Changes in Inositol Phosphates in Low Phytic Acid Field Pea (<i>Pisum sativum</i> L.) Lines during Germination and in Response to Fertilization — Oak Academic Publishing
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Changes in Inositol Phosphates in Low Phytic Acid Field Pea (<i>Pisum sativum</i> L.) Lines during Germination and in Response to Fertilization
School of Food Systems, North Dakota State University, Fargo, USA
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School of Food Systems, North Dakota State University, Fargo, USA
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School of Food Systems, North Dakota State University, Fargo, USA
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School of Food Systems, North Dakota State University, Fargo, USA
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School of Food Systems, North Dakota State University, Fargo, USA
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School of Food Systems, North Dakota State University, Fargo, USA
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Crop Development Centre, University of Saskatchewan, Saskatoon, Canada
1 School of Food Systems, North Dakota State University, Fargo, USA
2 School of Food Systems, North Dakota State University, Fargo, USA
3 School of Food Systems, North Dakota State University, Fargo, USA
4 School of Food Systems, North Dakota State University, Fargo, USA
5 School of Food Systems, North Dakota State University, Fargo, USA
6 School of Food Systems, North Dakota State University, Fargo, USA
7 Crop Development Centre, University of Saskatchewan, Saskatoon, Canada
Inositol phosphates are the main form of phosphorous (P) storage in legume seeds. Mutants low in inositol hexaphos phate (IP6), also known as phytic acid (PA), have been developed to increase iron (Fe) bioavailability and reduce P wast e to the environment. The objectives of this study were to determine 1) inositol-P form changes during germina tion, and 2) the effect of P fertilizer application on seed PA, total P, and Fe concentration of three field pea ( Pisum sa tivum L.) cultivars and two low - PA lines grown under greenhouse conditions. Low - PA field pea lines clearly had lower PA (1.3 - 1.4 mg·g -1 ) than cultivars (3.1 - 3.7 mg·g - 1 ). Phytic acid concentration in both cultivars and low-PA lines de creased during germination, but tended to increase seven days after germination. Levels of inositol-3-phosphate-phos phate (IP3-P; 0.6 mg·g - 1 ) and inorganic P (1.8 - 2.0 mg·g - 1 ) were higher in low-PA lines than in the field pea cultivars. Reduction of PA in low-PA line seeds may reduce seed Fe and total P concentrations, as levels in the low-PA lines (37 - 42 mg·kg - 1 Fe; 4003 - 4473 mg·kg - 1 total P) were typically less than in field pea cultivars (37 - 55 mg·kg - 1 Fe; 3208 - 4985 mg·kg - 1 total P) at different P fertilizer rates. Overall, IP3 is the major form of P present in low-PA field pea lines during germination; however IP6 is the major form of P present in field pea cultivars. Therefore, low-PA field pea lines could be a potential solution to increase Fe bioavailability, feed P utilization, and reduce P waste to the environment.
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