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Effective Procedure for Development of EST-SSR Markers Using cDNA Library
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea (South)
Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea (South)
School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea (South)
School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea (South)
- 1 Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea (South)
- 2 Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea (South)
- 3 School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea (South)
- 4 School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea (South)
American Journal of Plant Sciences·Volume 03 (2012)·Pages 1322–1327·Published 26 September 2012·DOI10.4236/ajps.2012.39159
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Abstract
The present study was conducted to develop EST-SSR markers using the cDNA library from rice plant. Total RNA extracted from the leaves of brown plant hopper resistance gene originated from a rice cultivar “Cheongcheong” and sensitive rice cultivar “Nakdong” were used to synthesize a cDNA library. As a result of analyzing the cDNA library, the 17 EST-SSR primer sets were developed. This study enables to provide effective marker assisted selection (MAS) methods on the selection of white-backed planthopper resistance gene originated from a rice plant more simply, quickly and precisely. Furthermore, using this marker’s advantage of deriving from cDNA, it is possible to identify the white-backed planthopper resistance gene. In addition, this study introduces a technique for construction of a cDNA library safely without using radioactivity.
KeywordscDNAEST-SSR MarkersRadioactivityRice
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