The present work has been conducted to study the impact of Zn (20 and 200 μM) or Ni (1 and 100 μM) on growth, metabolic activities, osmotic pressure and heavy metal of wheat cultivar (cv. Gimiza 11) under salt stress conditions. Harvest index was significantly decreased by 75% at -0.3 MPa and -1.2 MPa decreased by 53%. Length of root, stem and spike, and leaf area were slightly reduced up to -0.9 MPa NaCl level, after that a significant reduction was recorded. Total sugar was increased in the shoot and spike, while decreased in the root with increasing salinity levels. On the other side, total protein was increased in the shoot while decreased in both the root and spike. Osmotic pressure was increased as increasing salinity levels. Treatment of wheat plants with Zn or Ni alleviated generally salt injury in the harvest index, length of root, shoot and spike and leaf area. This effect was more obvious with Zn treatments. Mineralization treatments increased total sugar in the three tested organs while did not change the total protein. Zn content was increased at -1.2 MPa level while Ni was decreased. Treatments with Zn or Ni mostly increased Zn while causing a reduction in the Ni content. These treatments also increased the values of osmotic pressure compared with the equivalent levels of control plants.
KeywordsOsmotic StressWheatZnNiTolerantSensitive
Shewry, P.R. and Hey, S.J. (2015) Review: The Contribution of Wheat to Human Diet and Health. Food and Energy Security, 4, 178-202. https://doi.org/10.1002/fes3.64 https://onlinelibrary.wiley.com/
Saleh, B. (2012) Effect of Salt Stress on Growth and Chlorophyll Content of Some Cultivated Cotton Varieties Grown in Syria. Communications in Soil Science and Plant Analysis, 43, 1976-1983. https://doi.org/10.1080/00103624.2012.693229 https://www.tandfonline.com/doi/full/10.1080/00103624.2012.693229
Abdelaal, K.A.A., Hafez, M.H., Afry, M.M., Tantawy, D.S. and Alshaal, T. (2018) Effect of Some Osmo-regulators on Photosynthesis, Lipid Peroxidation, Antioxidative Capacity, and Productivity of Barley (Hordeum vulgare L.) under Water Deficit Stress, Environmental Science and Pollution Research, 25, 30199-30211. https://doi.org/10.1007/s11356-018-3023-x https://pubmed.ncbi.nlm.nih.gov/30155630/
Abd El-Samad, H.M., Mostafa, D. and Abd El-Hakeem, K.N. (2017) The Combined Action Strategy of Two Stresses, Salinity and Cu++ on Growth, Metabolites and Protein Pattern of Wheat Plant. American Journal of Plant Sciences, 8, Article ID: 625-643. https://doi.org/10.4236/ajps.2017.83043 https://file.scirp.org/Html/22-2602920_74486.htm
Abd El-Samad, M.H, Hassanien, A.M., Mostafa, D.M. and Taha, R.M. (2019) The Synergistic Effect of Zinc and Nickel with Osmotic Stress on Growth of Four Wheat Cultivars. Merit Research journal of Agricultural Science and Soil Sciences, 5, 187-200. https://www.researchgate.net/publication/352983365_Original_Research_Article_ The_Synergistic_Effect_of_Zinc_and_Nickel_with_Osmotic_Stress_on_Growth_of_Four_Wheat_Cultivars
Jan, R,; Khan, M.A., Asaf, S., Lubna, Lee, I.J. and Kim, K.M. (2019) Metal Resistant Endophytic Bacteria Reduces Cadmium, Nickel Toxicity and Enhances Expression of Metal Stress Related Gene with Improved Growth of Oryza sativa via Regulating Its Antioxidant Machinery and Endogenous Hormones. Plants, 8, Article No. 363. https://doi.org/10.3390/plants8100363 https://pubmed.ncbi.nlm.nih.gov/31547575/
Saleh, J. and And Maftoun, M. (2008) Interactive Effects of NaCl Levels and Zinc Sources and Levels on the Growth and Mineral Composition of Rice. Journal of Agricultural Science and Technology, 10, 325-336. https://jast.modares.ac.ir/article-23-2346-en.html
Ain, Q., Akhtar, J., Amjad, M., Haq, M.A. and Saqib, Z.A. (2016) Effect of Enhanced Nickel Levels on Wheat Plant Growth and Physiology under Salt Stress. Communications in Soil Science Plant Analysis, 47, 2538-2546. https://doi.org/10.1080/00103624.2016.1254796 https://www.researchgate.net/publication/310435107
Jagetiya, B., Soni, A. and Yada, S. (2013) Effect of Nickel on Plant Water Relations and Growth in Green Gram. Indian Journal of Plant Physiology, 18, 372-376. https://doi.org/10.1007/s40502-013-0053-8 https://www.scirp.org/(S(vtj3fa45qm1ean45vvffcz55))/reference/ReferencesPapers.aspx?ReferenceID=1596276
Kumar, O., Singh, S.K., Latare, A.M. and Yadav, S.N. (2018) Effect of Foliar Fertilization of Nickel Affects Growth, Yield Component and Micronutrient Status of Barley (Hordeum vulgare) Grown in Low Nickel Soil. Archives of Agronomy and soil, 64, 1407-1418. https://doi.org/10.1080/03650340.2018.1438600 https://www.tandfonline.com/doi/full/10.1080/03650340.2018.1438600
Kumar, O., Singh, S.K., Singh, A.P., Yadov, S.N. and Latare, A. (2018) Effect of Soil Application of Nickel on Growth, Micronutrient Concentration and Uptake in Barley (Hordeum vulgare L.) Grown in Inceptisols of Varanasi. Plant Nutrition, 41, 50-66. https://doi.org/10.1080/01904167.2017.1381724 https://www.researchgate.net/publication/319994620
Beadle, C.L. (1993) Growth Analysis. In: Hall, D.O., Scurlock, J.M.O., Bolharnordenkampfh, R., Leegood, R.C. and Long, S.P., Eds., Photosynthesis and Production in a Changing Environment: A Field and Laboratory Manual, Chapman and Hall, London, 36-46. https://www.scirp.org/(S(i43dyn45teexjx455qlt3d2q))/reference/ReferencesPapers.aspx?ReferenceID=1987112
McKee, G.W. (1974) A Coefficient for Computing Leaf Area in Hybrid Corn. Agronomy Journal, 56, 240-241. https://doi.org/10.2134/agronj1964.00021962005600020038x
Bonhomme, R., Varlet, M., Grancher C. and Chartier P. (1974) The Use of Hemispherical Photographs for Determining Leaf Index of Young Crops. Photosynthetica, 8, 299-301.
Norman, J. and Campbell G.S. (1994) Canopy Structure. In: Pearcy, R.W., Ehleringer, J.R., Moony, H.A. and Rundel, P.W., Eds., Plant Physiological Ecology, Chapman & Hall, London, 301-325. https://doi.org/10.1007/978-94-010-9013-1_14
Fales, F.W. (1951) The Assimilation and Degradation of Carbohydrate by Yeast Cells. Journal of Biology and Chemistry, 193, 113-124. https://doi.org/10.1016/S0021-9258(19)52433-4 http://www.jbc.org/content/193/1/113.full.pdf
Lowry, O.H., Rasebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein Measurement with the Folin Phenol Reagent. Journal of Biology and Chemistry, 193, 265-275. https://doi.org/10.1016/S0021-9258(19)52451-6 https://en.wikipedia.org/wiki/Journal_of_Biological_Chemistry
Steel, R.G. and Torrie, J.H. (1960) Principles and Procedures of Statistics. McGraw-Hill Book Co., New York. http://garfield.library.upenn.edu/classics1977/A1977DU23500002.pdf
Kamran, M., Parveen, A., Ahmar, S., Malik, Z., Hussain, S., Chattha, M.S., Saleem, M.H., Adil, M., Heidari, P. and Chen, J.T. (2019) An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation. International Journal of Molecular Sciences, 21, Article No. 148. https://doi.org/10.3390/ijms21010148 https://www.mdpi.com/1422-0067/21/1/148
Shahid, M.A., Sarkhosh, A., Khan, N, Balal, R.M., Ali, S., Rossi, L., Gomez, C., Mattson, N., Nasim, W. and Garacia-Sanchez, F. (2020) Insights into the Physiological and Biochemical Impacts of Salt Stress on Plant Growth and Development. Agronomy, 10, Article No. 938. https://doi.org/10.3390/agronomy10070938 https://www.mdpi.com
Rajput, V.D., Minkina,T., Kumari, A., Harish, Singh,V.K., Sushkova, S., Srivastava, S., Verma, K.K., Mandzhieva, S., Sudhakar, S., Srivastava, S. and Keswani, C. (2021) Coping with the Challenges of Abiotic Stress in Plants: New Dimensions in the Field Application of Nanoparticles. Plants, 10, Article No. 1221. https://doi.org/10.3390/plants10061221
Karimi, H.R. and Kuhbanani, R. (2014) Evaluation of Inter-specific Hyprid of Pistachio altantica X Pistachio vera cv. Badami-Riz-zarand as Pistachio Root Stock to Salinity Stress According to Some Growth Indices, Eco-physiological and Biochemical Parameters. Journal of Stress Physiology and Biochemistry, 10, 5-17. https://www.researchgate.net/publication/265382108
Rahneshan, Z., Nasibi, F. and Moghadam, A.A. (2018) Effects of Salinity on Some Growth, Physiological, Biochemical Parameters and Nutrients in Two Pistachio (Pistacia vera L.) Root Stocks. Journal of Plant Interactions, 13, 73-82. https://doi.org/10.1080/17429145.2018.1424355 https://www.tandfonline.com/doi/full/10.1080/17429145.2018.1424355
Rajput, V.D., Yaning, C., Ayup, M., Minkina, T., Sushkova, S. and Mandzhieva, S. (2017) Physiological and Hydrological Changes in Populus euphratica Seedlings under Salinity Stress. Acta Ecologica Sinica, 37, 229-235. https://doi.org/10.1016/j.chnaes.2017.02.005
Rani, S., Sharma, M.K., Kumar, N. and Neelam (2019) Impact of Salinity and Zinc Application on Growth, Physiological and Yield Traits in Wheat. Current Science, 8, 1324-1330. https://doi.org/10.18520/cs/v116/i8/1324-1330 https://www.researchgate.net/publication/334049940
Arbona, V., Marco, J., Iglesias, D.J., López-Climent, M.F., Talon M. and Gómez-Cadenas, A. (2005) Carbohydrate Depletion in Roots and Leaves of Salt-stressed Potted Citrus clementina L. Plant Growth Regulation, 46,153-160. https://doi.org/10.1007/s10725-005-7769-z
Moradi, F. and Ismail, A.M. (2007) Responses of Photosynthesis, Chlorophyll Fluorescence and ROS Scavenging Systems to Salt Stress During Seedling and Reproductive Stages in Rice. Annual Botany Journal, 99, 1161-1173. https://doi.org/10.1093/aob/mcm052 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243573/
Benabderrahim, M.A., Guiz, A.M. and Haddad, M. (2020) Genentic Diversity of Salt Tolerane in Tetraploid Alfalfa (Medicago sativa L.). Acta Phsiologiae Plantarum, 24, Article No. 5. https://doi.org/10.1007/s11738-019-2993-8 https://www.semanticscholar.org/paper/
Koselski, M., Trebacz, K. and Dziibinska, H. (2019) The Role of Vacuolar Ion Channels in Salt Stress Tolerance in the Liverwort Chenopodium coricum. Acta Physiologiae Plantarum, 41, Article No. 110. https://doi.org/10.1007/s11738-019-2889-7
Abd El-Samad, M.H, Hassanien, A.M., Mostafa, D.M. and Taha, R.M. (2019) The Physiological Strategy of Zinc and Nickel in Improvement of Osmotic Defense System of Two Wheat Cultivars. Sylwan, 64, 1-23.
Bagheri, A. and Sadeghipour, O. (2009) Effects of Salt Stress on Yield, Components and Carbohydrates Content on Four Hull Less Barely (Hordeum Vulgar L.) Cultivars. Journal of Biology Science, 9, 909-912. https://doi.org/10.3923/jbs.2009.909.912 https://scialert.net/abstract/?doi=jbs.2009.909.912
Abd El-Samad, H.M.H.A. (2016) The Potential Role of Osmotic Pressure to Exogenous Application of Phytohormones on Crop Plants Grown under Different Osmotic Stress. American Journal of Plant Sciences, 7, 937-948. https://doi.org/10.4236/ajps.2016.76089 http://www.scirp.org/journal/PaperInformation.aspx?paperID=65969
Torre-Gonzalez, A.D., Leon, E.N., Blasco, B. and Ruiz, J.M. (2020) Nitrogen and Photorespiration Pathway, Salt Stress Genotypic Tolerance Effects in Tomato Plants (Solanum lyopersicum L.). Acta Physioloiae Plantarum, 42, Article No. 2. https://doi.org/10.1007/s11738-019-2985-8
Ashraf, M. and Harris, P.J.C. (2004) Potential Biochemical Indicators of Salinity Tolerance in Plant. Plant Science, 166, 3-16. https://doi.org/10.1016/j.plantsci.2003.10.024 https://www.sciencedirect.com/science/article/pii/S0168945203004679
Jiang, X., Qi, W., Xu, X., Li, Y., Liao, Y. and Wang, B. (2014) Higher Soil Salinity Causes More Physiological Stress in Female of Populous cathayana Cuttings. Acta Ecologica Sinica, 34, 225-231. https://doi.org/10.1016/j.chnaes.2014.05.002 https://agris.fao.org/agris-search/search.do?recordID=US201700150693
Manivasagaperumal, R., Vijayaranjan, P., Balamurugan, S., and Thiyagarajan, G. (2011) Effect of Copper on Growth, Dry Matter Yield and Nutrient Content of Vigna radiata (L.) Wilczek. Journal of Phytology, 3, 52-62. https://updatepblishing.com>view
Osakabe, Y., Osakabe K., and Tran L.P. (2014) Response of Plants to Water Stress. Frontiers in Plant Science, 5, Article No. 86. https://doi.org/10.3389/fpls.2014.00086 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3952189
Alves, ZA.A.G. and Setter, T.L. (2004) Abscisic Acid Accumulation and Osmotic Adjustment in Cassava under Water Deficit. Environmental and Experimental Botany, 51, 259-279. https://doi.org/10.1016/j.envexpbot.2003.11.005 https://www.infona.pl/resource/bwmeta1.element.elsevier-3bb926c8-a890-3493-a735-b135d10a490f
Abd El-Samad, H.M., Shaddad, M.A.K. and Ragaey, M.M. (2019) Drought Strategy Tolerance of Four Barley Cultivars and Combined Effect with Salicylic Acid Application. American Journal of Plant Sciences, 10, 512-535. https://doi.org/10.4236/ajps.2019.104037 https://www.scirp.org/journal/paperinformation.aspx?paperid=91837
Mohammed, H.A.M. (2007) Physiological Studies on the Antioxidative Responses and Some Related Metabolities of Lupin and Sorghum Plants Grown under Sea Water M.Sc. Thesis, South Valley University, Qena, 1-184. https://www.svu.edu.eg/en
Khan, H., Mcdonald, G. and Rengel, Z. (2004) Zin Fertilization and Water Stress Affects Plant Water Relations, Stomata Conductance and Osmotic Adjustment in Chickpea (Cicer arientinum L.). Plant and Soil, 267, 271-284. https://doi.org/10.1007/s11104-005-0120-7
Bhatia, N.P., Baker, A.J., M., Walsh, K.B. and Midmore, D.J. (2005) A Role Nickel in Osmotic Adjustment in Drought-stressed Plants of the Nikel Hyperaccumulator Stackhousia Tryonii Bailey. Plana, 223, 134-139. https://doi.org/10.1007/s00425-005-0133-8
Hussein, M.M. and Abou-Baker, N.H. (2018) The Contribution of Nano-zinc to Alleviate Salinity Stress on Cotton Plants. Royal Society Open Science, 5, Article ID: 171809. https://doi.org/10.1098/rsos.171809 https://royalsocietypublishing.org
Tavallali, V. (2016) The Effectiveness of Zinc in Alleviating Salinity Stress on Pistachio Seedlings. Fruits, 71, 433-445. https://doi.org/10.1051/fruits/2016034 https://fruits.edpsciences.org/articles/fruits/pdf/2016/06/fruits150190.pdf
Iqbal, M.N., Rasheed, R., Hussain, I., Muhammad, Y.A. and Muhammad, A.A. (2018) Exogenously Applied Zinc and Copper Mitigate Salinity Effect in Maize (Zea mays L.) by Improving Key Physiological and Biochemical Attributes. Environmental Science and Pollution Research, 25, 23883-23896. https://doi.org/10.1007/s11356-018-2383-6 https://pubmed.ncbi.nlm.nih.gov/29881963/
Stetsenko, L.A., Kozhevnikova, A.D. and Kartashov, A.V. (2017) Salinity Attenuates Nickel-accumulating Capacity of Atropa belladonna L. Plants. Russian Journal of Plant Physiology, 64, 486-496. https://doi.org/10.1134/S102144371704015X https://link.springer.com/article/10.1134/S102144371704015X
Sharma, A. and Dhiman, A. (2013) Nickel and Cadmium Toxicity in Plants. Journal of Pharmaceutical and Scientific Innovation, 2, 20-24. https://doi.org/10.7897/2277-4572.02213 https://www.researchgate.net/publication/272770423
Nie, J., Pan, Y.; Shi, J., Guo Yan, Z., Duan, X. and Xu, M. (2015) A Comparative Study on the Uptake and Toxicity of Nickel Added in the Form of Different Salts to Maize Seedlings. International Journal of Environmental Research, Public Health, 12, 15075-15087. https://doi.org/10.3390/ijerph121214972 https://pubmed.ncbi.nlm.nih.gov/26633435/
Mohamed, A.M., Abdeldaym, E.A., Abdelaziz, S.M., Sawy, M.B.I. and Mottaleb, S.A. (2020) Synergistic Effect of Zinc, Boron, Silicon, and Zeolite Nanoparticles on Tolerance of Potato Plants Subjected to Salinity. Agronomy, 10, Article No. 19. https://doi.org/10.3390/agronomy10010019 https://www.mdpi.com/2073-4395/10/1/19