Interaction Effect of Auxin and Cytokinin on <i>in Vitro</i> Shoot Regeneration and Rooting of Endangered Medicinal Plant <i>Valeriana jatamansi</i> Jones through Tissue Culture

Abstract

Micropropagation of Valeriana jatamansi Jones by using small segments of rhizome on full strength MS medium having various concentrations and combinations of auxin Naphthaleneacetic acid (NAA) and cytokinin Benzylaminopurine (BAP) was conducted. The highest mean shoot length (3.71 cm) was achieved when media was fortified with BAP 2 mg/L in combination with NAA 1 mg/L. The highest mean leaf number i.e. 6.00 was observed when BAP was used alone at 2 mg/L. Average root length (0.77 cm) was recorded when BAP 1.5 mg/L along with NAA 0.5 mg/L was used. Maximum mean root numbers 2.57 were obtained when BAP and NAA were used at equal concentrations i.e . 1.5 mg/L. Observed data demonstrated that BAP up to 1 mg/L, 1.5 mg/L and 2 mg/L promotes shoot length, leaf number and leaf growth when used along with NAA at 0 mg/L, 0.5 mg/L and 1 mg/L. However lower quantities of both NAA (0 mg/L, 0.5 mg/L) and BAP (1 mg/L and 1.5 mg/L) produced significantly higher root length of Valeriana jatamansi Jones but the higher concentrations of plant growth hormones BAP (2 mg/L, 3 mg/L) and NAA (1 mg/L, 1.5 mg/L) were found unfavorable for increase in root length but the root number increases at higher concentration of NAA (1 mg/L and 1.5 mg/L).