Blackleg disease caused by Phoma lingam is an important disease of oil seed rape ( Brassica napus ) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia plymuthica HRO-C48 and Gliocladium catenulatum J1446 were able to reduce the disease intensity of OSR cotelydones infested with P. lingam at rates 44% and 52% respectively. The reduction of the infestation of the root collar in BBCH14/15 was evaluated as well. Plants treated with a suspension of the antagonists (2 × 105 cfu/plant) and inoculated with either pycnidiospore suspension (2 × 107 cfu/ml) or agar disks grown with P. lingam mycelium, showed a reduced infestation rate of 53% - 93% in the presence of S. plymuthica and 46% - 77% in the presence of G. catenulatum . The efficacy of the antagonist depends highly on their concentration inside OSR seeds. Below 105 cfu/seed no significant difference was recorded between control untreated and infested plants.
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