Exogenous Application of Salicylic Acid and Nitric Oxide on the Ionic Contents and Enzymatic Activities in NaCl-Stressed Soybean Plants — Oak Academic Publishing
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Exogenous Application of Salicylic Acid and Nitric Oxide on the Ionic Contents and Enzymatic Activities in NaCl-Stressed Soybean Plants
Department of Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran
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Department of Biology, Research and Science Branch, Islamic Azad University, Tehran, Iran
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Faculty of Biological Sciences, Shahid Beheshti G. C. University, Tehran, Iran
1 Department of Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran
2 Department of Biology, Research and Science Branch, Islamic Azad University, Tehran, Iran
3 Faculty of Biological Sciences, Shahid Beheshti G. C. University, Tehran, Iran
The possible protective role of exogenous SA and NO donor (sodium nitroprusside) against salt stress was examined NaCl-induced oxidative stress in Glycine max L. The results indicated that NaCl-induced ionic toxicity led to significant increase of Na + uptake in root and shoot of soybean, while K + and Ca 2+ uptake was decreased markedly. Application of SA, SNP and the combination of SA with SNP inhibited Na+ uptake, but improved K + and Ca 2+ uptake. Under NaCl salinity, germination percentage (GP) was decreased significantly as compared with control plants. Moreover, the activity of lipoxygenase (LOX) was increased by NaCl, while the application of SA, NO and SA + SNP could decrease LOX activity in this condition. As well, activities of the polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were significantly decreased by 100 mM NaCl, while the application of exogenous SA and NO increased the activities of PPO and PAL. In addition, it was observed that the SA and NO have effective functions in decreasing the damages induced by NaCl salinity. The seeds of G. max cv. Union × Elf (called L17) were obtained from the Institute of Sapling and Seed in Tehran and experiments have been done in Science and Research Branch, Islamic Azad University. After treatment with SA, SNP and NaCl, the plants were harvested for the estimation of ionic contents and assay of enzyme activities. The results showed that SA and NO act synergistically to reduce the damaging effects of salt tress via enhancing the activity of antioxidative systems.
M. Sharifi, M. Ghorbanli and E. Ebrahimzadeh, “Improved Growth of Salinity Stressed Soybean after Inoculation with Salt Pre-Treated Mycorrhizal Fungi,” Journal of Plant Physiology, Vol. 164, No. 9, 2007, pp. 1144-1151. doi:10.1016/j.jplph.2006.06.016
M. Simaei, R. A. Khavari-Nejad, S. Saadatmand, F. Bernard and F. Fahimi, “Interactive Effects of Salicylic Acid and Nitric Oxide on Soybean Plants under NaCl Salinity,” Russian Journal of Plant Physiology, Vol. 58, No. 5, 2011, pp. 783-390. doi:10.1134/S1021443711050220
E. Horvath, G. Szalai and T. Janda, “Induction of Abiotic Stress Tolerance by Salicylic Acid Signaling,” Journal of Plant Growth Regulation, Vol. 26, No. 3, 2007, pp. 290-300. doi:10.1007/s00344-007-9017-4
I. Raskin, “Role of Salicylic Acid in Plants,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 43, 1992, pp. 439-463. doi:10.1146/annurev.pp.43.060192.002255
A. Metwally, I. Finkemeier, M. Georgi and K. J. Dietz, “Salicylic Acid Alleviates the Cadmium Toxicity in barley Seedlings,” Plant Physiology, Vol. 132, No. 1, 2003, pp. 272-281. doi:10.1104/pp.102.018457
M. Waseem, H. R Athar and M. Ashraf, “Effect of Salicylic Acid Applied through Rooting Medium on Drought Tolerance of Wheat,” Pakistan Journal of Botany, Vol. 38, No. 4, 2006, pp. 1127-1136.
M. Arfan, H. R. Athar and M. Ashraf, “Does Exogenous Application of Salicylic Acid through the Rooting Medium Modulate Growth and Photosynthetic Capacity in Two Differently Adapted Spring Wheat Cultivars under Salt Stress?” Journal of Plant Physiology, Vol. 164, No. 6, 2007, pp. 685-694. doi:10.1016/j.jplph.2006.05.010
M. Arasimowicz and J. Floryszak-Wieczorek, “Nitric Oxide as a Bioactive Signaling Molecule in Plant Stress Responses,” Plant Science, Vol. 172, No. 5, 2007, pp. 876-887. doi:10.1016/j.plantsci.2007.02.005
A. I. Lopez-Carrion, R. Castellano, M. A. Rosales, J. M. Ruiz and L. Romero, “Role of Nitric Oxide under Saline Stress: Implications on Proline Metabolism,” Biologia Plantarum, Vol. 52, No. 3, 2008, pp. 587-591. doi:10.1007/s10535-008-0117-1
L. Lamattina, C. Garcia-Mata, M. Graziano and G. Pagnussat, “Nitric Oxide: The Versatility of an Extensive Signal Molecule,” Annual Review of Plant Biology, Vol. 54, 2003, pp. 109-136. doi:10.1146/annurev.arplant.54.031902.134752
D. R. Hoagland and D. I. Arnon, “The Water-Culture for Growing Plants without Soil,” Agricultural Experiment Station Circulation, Vol. 374, 1957.
J. D. Maguire, “Speed of Germination-Aid in Selection and Evaluation for Seedling Emergence and Vigor,” Crop Science, Vol. 2, No. 2, 1962, pp. 176-177. doi:10.2135/cropsci1962.0011183X000200020033x
D. T. Krizek, S. J. Britz and R. M. Mirecki, “Inhibitory Effects of Ambient Levels of Solar UV-A and UV-B Radiation on Growth of cv. New Red Fire Lettuce,” Physiologia Plantarum, Vol. 103, No. 1, 1998, pp. 1-7.
G. J. Wagner, “Content and Vacuole/Extra Vacuole Distribution of Neutral Sugars, Free Amino Acids, and Anthocyanins in Protoplast,” Plant Physiology, Vol. 64, No. 1, 1979, pp. 88-93. doi:10.1104/pp.64.1.88
M. M. Bradford, “A Rapid and Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,” Analytical Biochemistry, Vol. 72, No. 1-2, 1976, pp. 248-254.
A. Doderer, I. Kokkelink, S. Van der Veen, B. Valk, A. Schram and A. Douma, “Purification and Characterization of Two Lipoxygenase Isoenzymes from Germinating Barley,” Biochimica et Biophysica Acta (BBA), Vol. 1120, No. 1, 1992, pp. 97-104. doi:10.1016/0167-4838(92)90429-H
C. N. Giannopolitis and S. K. Ries, “Superoxide Dismutases. I. Occurrence in Higher Plants,” Plant Physiology, Vol. 59, No. 2, 1977, pp. 309-314. doi:10.1104/pp.59.2.309
J. Raymond, N. Rakariyatham and J. L. Azanza, “Purification and Some Properties of Polyphenoloxidase from Sunflower Seeds,” Phytochemistry, Vol. 34, No. 4, 1993, pp. 927-931. doi:10.1016/S0031-9422(00)90689-7
N. Ochoa-Alejo and J. E. Gomez-Peralta, “Activity of Enzymes Involved in Capsaicin Biosynthesis in Callus Tissue and Fruits of Chili Pepper (Capsicum annuum L.),” Journal of Plant Physiology, Vol. 141, No. 2, 1993, pp. 147-152. doi:10.1016/S0176-1617(11)80751-0
V. Chinnusamy, J. Zhu and J. K. Zhu, “Salt Stress Signaling and Mechanisms of Plant Salt Tolerance,” Genetic Engineering, Vol. 27, 2006, pp. 141-177. doi:10.1007/0-387-25856-6_9
J. K. Zhu, J. P. Liu and L. M. Xiong, “Genetic Analysis of Salt Tolerance in Arabidopsis: Evidence for a Critical Role of Potassium Nutrition,” The Plant Cell, Vol. 10, No. 7, 1998, pp. 1181-1191.
R. Serrano, J. M. Mulet, G. Rios, J. A. Marquez, I. F. de Larriona, M. P. Leube, I. Mendizabal, A. Pascual-Ahuir, M. R. R. Proft and C. Montesinos, “A Glimpse of the Mechanism of Ion Homeostasis during Salt Stress,” Journal of Experimental Botany, Vol. 50, Special issue, 1999, pp. 1023-1036.
Y. Guo, Z. Tian, D. Yan, J. Zhang, P. Qin, “Effects of Nitric Oxide on Salt Stress Tolerance in Kosteletzkya virginica,” Life Science Journal, Vol. 6, 2009, pp. 67-75.
M. G. Zhao, Q. Y. Tian and W. H. Zhang, “Nitric Oxide Synthase-Dependent Nitric Oxide Production Is Associated with Salt Tolerance in Arabidopsis,” Plant Physiology, Vol. 144, No. 1, 2007, pp. 206-217. doi:10.1104/pp.107.096842
Y. Y. Zhang, J. Liu, Y. L. Liu, “Nitric Oxide Alleviates the Growth Inhibition of Maize Seedlings under Salt Stress,” Journal of Plant Physiology and Molecular Biology, Vol. 30, No. 4, 2004, pp. 455-459.
A. M. A. Al-Hakimi, “Effect of Salicylic Acid on Biochemical Changes in Wheat Plants under Khat Leaves Residues,” Plant, Soil and Environment, Vol. 54, No. 7, 2008, pp. 288-293.
A. C. Scott, N. S. Allen, “Changes in Cytosolic pH within Arabidopsis Root Columella Cells Play a Key Role in the Early Signaling Pathway for Root Gravitropism,” Plant Physiology, Vol. 121, No. 4, 1999, pp. 1291-1298. doi:10.1104/pp.121.4.1291
L. Rubio, A. Rosado, A. Linares-Rueda, O. Borsani, M. J. Garcia-Sanchez, V. Valpuesta, J. A. Fernandez, M. A. Botella, “Regulation of K+ Transport in Tomato Roots by the TSS1 Locus, Implications in Salt Tolerance,” Plant Physiology, Vol. 134, No. 1, 2004, pp. 452-459. doi:10.1104/pp.103.030361
F. M. Shakirova and D. R. Sahabutdinova, “Changes in the Hormonal Status of Wheat Seedlings Induced by Salicylic Acid and Salinity,” Plant Science, Vol. 164, No. 3, 2003, pp. 317-322. doi:10.1016/S0168-9452(02)00415-6
P. C. Bethke, I. G. L. Libourel, V. Reinohl and R. L. Jones, “Sodium Nitroprusside, Cyanide, Nitrite, and Nitrate Break Arabidopsis Seed Dormancy in a Nitric Oxide-Dependent Manner,” Planta, Vol. 223, No. 4, 2006, pp. 805-812.
G. Schaller and J. Kieber, “Ethylene,” American Society of Plant Biologists, 2002, pp. 1-17.
L. Popova, T. Pancheva and A. Uzunova, “Salicylic Acid: Properties, Biosynthesis and Physiological Role,” Bulgarian Journal of Plant Physiology, Vol. 23, 1997, pp. 85-93.
H. S. Chae, F. Faure and J. J. Kieber, “The eto1, eto2, and eto3 Mutations and Cytokinin Treatment Increase Ethylene Biosynthesis in Arabidopsis by Increasing the Stability of ACS Protein,” The Plant Cell, Vol. 15, No. 2, 2003, pp. 545-559. doi:10.1105/tpc.006882
A. Molassiotis, G. Tanoug, A. Patakas, “Effects of 4-Month Fe Deficiency Exposure on Fe2+ Reduction Mechanism, Photosynthetic Gas Exchange Chlorophyll Fluorescence and Antioxidant Defense in Two Peach Rootstocks Differing in Fe2+ Deficiency Tolerance,” Journal of Plant Nutrition, Vol. 25, 2005, pp. 843-860.
M. V. Beligni, A. Fath, P. C. Bethke, L. Lamattina and R. L. Jones, “Nitric Oxide Acts as an Antioxidant and Delays Programmed Cell Death in Barley Aleurone Layers,” Plant Physiology, Vol. 129, 2002, pp. 1642-1650. doi:10.1104/pp.002337
C. Sudhakar, A. Lakshmi and S. Giridarakumar, “Changes in Antioxidant Enzyme Efficacy in Two High Yielding Genotypes of Mulberry (Morus alba L.) under NaCl Salinity,” Plant Science, Vol. 61, 2001, pp. 613-619 doi:10.1016/S0168-9452(01)00450-2
R. ,Haihua, S. Wenbiao, Y. E. Maobing and X. U. Langlai, Protective Effects of Nitric Oxide on Salt Stress-Induced Oxidative Damage to Wheat (Triticum aestivum L.) Leaves,” Chinese Science Bullitin, Vol. 47, 2002, pp. 677-681.
A. Chandra and R. Saxena, “Change in Phenylalanine Ammonia Lyase Activity and Isozyme Patterns of Polyphenol Oxidase and Peroxidase by Salicylic Acid Leading to Enhance Resistance in Cowpea against Rhizoctonia solani,” Acta Physiologiae Plantarum, Vol. 29, No. 4, 2007, pp. 361-367. doi:10.1007/s11738-007-0045-2
J. Durner, D. Wendehenne and D. F. Klessig, “Defense Gene Induction in Tobacco by Nitric Oxide, Cyclic GMP, and Cyclic ADP-Ribose,” Proceedings of the National Academy of Sciences, Vol. 95, No. 17, 1998, pp. 10328-10333. doi:10.1073/pnas.95.17.10328
G. L. Shadle, S. V. Wesley, K. L. Korth, F. Chen, C. Lamb and R. A. Dixon, “Phenylpropanoid Compounds and Disease Resistance in Transgenic Tobacco with Altered Expression of L-Phenylalanine Ammonia-Lyase,” Phytochemistry, Vol. 64, No. 1, 2003, pp. 153-161. doi:10.1016/S0031-9422(03)00151-1