18. https://doi.org/10.13189/azb.2019.070102
Mallik, M.R.I., Sikder, M.M., Hossain, M.K., Billah, M.B. and Alam, N. (2021) Molecular Characterization and in Vitro Control Measures of Fruit Rot Disease of Sweet Pepper. International Journal of Agricultural Research Innovation and Technology, 11, 108-116. https://doi.org/10.3329/ijarit.v11i2.57263
Sultana, S., Sikder, M.M., Ahmmed, M.S., Sultana, A. and Alam, N. (2020) Mycelial Growth and Biological Control Measures of Botrytis cinerea Isolated from Strawberry Fruit rot Disease in Bangladesh. Universal Journal of Microbiology Research, 8, 13-18. https://doi.org/10.13189/ujmr.2020.080201
Sikder, M.M., Ahmmed, M.S., Sultana, A. and Alam, N. (2020) First Report on Black Spot Disease of Phyllanthus emblica L. Fruits Caused by Thielaviopsis paradoxa in Bangladesh. International Journal of Agricultural Research Innovation and Technology, 10, 38-46. https://doi.org/10.3329/ijarit.v10i2.51575
Rahman, S.M., Sikder, M.M., Nusrat, S. and Khair, A. (2015) In Vitro Evaluation of Botanical Extract, Bioagents and Fungicides against Purple Blotch Diseases of Bunch Onion in Bangladesh. Advances in Zoology and Botany, 3, 179-183. https://doi.org/10.13189/azb.2015.030403
Billah, M.B., Sikder, M.M., Mallik, M.R.I., Hossain, M.K. and Alam, N. (2021) Molecular Characterization and Vegetative Growth of Pathogenic Seed-Borne Fungus Curvularia lunata of Tomato and It’s in Vitro Control Measures. International Journal of Agricultural Research Innovation and Technology, 11, 124-132. https://doi.org/10.3329/ijarit.v11i2.57265
Shamoli, F.A., Khair, A., Bhadra, M., Hossain, M.A. and Sikder, M.M. (2016) Symptomatology of Fungal Competitors on Oyster Mushroom’s Spawn Packets and in Vitro Evaluation Using Phytoextracts and a Fungicide. International Journal of Agricultural Research, Innovation and Technology, 6, 24-30. https://doi.org/10.3329/ijarit.v6i2.31701
El-Gali, Z.I. (2017) Effect of Some Ecological Factors on Growth of Pestalotiopsis spp. Isolated from Mastic Shrubs Leaves. Journal of Advanced Botany and Zoology, 5, 1-5. https://scienceq.org/wp-content/uploads/2018/01/347553163.pdf
Fovo, J.D., Dostaler, D. and Bernier, L. (2017) Influence of Culture Media and Temperature on Growth and Sporulation of Lasiodiplodia theobromae, Pestalotiopsis microspora and Fusarium oxysporum Isolated from Ricinodendron heudelotii in Cameroon. International Journal of Current Microbiology and Applied Science, 6, 3098-3112. https://doi.org/10.20546/ijcmas.2017.606.367
Bajo, J., Santamaría, O. and Diez, J.J. (2008) Cultural Characteristics and Pathogenicity of Pestalotiopsis funerea on Cupressus arizonica. Forest Pathology, 38, 263-274. https://doi.org/10.1111/j.1439-0329.2008.00545.x
Shen, H.F., Zhang, J.X., Lin, B.R., Pu, X.M., Zheng, L., Qin, X.D., Li, J. and Xie, C.P. (2014) First Report of Pestalotiopsis microspora Causing Leaf Spot of Oil Palm (Elaeis guineensis) in China. Plant Disease, 98, 1429. https://doi.org/10.1094/PDIS-02-14-0163-PDN
Upadhyay, R.K. and Dwivedi, R.S. (1980) Cultural and Taxonomical Studies on Pestalotiopsis funerea Causing Leaf Spot of Eucalyptus globulus. Proceedings of the Indian National Science Academy, Part B, 46, 397-404.
Keith, L.M., Velasquez, M.E. and Zee, F.T. (2006) Identification and Characterization of Pestalotiopsis spp. Causing Scab Disease of Guava, Psidium guajava, in Hawaii. Plant Disease, 90, 16-23. https://doi.org/10.1094/PD-90-0016
Sapna, M., Peeyush, K. and Anushree, M. (2013) Effect of Process Parameters on the Enzyme Activity of a Novel Beauveria bassiana Isolate. International Journal of Current Microbiology and Applied Sciences, 2, 49-56. https://www.ijcmas.com/vol-2-9/Sapna%20Mishra,%20et%20al.pdf
Pathak, S.S., Kumar, S., Rajak, R.C. and Sandhu, S.S. (2014) Study of Effect of Temperature on Amylase Production by Soil Mycotic Flora of Jabalpur Region. World Journal of Pharmacy and Pharmaceutical Sciences, 3, 1448-1458. https://www.wjpps.com/Wjpps_controller/abstract_id/2045
Bhadra M., Khair, A., Hossain, M.A. and Sikder, M.M. (2012) Biocontrol Potentiality of Trichoderma spp. on Colletotrichum capsici Causing Anthracnose of chilli. Jahangirnagar University Journal of Biological Sciences, 1, 107-115.
Bhadra M., Khair, A., Hossain, M.A., and Sikder, M.M. (2014) Efficacy of Trichoderma spp. and Fungicides on Lasiodiplodia theobromae. Bangladesh Journal of Scientific and Industrial Research, 49, 125-130. https://doi.org/10.3329/bjsir.v49i2.22008
Chowdhury, P., Shamsi, S. and Bashar, M.A. (2015) Grain Spotting of Rice Caused by Pestalotiopsis guepinii (Desm.) Stay—A New Record. Dhaka University Journal of Biological Sciences, 24, 103-106. https://doi.org/10.3329/dujbs.v24i1.46315
Shamsi, S., Chowdhury, P. and Sultana, T. (2014) In Vitro Control of Causal Agents of Anthracnose of Seen aalata L.—A herbal Medicinal Plant in Bangladesh. Medicinal and Aromatic Plant Research Journal, 2, 37-43. http://sciencewebpublishing.net/maprj/archive/2014/3/Abstract/Shamsi%20et%20al.htm