Protein Profiles and Dehydrin Accumulation in Some Soybean Varieties (<i>Glycine max</i> L. Merr) in Drought Stress Conditions — Oak Academic Publishing
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Protein Profiles and Dehydrin Accumulation in Some Soybean Varieties (<i>Glycine max</i> L. Merr) in Drought Stress Conditions
Biology Department, Faculty of Science, University of Brawijaya, Malang, Indonesia
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Biology Department, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang, Malang, Indonesia
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Research Institute of Legume and Tuber Crops, Malang, Indonesia
1 Biology Department, Faculty of Science, University of Brawijaya, Malang, Indonesia
2 Biology Department, Faculty of Science and Technology, Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang, Malang, Indonesia
3 Research Institute of Legume and Tuber Crops, Malang, Indonesia
Drought is one of environmental stresses which the most limiting to plant growth and productivity. Drought stress led to a series of changes including biochemical changes like accumulation of osmolit and specific proteins involved in stress tolerance. One of the proteins that play a role in the mechanism of drought resistance is dehydrin protein. This study aimed to identify the protein profiles and dehydrin accumulation in 7 varieties of local Indonesia n soybean s : Tanggamus, Nanti, Seulawah and Tidar (tolerant), Wilis and Burangrang (moderate) and Detam - 1 (drought stress sensitive). Plants were treated with drought stress by adjusting soil water content to 25% below field capacity and compared with plants which were grown on normal condition as control plants. The results of SDS-PAGE electrophoresis showed a new protein with the molecular weight of 13 and 52 kDa were induced in Tanggamus, Nanti , Seulawah and Tidar varieties . W estern blotting analysis for dehydrin showed that the quantity of the protein in the leaves of all varieties except Tanggamus decreased in drought stress conditions. The quantity of d ehydrin protein in tolerant varieties higher than the protein quantity in both moderate varieties and drought sensitive.
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