Foliar Application of Chelated Selenium Modulated the Chlorophyll Metabolism, Antioxidant Capacity, and Yield of Rice under Salt Stress — Oak Academic Publishing
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Foliar Application of Chelated Selenium Modulated the Chlorophyll Metabolism, Antioxidant Capacity, and Yield of Rice under Salt Stress
Exogenous application of chelated selenium (Se) has shown potential in alleviating salt stress in crops, yet its specific effects on the yield and rice remain elusive. This study investigated the impact of foliar application of different concentrations of chelated Se i . e ., 0 (CK), 5, 10, and 15 mg L −1 denoted as T1, T2, and T3, respectively on morpho-physio-biochemical and yield attributes of two rice varieties i . e ., Meixiangzhan 2 (MXZ2) and Liangxiangyou 868 (LXY868) under salt stress (0.1%). The results demonstrated that the Se application at 10 mg/L treatment exhibited the highest yield for both rice varieties among all treatments. Physiological analyses revealed that the 10 mg L −1 treatment elevated chlorophyll content and 5-aminolevulinic acid (ALA) levels during the grain-filling stage while inhibiting the activities of chlorophyll degradation enzymes i . e ., chlorophyllase and pheophorbide an oxygenase). Moreover, Se application also modulated the net photosynthetic rates and leaf area index at grain filling stage and dry matter accumulation at maturity stage by 17.25 - 21.52%, 13.77 - 22.18%, and 14.95 - 19.01%, respectively. Furthermore, Se application at 10 mg L −1 treatment markedly improved antioxidant enzyme activities and reduced malondialdehyde (MDA) content, effectively mitigating salt stress-induced membrane damage. In conclusion, foliar application of Se at 10 mg L −1 proved to be the optimal dose for enhancing rice growth and yield under salt stress conditions.
Khan, Z., Jan, R., Asif, S., Farooq, M., Jang, Y., Kim, E., et al . (2024) Exogenous Melatonin Induces Salt and Drought Stress Tolerance in Rice by Promoting Plant Growth and Defense System. Scientific Reports , 14, Article No. 1214. https://doi.org/10.1038/s41598-024-51369-0
Zheng, C., Liu, C., Liu, L., Tan, Y., Sheng, X., Yu, D., et al . (2023) Effect of Salinity Stress on Rice Yield and Grain Quality: A Meta-Analysis. European Journal of Agronomy , 144, Article 126765. https://doi.org/10.1016/j.eja.2023.126765
Li, Q., Zhu, P., Yu, X., Xu, J. and Liu, G. (2024) Physiological and Molecular Mechanisms of Rice Tolerance to Salt and Drought Stress: Advances and Future Directions. International Journal of Molecular Sciences , 25, Article 9404. https://doi.org/10.3390/ijms25179404
Per, T.S., Khan, N.A., Reddy, P.S., Masood, A., Hasanuzzaman, M., Khan, M.I.R., et al . (2017) Approaches in Modulating Proline Metabolism in Plants for Salt and Drought Stress Tolerance: Phytohormones, Mineral Nutrients and Transgenics. Plant Physiology and Biochemistry , 115, 126-140. https://doi.org/10.1016/j.plaphy.2017.03.018
Ahmad, R., Waraich, E.A., Nawaz, F., Ashraf, M.Y. and Khalid, M. (2015) Selenium (Se) Improves Drought Tolerance in Crop Plants—A Myth or Fact? Journal of the Science of Food and Agriculture , 96, 372-380. https://doi.org/10.1002/jsfa.7231
El-Badri, A.M., Batool, M., Mohamed, I.A.A., Wang, Z., Wang, C., Tabl, K.M., et al . (2022) Mitigation of the Salinity Stress in Rapeseed ( Brassica Napus L.) Productivity by Exogenous Applications of Bio-Selenium Nanoparticles during the Early Seedling Stage. Environmental Pollution , 310, Article 119815. https://doi.org/10.1016/j.envpol.2022.119815
Luo, H., He, L., Du, B., Pan, S., Mo, Z., Duan, M., et al . (2020) Biofortification with Chelating Selenium in Fragrant Rice: Effects on Photosynthetic Rates, Aroma, Grain Quality and Yield Formation. Field Crops Research , 255, Article 107909. https://doi.org/10.1016/j.fcr.2020.107909
Qin, X., Wang, Z., Lai, J., Liang, Y. and Qian, K. (2025) The Synthesis of Selenium Nanoparticles and Their Applications in Enhancing Plant Stress Resistance: A Review. Nanomaterials , 15, Article 301. https://doi.org/10.3390/nano15040301
Ao, B., Du, Q., Liu, D., Shi, X., Tu, J. and Xia, X. (2023) A Review on Synthesis and Antibacterial Potential of Bio-Selenium Nanoparticles in the Food Industry. Frontiers in Microbiology , 14, Article 1229838. https://doi.org/10.3389/fmicb.2023.1229838
Fatahiyan, F., Najafi, F. and Shirkhani, Z. (2025) Enhancing Salt Stress Tolerance in Carthamus tinctorius L. through Selenium Soil Treatment: Anatomical, Biochemical, and Physiological Insights. BMC Plant Biology , 25, Article No. 100. https://doi.org/10.1186/s12870-025-06078-9
Liang, Y., Li, D., Chen, Y., Cheng, J., Zhao, G., Fahima, T., et al . (2020) Selenium Mitigates Salt-Induced Oxidative Stress in Durum Wheat ( Triticum durum Desf.) Seedlings by Modulating Chlorophyll Fluorescence, Osmolyte Accumulation, and Antioxidant System. 3 Biotech , 10, Article No. 368. https://doi.org/10.1007/s13205-020-02358-3
Elkelish, A.A., Soliman, M.H., Alhaithloul, H.A. and El-Esawi, M.A. (2019) Selenium Protects Wheat Seedlings against Salt Stress-Mediated Oxidative Damage by Up-Regulating Antioxidants and Osmolytes Metabolism. Plant Physiology and Biochemistry , 137, 144-153. https://doi.org/10.1016/j.plaphy.2019.02.004
Di, L. (2014) Fu An. In: Hockey, T., et al ., Eds., Biographical Encyclopedia of Astronomers , Springer New York, 767-767. https://doi.org/10.1007/978-1-4419-9917-7_488
Jiang, C., Zu, C., Lu, D., Zheng, Q., Shen, J., Wang, H., et al . (2017) Effect of Exogenous Selenium Supply on Photosynthesis, Na + Accumulation and Antioxidative Capacity of Maize ( Zea mays L.) under Salinity Stress. Scientific Reports , 7, Article No. 42039. https://doi.org/10.1038/srep42039
Gao, S., Zhou, M., Xu, J., Xu, F. and Zhang, W. (2024) The Application of Organic Selenium (SeMet) Improve the Photosynthetic Characteristics, Yield and Quality of Hybrid Rice. Plant Physiology and Biochemistry , 208, Article 108457. https://doi.org/10.1016/j.plaphy.2024.108457
Chen, Y., Dai, L., Cheng, S., Ren, Y., Deng, H., Wang, X., et al . (2024) Regulation of 2-Acetyl-1-Pyrroline and Grain Quality in Early-Season Indica Fragrant Rice by Nitrogen and Silicon Fertilization under Different Plantation Methods. Journal of Integrative Agriculture , 23, 511-535. https://doi.org/10.1016/j.jia.2023.05.009
Gu, Q., Luo, H., Lin, L., Zhang, Q., Yi, W., Liu, Z., et al . (2024) Effects of Biological Nano-Selenium on Yield, Grain Quality, Aroma, and Selenium Content of Aromatic Rice. Agronomy , 14, Article 1778. https://doi.org/10.3390/agronomy14081778
Zhang, Q., Luo, H., Xing, P., Gu, Q., Yi, W., Yu, X., et al . (2024) Responses of Hybrid Rice ( Oryza sativa L.) Plants to Different Application Modes of Nanosized Selenium. Plants , 13, Article 3179. https://doi.org/10.3390/plants13223179
Kamran, M., Parveen, A., Ahmar, S., Malik, Z., Hussain, S., Chattha, M.S., et al . (2019) An Overview of Hazardous Impacts of Soil Salinity in Crops, Tolerance Mechanisms, and Amelioration through Selenium Supplementation. International Journal of Molecular Sciences , 21, Article 148. https://doi.org/10.3390/ijms21010148
Zahedi, S.M., Karimi, M. and Teixeira da Silva, J.A. (2019) The Use of Nanotechnology to Increase Quality and Yield of Fruit Crops. Journal of the Science of Food and Agriculture , 100, 25-31. https://doi.org/10.1002/jsfa.10004
Diao, M., Ma, L., Wang, J., Cui, J., Fu, A. and Liu, H. (2014) Selenium Promotes the Growth and Photosynthesis of Tomato Seedlings under Salt Stress by Enhancing Chloroplast Antioxidant Defense System. Journal of Plant Growth Regulation , 33, 671-682. https://doi.org/10.1007/s00344-014-9416-2
Kong, L., Wang, M. and Bi, D. (2005) Selenium Modulates the Activities of Antioxidant Enzymes, Osmotic Homeostasis and Promotes the Growth of Sorrel Seedlings under Salt Stress. Plant Growth Regulation , 45, 155-163. https://doi.org/10.1007/s10725-005-1893-7
Hussain, S., Ahmed, S., Akram, W., Li, G. and Yasin, N.A. (2023) Selenium Seed Priming Enhanced the Growth of Salt-Stressed Brassica rapa L. through Improving Plant Nutrition and the Antioxidant System. Frontiers in Plant Science , 13, Article 1050359. https://doi.org/10.3389/fpls.2022.1050359
Shah, W.H., Rasool, A., Padder, S.A., Singh, R.K., Prasad, M., Tahir, I., et al . (2023) Decarboxylation Mechanisms of the C4 Cycle in Foxtail Millet Observed under Salt and Selenium Treatments. Plant Growth Regulation , 99, 65-83. https://doi.org/10.1007/s10725-022-00888-9
Wu, H., Fan, S., Gong, H. and Guo, J. (2023) Roles of Salicylic Acid in Selenium-Enhanced Salt Tolerance in Tomato Plants. Plant and Soil , 484, 569-588. https://doi.org/10.1007/s11104-022-05819-1
Sheikhalipour, M., Mohammadi, S.A., Esmaielpour, B., Spanos, A., Mahmoudi, R., Mahdavinia, G.R., et al . (2023) Seedling Nanopriming with Selenium-Chitosan Nanoparticles Mitigates the Adverse Effects of Salt Stress by Inducing Multiple Defence Pathways in Bitter Melon Plants. International Journal of Biological Macromolecules , 242, Article 124923. https://doi.org/10.1016/j.ijbiomac.2023.124923
Filek, M., Kościelniak, J., Łabanowska, M., Bednarska, E. and Bidzińska, E. (2010) Selenium-Induced Protection of Photosynthesis Activity in Rape ( Brassica napus ) Seedlings Subjected to Cadmium Stress. Fluorescence and EPR Measurements. Photosynthesis Research , 105, 27-37. https://doi.org/10.1007/s11120-010-9551-y
Lan, C., Lin, K., Huang, W. and Chen, C. (2019) Protective Effects of Selenium on Wheat Seedlings under Salt Stress. Agronomy , 9, Article 272. https://doi.org/10.3390/agronomy9060272
Liang, C., Zheng, G., Li, W., Wang, Y., Hu, B., Wang, H., et al . (2015) Melatonin Delays Leaf Senescence and Enhances Salt Stress Tolerance in Rice. Journal of Pineal Research , 59, 91-101. https://doi.org/10.1111/jpi.12243