Fungitoxicity of <i>Trichoderma longibrachiatum</i> (Rifai) Metabolites against <i>Fusarium oxysporum</i>, <i>Aspergillus niger</i> and <i>Aspergillus tamarii</i> — Oak Academic Publishing
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Fungitoxicity of <i>Trichoderma longibrachiatum</i> (Rifai) Metabolites against <i>Fusarium oxysporum</i>, <i>Aspergillus niger</i> and <i>Aspergillus tamarii</i>
Department of Botany, University of Ibadan, Ibadan, Nigeria
,
Nigerian Institute of Science Laboratory Technology (NISLT), Ibadan, Nigeria
,
Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria
,
Department of Biological Sciences, Lead City University, Ibadan, Nigeria
1 Department of Botany, University of Ibadan, Ibadan, Nigeria
2 Nigerian Institute of Science Laboratory Technology (NISLT), Ibadan, Nigeria
3 Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria
4 Department of Biological Sciences, Lead City University, Ibadan, Nigeria
An experiment was carried out to evaluate the fungitoxic effect of Trichoderma longibrachiatum (Rifai) metabolite on F. oxysporum , A. niger and A. tamarii . The fungi were collected from the International Institute of Tropical Agricult ure (IITA) and Nigerian Institute of Science Laboratory Technology (NISL T ). T. longibr achiatum was cultured on 1/4 strength Potato Dextrose Broth (PDB) following standard procedures. Its metabolite was extracted using 50 ml n-hexane with 50 ml Potato Dextrose Broth (PDB). The metabolite was purifi ed by filter steril ization using a sterile 0.22 millipore filter disc after centrifuging at 900 rpm for 20 m inutes. Petri plates of each fungus were later impregnated in triplicates with the T. longibrachiatum metabolites using four concentrations (10%, 25%, 50% and 100%), and three volumes (1 ml, 2 ml, and 3 ml). Petr i plates of fungi without the metabolite and Petri Plates of fungi with n-hexane and PDB served as control. All Petri Plates were incubated at 28 °C for 7 days. Radial and diametric growth of each fungus on all Petri Plates w ere taken daily at 24 hours interval s . Data obtained were analysed using SAS (version 9.3). Growth inhibition of F. oxysporum , A. niger and A. tamarii was significantly higher than in control in that order (P ≤ 0.05). Inhibition of the fungi by metabolites extracted with both PDB and n-hexane was significantly better than in control. Generally, inhibition by metabolite extracted with PDB was better than that extracted with n-hexane. Growth inhibition at all the concentrations of the metabolite was significantly better than in the control (P ≤ 0.05). Generally, inhibition at 100%, 50% and 25% concentrations was better than that at 10% concentration of the metabolite. Trichoderma longibrachiatum metabolite can thus be said to possess promising fungitoxic potenti al even at concentration s as low as 25%. Both PDB and n-hexane are good e xtraction media for the metabolite.
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