Rhizobia, crucial for nitrogen fixation in leguminous plants, play a vital role in soybean cultivation. This study, conducted in Mexico, a major soybean importer, aimed to identify bacteria from nodules of five soybean varieties in high-production regions. Multilocus sequence analysis (MLSA) was employe d for enhanced species resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA 76, Bradyrhizobium neotropicale , Bradyrhizobium lablabi , and Bradyrhizobium icense . Bradyrhizobium japonicum USDA 110 predominated in the soils, displaying symbiotic preference for the Huasteca 400 variety. However, phylogenetic analysis didn't reveal a clear association between strains, soil, and soybean variety. This research sheds light on the diversity of rhizobia in Mexican soybean cultivation, contributing to the understanding of symbiotic relationships in soybean production systems.
Food and Agriculture Organization of the United Nations (2023) Land & Water. https://www.fao.org/land-water/databases-and-software/crop-information/soybean/en/
Maldonado-Moreno, N., Ascencio-Luciano, G., Espinosa-Vásquez, G. and de los ángeles Peña del Río, M. (2013) Estrategias Tecnológicas Para Contrarrestar La Sequía En La Producción de Soya En El Sur de Tamaulipas. Villa Cuauhtémoc, Tam.
Servicio de Información Agroalimentaria y Pesquera. (2023) Resumen Por Estado. Obtenido de Avance de Siembras y Cosechas 1-1. http://infosiap.siap.gob.mx:8080/agricola_siap_gobmx/ResumenProducto.do
Díaz-Franco, A., Alejandro-Allende, F., Cisneros-López, Ma.E., Espinosa-Ramírez, M. and Ortíz-Cháirez, F.E. (2021) Biological, Organic and Reduced Mineral Fertilization in Soybean (Glycine Max L.). Terra Latinoamericana, 39, 1-9. e725. https://doi.org/10.28940/terra.v39i0.725
Gitonga, N.M., Njeru, E.M., Cheruiyot, R. and Maingi, J.M. (2021) Bradyrhizobium Inoculation Has a Greater Effect on Soybean Growth, Production and Yield Quality in Organic than Conventional Farming Systems. Cogent Food & Agriculture, 7, Article ID: 1935529. https://doi.org/10.1080/23311932.2021.1935529
Mirriam, A., Mugwe, J., Nasar, J., Kisaka, O., Ranjan, S. and Gitari, H. (2023) Role of Phosphorus and Inoculation with Bradyrhizobium in Enhancing Soybean Production. Advances in Agriculture, 2023, Article ID: 3231623. https://doi.org/10.1155/2023/3231623
Delamuta, J.R.M,, Ribeiro, R.A., Menna, P., Villamil Bangel, E. and Hungria, M. (2012) Multilocus Sequence Analysis (MLSA) of Bradyrhizobium Strains: Revealing High Diversity of Tropical Diazotrophic Symbiotic Bacteria. Brazilian Journal of Microbiology, 43, 698-710. https://doi.org/10.1590/S1517-83822012000200035
Li, Y.H., Wang, R., Hua Sui, X., Wang, E.N., Zhang, X.X., Tian, C.F., Chen, W.F. and Chen, W.X. (2019) Bradyrhizobium Nanningense Sp. Nov., Bradyrhizobium Guangzhouense Sp. Nov. and Bradyrhizobium Zhanjiangense Sp. Nov., Isolated from Effective Nodules of Peanut in Southeast China. Systematic and Applied Microbiology, 42, Article ID: 126002. https://doi.org/10.1016/j.syapm.2019.126002
Hassen, A.I., Bopape, F.L., van Vuuren, A., hegro Gerrano, A. and Morey, L. (2023) Symbiotic Interaction of Bambara Groundnut (Vigna Subterranea) Landraces with Rhizobia Spp. from Other Legume Hosts Reveals Promiscuous Nodulation. South African Journal of Botany, 160, 493-503. https://doi.org/10.1016/j.sajb.2023.07.032
Zhang, J., Wang, N., Li, S., Brunel, B., Wang, J., Feng, Y., Yang, T. and Zong, X. (2023) Genotypic Composition and Performance of Pea-Nodulating Rhizobia from Soils Outside the Native Plant-Host Range. Frontiers in Microbiology, 14, Article 1201140. https://doi.org/10.3389/fmicb.2023.1201140
Kruschewsky, R., Ferreira, M., Cordeiro Silva, V., Ferreira dos Santos, J.M., édson Zilli, J., James, E.K., Fragomeni Simon, M. and Gross, E. (2021) The Large Mimosoid Genus Inga Mill. (Tribe Ingeae, Caesalpinioideae) Is Nodulated by Diverse Bradyrhizobium Strains in Its Main Centers of Diversity in Brazil. Systematic and Applied Microbiology, 44, 126-268. https://doi.org/10.1016/j.syapm.2021.126268
Ferraz H., Caroline, L., O’Hara, G. and Hungria, M. (2020) Characterization of Bradyrhizobium Strains Indigenous to Western Australia and South Africa Indicates Remarkable Genetic Diversity and Reveals Putative New Species. Systematic and Applied Microbiology, 43, Article ID: 126053. https://doi.org/10.1016/j.syapm.2020.126053
Ferraz H., Caroline, L., Serenato Klepa, M. and Hungria, M. (2022) New Insights into the Taxonomy of Bacteria in the Genomic Era and a Case Study with Rhizobia. International Journal of Microbiology, 2022, Article ID: 4623713. https://doi.org/10.1155/2022/4623713
Rivera-Orduña, F.N., Pineda-Mendoza, R.M., Vega-Correa, B., López, M.F., Cano-Ramírez, C., Zhang, X.X., Chen, W.F. and Zúñiga, G. (2023) A Polyphasic Taxonomy Analysis Reveals the Presence of an Ecotype of Rahnella Contaminans Associated with the Gut of Dendroctonus-Bark Beetles. Frontiers in Microbiology, 14, Article 1171164. https://doi.org/10.3389/fmicb.2023.1171164
Liu, H., Cui, Y., Zhou, J., Penttinen, P., Liu, J., Zeng, L., Chen, Q., Gu, Y., Zou, L., Zhao, K., Xiang, Q. and Yu, X. (2022) Nickel Mine Soil Is a Potential Source for Soybean Plant Growth Promoting and Heavy Metal Tolerant Rhizobia. PeerJ, 10, e13215. https://doi.org/10.7717/peerj.13215
Azevedo, H., Martins Lopes, F., Silla, P.R. and Hungria, M. (2015) A Database for the Taxonomic and Phylogenetic Identification of the Genus Bradyrhizobium Using Multilocus Sequence Analysis. BMC Genomics, 16, Article No. S10. https://doi.org/10.1186/1471-2164-16-S5-S10
Diario Oficial de la Federación (2002) NOM-021-RECNAT-2000: Especificaciones de Fertilidad, Salinidad y Clasificación de Suelos. Mexico.
Somasegaran, P. and Hoben, H.J. (1985) Methods in Legume-Rhizobium Technology. University of Hawaii Press, Maui.
Mwangi, S.N., Karanja, N.K., Boga, H., Kahindi, J.H.P., Muigai, A., Odee, D. and Mwenda, G.M. (2011) Genetic Diversity and Symbiotic Efficiency of Legume Nodulating Bacteria from Different Land Use Systems in Taita, Kenya. Tropical and Subtropical Agroecosystems, 13, 109-118.
CIAT (Centro Internacional de Agricultura Tropical) (1988) Simbiosis Leguminosa-Rizobio; Manual de Métodos de Evaluación, Selección y Manejo Agronómico.
Stȩpkowski, T., Moulin, L., Krzyzańska, A., McInnes, A., Law, I.J. and Howieson, J. (2005) European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa. Applied and Environmental Microbiology, 71, 7041-7052. https://doi.org/10.1128/AEM.71.11.7041-7052.2005
Martens, M., Dawyndt, P., Coopman, R., Gillis, M., De Vos, P. and Willems, A. (2008) Advantages of Multilocus Sequence Analysis for Taxonomic Studies: A Case Study Using 10 Housekeeping Genes in the Genus Ensifer (Including Former Sinorhizobium). International Journal of Systematic and Evolutionary Microbiology, 58, 200-214. https://doi.org/10.1099/ijs.0.65392-0
Stepkowski, T., Swiderska, A., Miedzinska, K., Czaplinska, M., Swiderski, M., Biesiadka, J. and Legocki, A.B. (2003) Low Sequence Similarity and Gene Content of Symbiotic Clusters of Bradyrhizobium Sp. WM9 (Lupinus) Indicate Early Divergence of ‘“Lupin” Lineage in the Genus Bradyrhizobium. Antonie van Leeuwenhoek, 84, 115-124. https://doi.org/10.1023/A:1025480418721
Stepkowski, T., Czaplínska, M., Miedzinska, K. and Moulin, L. (2003) The Variable Part of the DnaK Gene as an Alternative Marker for Phylogenetic Studies of Rhizobia and Related Alpha Proteobacteria. Systematic and Applied Microbiology, 26, 483-494. https://doi.org/10.1078/072320203770865765
Edgar, R.C. (2010) Search and Clustering Orders of Magnitude Faster than BLAST. Bioinformatics, 26, 2460-2461. https://doi.org/10.1093/bioinformatics/btq461
Kimura, M. (1980) A Simple Method for Estimating Evolutionary Rates of Base Substitutions through Comparative Studies of Nucleotide Sequences. Journal of Molecular Evolution, 16, 111-120. https://doi.org/10.1007/BF01731581
Kumar, S., Stecher, G. and Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870-1874. https://doi.org/10.1093/molbev/msw054
Rumpel, C. and Kögel-Knabner, I. (2011) Deep Soil Organic Matter—A Key But Poorly Understood Component of Terrestrial C Cycle. Plant and Soil, 338, 143-158. https://doi.org/10.1007/s11104-010-0391-5
Sun, Q., Zhang, P., Zhao, Z., Li, X., Sun, X. and Jiang, W. (2023) Continuous Wheat/Soybean Cropping Influences Soybean Yield and Rhizosphere Microbial Community Structure and Function. Agronomy, 13, Article 28. https://doi.org/10.3390/agronomy13010028
McDaniel, M.D., Tiemann, L.K. and Grandy, A.S. (2014) Does Agricultural Crop Diversity Enhance Soil Microbial Biomass and Organic Matter Dynamics? A Meta-Analysis. Ecological Applications, 24, 560-570. https://doi.org/10.1890/13-0616.1
Li, Y., Shi, C., Wei, D., Gu, X., Wang, Y., Sun, L., Cai, S., Hu, Y., Jin, L. and Wang, W. (2022) Soybean Continuous Cropping Affects Yield by Changing Soil Chemical Properties and Microbial Community Richness. Frontiers in Microbiology, 13, Article 1083736. https://doi.org/10.3389/fmicb.2022.1083736
Yuan, K., Reckling, M., Artigas Ramirez, M.D., Djedidi, S., Fukuhara, I., Ohyama, T., Yokoyama, T., Bellingrath-Kimura, S.D., Halwani, M., Egamberdieva, D. and Ohkama-Ohtsu, N. (2020) Characterization of Rhizobia for the Improvement of Soybean Cultivation at Cold Conditions in Central Europe. Microbes and Environments, 35, Article ID: ME19124. https://doi.org/10.1264/jsme2.ME19124
Zhao, L.F., Xu, Y.J. and Lai, X.H. (2018) Antagonistic Endophytic Bacteria Associated with Nodules of Soybean (Glycine Max L.) and Plant Growth-Promoting Properties. Brazilian Journal of Microbiology, 49, 269-278. https://doi.org/10.1016/j.bjm.2017.06.007
Shao, S., Chen, M., Liu, W., Hu, X., Wang, E.T., Yu, S. and Li, Y. (2020) Long-Term Monoculture Reduces the Symbiotic Rhizobial Biodiversity of Peanut. Systematic and Applied Microbiology, 43, Article ID: 126101. https://doi.org/10.1016/j.syapm.2020.126101
Wang, C.Y., Michalet, R., Liu, Z., Jiang, X., Wang, X., Zhang, G., An, L., Chen, S. and Xiao, S. (2020) Disentangling Large- and Small-Scale Abiotic and Biotic Factors Shaping Soil Microbial Communities in an Alpine Cushion Plant System. Frontiers in Microbiology, 11, Article 925. https://doi.org/10.3389/fmicb.2020.00925
Sun, L., Wang, S., Narsing Rao, M.P., Shi, Y., Lian, Z.H., Jin, P.J., Wang, W., Li, Y.M., Wang, K.K., Banerjee, A., Cui, X.Y. and Wei, D. (2023) The Shift of Soil Microbial Community Induced by Cropping Sequence Affect Soil Properties and Crop Yield. Frontiers in Microbiology, 14, Article 1095688. https://doi.org/10.3389/fmicb.2023.1095688
Foley, M.M., Blazewicz, S.J., McFarlane, K.J., Greenlon, A., Hayer, M., Kimbrel, J.A., Koch, B.J., Monsaint-Queeney, V.L., Morrison, K., Morrissey, E., Hungate, B.A. and Pett-Ridge, J. (2023) Active Populations and Growth of Soil Microorganisms Are Framed by Mean Annual Precipitation in Three California Annual Grasslands. Soil Biology and Biochemistry, 177, Article ID: 108886. https://doi.org/10.1016/j.soilbio.2022.108886
Yang, H.J., Ye, W.W., Yu, Z., Shen, W.L., Li, S.Z., Wang, X., Chen, J.J., Wang, Y.C. and Zheng, X.B. (2023) Host Niche, Genotype, and Field Location Shape the Diversity and Composition of the Soybean Microbiome. Journal of Integrative Agriculture, 22, 2412-2425. https://doi.org/10.1016/j.jia.2023.01.006
Mhete, M., Eze, P.N., Rahube, T.O. and Akinyemi, F.O. (2020) Soil Properties Influence Bacterial Abundance and Diversity under Different Land-Use Regimes in Semi-Arid Environments. Scientific African, 7, e00246. https://doi.org/10.1016/j.sciaf.2019.e00246
Vázquez-Rodríguez, C., García-Olivares, J.G., Quíroz-Velasquez, J.D. and Gill-Langarica, H.R. (2023) Identificación de bacterias rizobiales asociadas al cultivo de la soya en regiones productoras al sur del estado. En: La biodiversidad en Tamaulipas. Estudio de Estado. Vol. I. CONABIO, Mexico.
Hernández-Oaxaca, D., Claro-Mendoza, K.L., Rogel, M.A., Rosenblueth, M., Velasco-Trejo, J.A., Alarcón-Gutiérrez, E., García-Pérez, J.A., Martínez-Romero, J., James, E.K. and Martínez-Romero, E. (2022) Genomic Diversity of Bradyrhizobium from the Tree Legumes Inga and Lysiloma (Caesalpinioideae-Mimosoid Clade). Diversity, 14, Article 518. https://doi.org/10.3390/d14070518
López-López, A., Negrete-Yankelevichb, S., Rogel, M.A., Ormeno-Orrillo, E., Martínez, J. and Martínez-Romeroa, E. (2013) Native Bradyrhizobia from Los Tuxtlas in Mexico Are Symbionts of Phaseolus lunatus (Lima Bean). Systematic and Applied Microbiology, 36, 33-38. https://doi.org/10.1016/j.syapm.2012.10.006
Azarias Guimarães, A., Ligiane Aparecida, F., Alves Almeida, K., Lebbe, L., Barroso Silvaa, K., Willems, A. and de Souza Moreira, F.M. (2015) High Diversity of Bradyrhizobium Strains Isolated from Several Legume Species and Land Uses in Brazilian Tropical Ecosystems. Systematic and Applied Microbiology, 38, 433-441. https://doi.org/10.1016/j.syapm.2015.06.006