Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds
Biotechnology and Bioinformatics Division;
,
Biotechnology and Bioinformatics Division;
,
Phytochemistry and Phytopharmacology Division Jawaharlal Nehru Tropical Botanic Garden and Research Institute Palode, Thiruvananthapuram, Kerala 695 562, India;
,
Department of Plant Science Central University, Kasargod Kerala 671 123, India.
1 Biotechnology and Bioinformatics Division;
2 Biotechnology and Bioinformatics Division;
3 Phytochemistry and Phytopharmacology Division Jawaharlal Nehru Tropical Botanic Garden and Research Institute Palode, Thiruvananthapuram, Kerala 695 562, India;
4 Department of Plant Science Central University, Kasargod Kerala 671 123, India.
An efficient and improved clonal propagation of Alpinia calcarata , a commercially important medicinal plant was established on Murashige and Skoog medium. The axillary shoot proliferation was achieved with maximum 5.2 ± 0.7 shoots in 92.8% of rhizome explants in medium with 2.0 mg/L 6-benzylamiopurine (BAP) and 0.2 mg/L indole-3-acetic acid (IAA). Axillary shoot buds (60%) upon subculture for 8 weeks in the same medium produced multiple shoot initials (12.1 ± 0.4) mediated with meristemoids (4.0 ± 0.5) and callus. A gradual reduction in the concentration of BAP or elimination of IAA was required for rapid induction of normal plants devoid of callus from propagules during subsequent subculture. Single clump of 3-4 multiple shoot initials during second subculture on medium with 1.0 mg/L BAP and 0.1 mg/L IAA yielded an average of 21 shoots which was best among different propagules tried. The shoot multiplication rate was further enhanced to 32 shoots when the similar propagules passed to third subculture on medium with 1.0 mg/L BAP alone. Clumps of multiple shoot initials upon subculture on medium with 1.0 mg/L BAP alone exhibited 10 fold multiplication rates. Use of liquid medium in culture bottles with polypropylene caps supported fast growth of the shoots and spontaneous root formation on 50% of the shoots. Shoots transferred to half-strength MS liquid medium with 0.2 mg/L of IAA and IBA was optimum for maximum roots (8.14 ± 1.34) in 100% shoots. The rooted plants were hardened in mist chamber showed 95% survival and well established in the field. The acclimatized plants showed rhizome formation after 4-6 weeks of growth under shade house. Volatile chemicals profile of the leaves, rhizome and root of the in vitro and conventionally propagated plants analyzed by gas chromatography-mass spectrometry were qualitatively and quantitatively similar. The analysis of growth characteristics of 36 month old in vitro and conventionally propagated plants showed a 50% increment of rhizome fresh biomass with prolific root and leaf growth in the former than the latter ones. The protocol described herein will have practical applications for the large scale production of uniform efficient plants for commercial cultivation of A. calcarata .
J. K. Mangaly and M. Sabu, “A Taxonomic revision of South Indian Alpinia Roxb. (Zingiberaceae),” Rheedea, Vol. 2, No. 1, 1992, pp. 38-51.
J. P. Robinson, V. Balakrishnan, S. Raj and S. J. Britto, “Antimicrobial activity of Alpinia calcarata Rosc. and characterization of new α, β-unsaturated carbonyl compound,” Advances in Biological Research, Vol. 3, No. 5-6, 2009, pp. 185-187.
L. S. R. Arambewela, L. D. A. M. Araw-wawala and W. D. Ratnasoorya, “Antinoceceptive activities of aqueous and ethanolic extract of Alpinia calcarata rhizomes in rats,” Journal of Ethnopharmacology, Vol. 95, No.2-3, 2004, pp.311-316.
L. D. A. M. Arawwawala, L. S. R. Arambewela, W. D. Ratnasooriya. “Alpinia calcarata Roscoe: A potent antiinflammatory agent”, Journal of Ethnopharmacology, Vol. 139, No. 3, 2012, pp. 889-892.
T. R. Dutta, R. Ahemed, S. R. Abbas and M. K. V. Rao, “Plants used by Andaman ab-origins in gathering rock honey,” Economic Botany, Vol. 39, No. 2, 1985, pp. 130 -138.
M. Sabu, “Zingibe-raceae and Costaceae of south India,” Indian Association of Angiosperm Taxonomists, Calicut, India, 2006, p.52.
P. N. Kaul, R. B. R. Rajeswara, K. Singh, A. K. Bhattacharya, G. R. Mallavarapu and S. Ramesh, “Volatile constituents of essential oils isolated from different parts of Alpinia calcarata Rosc.,” Journal of Essential Oil Research, Vol. 17, No. 1, 2005, pp. 7-9.
N. Sasidharan and P. K. Muraleedhara, “Survey on the commercial exploitation and consumption of medicinal plants by the drug industry in northern Kerala,”, Research Report No.193, ISBN 0970-8103, Kerala Forest Research Institute, Thrissur, Kerala 2000.
S. Mohanty, R. Parida, S. Singh, R. K. Joshi, E. Subudhi and S. Nayak, “Biochemical and molecular profiling of micropropagated and conventionally grown Kaempferia galanga,” Plant Cell Tissue and Organ Culture, Vol. 106, No.1, 2010, pp. 39-46.
M. Singh and R. Chaturvedi, “Improved clonal propagation of Splilanthes acmella Murr. for production of scopoletin,” Plant Cell Tissue and Organ Culture, Vol. 103, No. 2, 2010, pp. 243-253. doi:10.1007/s 11240-010-9774-9
M. S. Rathore and N. S. Shekhawat, “Micropropagation of Pueraria tuberose (Roxb. Ex Willd.) and determination of puerarin content in different tissues,” Plant Cell Tissue and Organ Culture, Vol. 99, No. 3, 2009, pp. 327-334.
P. K. Pati, J. Kaur and P. Singh, “A liquid culture system for shoot proliferation and analysis of pharmaceutically active constituents of Catharanthus roseus (L.) G. Don.,” Plant Cell Tissue and Organ Culture, Vol. 105, No.3, pp. 299-307.
GC-MS
K. T. Agretious, K. P. Martin and M. Ha-riharan, “In vitro clonal multiplication of Alpinia calcarata Rosc.,” Phytomorphology, Vol. 46, No. 2, 1996, pp. 133-138.
T. Murashige and F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue culture,” Physiologia Plantarum, Vol. 15, No. 3, 1962, pp. 473-497.
H. Van Den Dool and P. D. Kratz, “A generalization of the retention index system including linear temperature programmed gas liquid partition Chromatography,” Journal of Chromatography, Vol. 11: August 1963, pp. 463-471.
R. P. Adams, “Identification of essential oil components by gas chromatography/ mass spectrometry”, 4th edn., Allured Pub. Co., Carol Stream, IL., 2007.
M. Borthakur, J. Hazarika and R. S. Sing, “A protocol for micropropagation of Alpinia galangal,” Plant Cell Tissue and Organ Culture, Vol. 55, No. 3, 1999, pp. 231-233.
N. H. Loc, D. T. Duc, T. H. Kwon and M. S. Yang, “Micropropagation of Zedoary (Curcuma zedoaria Roscoe)-a valuable medicinal plant,” Plant Cell Tissue and Organ Culture, Vol. 81, No. 1, 2005, pp. 119-122.
K. K. Behera, D. Pani and S. Sahoo, “Effect of plant growth regulator on in vitro multiplication of turmeric (Curcuma longa L cv. Ranga),” International Journal of Biological Technology, Vol. 1, No. 1, 2010, pp. 16-23.
S. K. Shukla, S. Shukla, V. Koche and S. K. Mishra, “In vitro propagation of tikhur (Curcuma angustifolia Roxb.): A starch yielding plant,” Indian Journal of Biotechnology, Vol. 6, April 2007, pp. 274-276.
S. M. Balachandran, S. R. Bhat and K. P. S. Chandel, “In vitro clonal multiplication of turmeric (Curcuma spp) and ginger (Zingiber officinale Rosc.),” Plant Cell Reports, Vol. 8, No. 9, January 1990, pp. 521-524.
S. P. Geetha, C. Manjula, C. Z. John, D. Minoo, B. K. Nirmal and P. N. Ravindran, “Micropropagation of Kaempferia spp. (K.galanga L and K.rotunda L.),” Journal of Spices and Aromatic Crops, Vol. 6, No. 2, 1997, pp. 129-135.
K. Nasirujjaman, M. S. Uddin, S. Zaman and M. A. Reza, “Micropropagaion of turmeric (Curcuma longa Linn.) through in vitro rhizome bud culture,”. Journal of Biological Sciences, Vol. 5, No. 4, 2005, pp. 490-492.
S. Prathanturarug, N. Soonthornchareonnon, W. Chuakul, Y. Phaidee and P. Saralamp, “Rapid micropropagation of Curcuma longa using bud explants precultured in thidiazuron-supplemented liquid medium,” Plant Cell Tissue and Organ Culture, Vol. 80, No. 3, 2005, pp. 347-351.
T. R. Sharma and B. M. Singh, “High frequency in vitro multiplication of disease-free Zingiber officinale Rosc.,” Plant Cell Reports, Vol. 17, No.1, 1997, pp. 68-72.
S. Nayak, T. Kaur, S. Mohanty, G. Ghosh, R. Choudhury, L. Acharya and E. Subudhi, “In vitro and ex vitro evaluation of long-term micropropagated turmeric as analysed through cytophotometry, phytoconsituents, biochemical and molecular markers,” Plant Growth Regulation, Vol. 64, No.1, 2010, pp.91-98.
M. S. Rathore and N. S. Shekhawat,) “Micropropagation of Pueraria tuberose (Roxb. Ex Willd.) and determination of puerarin content in different tissues”. Plant Cell Tissue and Organ Culture, 69: No. 4 2009, pp. 327-33
K. T. Kuppusamy, C. L. Walcher and J. L. Nemhauser, “Cross regulatory mechanism in hormone signaling,” Plant Molecular Biology, Vol. 69, No. 4, 2009, pp. 375-381.
M. Bejoy, M. Dan and M. P. Anish, “Factors affecting the in vitro multiplication of the endemic Zingiber Curcuma haritha Mangaly and Sabu,”. Asian Journal of Plant Sciences, Vol. 5, No. 5, 2006, pp. 847-853.
R. Parida, S. Mohanty, A. Kuanar and S. Nayak, “Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture,” Electronic Journal of Biotechnology, Vol. 13, No. 4, 2010, fulltext-12,
F. L. Marga, R. N. Prasad and A. Roychowdhury, “Agar fraction could protect apple shoots cultured in liquid medium against hyperhydricity,” Plant Cell Tissue and Organ Culture, Vol. 49, No. 1, 1997, pp. 1-5.
C. Stanly, A. Bhatt and C. L. Keng, “A comparative study of Curcuma zedoaria and Zingiber zerumbet plantlet production using different micropropagation systems,” African Journal of Biotechnology, Vol. 9, No. 28, July 2010, pp. 4326-4333.
N. D. Salvi, L. George and S. Eapen, “Micropropagation and field evaluation of micropropagated plants of turmeric,” Plant Cell Tissue and Organ Culture, Vol. 68, No. 2, 2002, pp. 143-151.
I. M. Fortunato and P. Avato, “Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. hirtum (Link) Ietswaart,” Plant Cell Tissue and Organ Culture, Vol. 93, No. 2, 2008, pp. 139-149.