Differential Effects of Cold and Heat Shock on Embryogenic Induction and Green Plant Regeneration from Wheat (<i>Triticum aestivum</i> L.) Microspores — Oak Academic Publishing
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Differential Effects of Cold and Heat Shock on Embryogenic Induction and Green Plant Regeneration from Wheat (<i>Triticum aestivum</i> L.) Microspores
Department of Biology, Gordon College, Wenham, MA, USA
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Department of Biology, Gordon College, Wenham, MA, USA
,
Cell Signaling Technology, Inc., Danvers, MA, USA
1 Department of Biology, Gordon College, Wenham, MA, USA
2 Department of Biology, Gordon College, Wenham, MA, USA
3 Cell Signaling Technology, Inc., Danvers, MA, USA
Albinism is a common problem encountered by researchers in anther/microspore cultures of cereal crops. The present study investigates the effects of temperature variations on embryogenesis of wheat ( Triticum aestivum L.) microspores. Following a cold (4 °C - 13°C) vs. heat (33°C) shock to wheat tillers, microspores were isolated and cultured in a liquid medium to obtain embryoids. Data on embryogenic microspore%, embryoid yield, plant regeneration% and green plant% were collected and analyzed. Cold pretreatment of 4°C or 10°C for a period of 6 or 10 days were more effective than other cold temperature regimes in inducing microspore embryogenesis. The heat shock of 33°C yielded the highest numbers of embryogenic microspores and embryoids. The albino-prone genotypes produced significantly higher green plant% following optimal cold shock, as compared to the standard 33°C heat shock. Results from present study suggest that cold shock may be a desirable alternative for germplasm that produce lower green plant% using heat shock. Lowered incubation temperature during embryoid development did not result in higher green plant.
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