Research ArticleOpen AccessGoogle Scholar indexed
Genetic Transformation Studies on Avocado Cultivar “Hass” (Persea americana)
University of Western Sydney, South Penrith DC, Australia
Department of Agricultural Sciences, American University of Beirut, Beirut, Lebanon
University of Western Sydney, South Penrith DC, Australia
- 1 University of Western Sydney, South Penrith DC, Australia
- 2 Department of Agricultural Sciences, American University of Beirut, Beirut, Lebanon
- 3 University of Western Sydney, South Penrith DC, Australia
American Journal of Plant Sciences·Volume 03 (2012)·Pages 1225–1231·Published 26 September 2012·DOI10.4236/ajps.2012.39148
Copy link · social · email
Abstract
The use of traditional breeding for improvement of avocado cultivars is time consuming, hence other methods such as genetic transformation by Agrobacterium is indispensable to adopt. The strain GV3850/pBI121gave best transformation outcome compared to five other binary vectors (AGL1/pCGP904; AGL1/pBI121; GV3850/pCGP904; LBA4404/pCG-P904 and LBA4404/pBI121) under different pH and acetosyringone concentrations. The optimal condition for reliable transformation was by using 200 μM acetosyringone and a pH of 5.2. Transformed embryonic shoots co-cultivated with GV3850/pBI121 were tested using the histochemical x-gluc assay. Further analysis was conducted by polymerase chain reaction using specific primers for the reporter gene (GUS).
KeywordsAvocadoPerseaBinary VectorsGUS Reporter
- R. B. Horsch, J. Fry, N. Hoffmann, J. Neidermeyer, S. G. Rogers and R. T. Fraley, “Leaf Disc Transformation,” In: S. B. Gelvin, R. A. Schilperoort and D. P. Verma, Eds., Plant Molecular Biology Manual, Kluwer Academic Publisher, Belgium, 1988, pp. 1-9.
- H. Klee and S. Rogers, “Plant Gene Vectors and Genetic Transformation: Plant Transformation System Based on the Use of Agrobacterium tumefaciens,” In: J. Schell and I. K. Vasil, Eds., Cell Culture and Somatic Cell Genetics of Plants, Vol. 6: Molecular Biology of Plant Nuclear Genes, Academic Press, London, 1989, pp. 1-23.
- G. An, P. R. Ebert, A. Mitra and S. Ha, “Binary Vectors,” In: S. B. Gelvin, R. A. Schilperoort and D. P. Verma, Eds., Plant Molecular Biology Manual, Kluwer Academic Publisher, Belgium, 1988, pp. 1-19.
- P. Zambryski, H. Joos, C. Genetello, J. Leemans, M. Van Montagu and J. Schell, “Ti-Plasmid Vector for the Introduction of DNA into Plant Cells without Alteration of Their Normal Regeneration Capacity,” EMBO Journal, Vol. 12, No. 2, 1983, pp. 2143-2150.
- A. Hoekema, P. R. Hirsch, P. J. J. Hookaas, and R. A. Schilperoot, “A Binary Plant Vector Strategy Based on Separation of vir- and T-region of the Agrobacterium tumefaciens Ti Plasmid,” Nature, Vol. 303, 1983, pp. 179-180. doi:10.1038/303179a0
- G. Ooms, P. Hooykaas, G. Moolenaar, and R. Schilperoort, “Crown Gall Tumors of Abnormal Morphology Induced by Agrobacterium tumefaciens Carrying Mutated Octopine Ti Plasmids; Analysis of T-DNA Functions,” Gene, Vol. 14, No. 1-2, 1981, pp. 33-50. doi:10.1016/0378-1119(81)90146-3
- A. Cruz-Hernández, Witjaksono, R. E. Litz and M. Gomez Lim, “Agrobacterium tumefaciens—Mediated Transformation of Embryogenic Avocado Cultures and Regeneration of Somatic Embryos,” Plant Cell Reports, Vol. 17, No. 6-7, 1998, pp. 497-503. doi:10.1007/s002990050431
- T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 15, No. 3, 1962, pp. 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
- R. A. Jefferson, T. A. Kavanagh, and M. W. Bevan, “GUS Fusions: β-Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in Higher Plants,” EMBO Journal, Vol. 6, No. 13, 1987, pp. 3901-3907.
- K. H. Lipp Joao and T. A. Brown, “Enhanced Transformation of Tomato Co-Cultivated with Agrobacterium tumefaciens C58CIRif::pGSFR1161 in the Presence of Acetosyringone,” Plant Cell Reports, Vol. 12, No. 7-8, 1993, pp. 422-425. doi:10.1007/BF00234705