Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Functional Analysis of a Wilt Fungus Inducible <i>PR</i>10-1 Gene from Cotton
Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
,
Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
,
Plant Genomics Research Center, Mianyang Normal University, Mianyang, China
,
Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
,
Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
,
Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
1 Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
2 Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
3 Plant Genomics Research Center, Mianyang Normal University, Mianyang, China
4 Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
5 Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
6 Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiao Tong University, Shanghai, China
Early stage expression of PR 10 combined with phytoalexins contributed to Verticillium wilt resistance in cotton. In order to analysis the activities of PR 10 proteins during pathogens’ infection, we cloned a Verticillium -induced PR 10 ( GbPR 10-1) gene from cotton ( Gossypium barbadense ) and compared its expression patterns and domains with other PR 10 proteins. Bioinformatics indicated that GbPR 10-1 showed the lowest similarity with other 12 different PR 10 genes in cotton (Upland and sea-island cotton). Expression profiles showed that GbPR 10-1 gene instantly up-regulated after infection by V . dahliae in the sea-island cotton plants. GbPR 10-1 was also induced by environmental stimulus including heat, submergence and salt, and ethylene but not by ABA and salicylic acid. The GbPR 10-1 protein expressed in E . coli BL21 demonstrated that it had a low ribonuclease-like activity in vitro , and could inhibit V . dahliae hyphae growth but not its spores. Comparison analysis of GbPR 10-1 (from resistant species) and GhPR 10-1 (from susceptible species) responding to V . dahliae infection, only GbPR 10-1 gene was strongly induced in the sea-island cotton plants (incompatible response), indicating that PR 10-1 genes was linked to resistance signal. In summary, the earlier activation of GbPR 10-1 gene, as the index of resistance response, would be aid to block the
A. A. Bell, “Mechanisms of Disease Resistance in Gossypium Species and Variation in Verticillium dahliae,” In: G. A. Constable and N. W. Forrester, Eds., Challenging the Future, Proceedings of the World Cotton Research Conference, 1-5 February 1995, Commonwealth Scientific and Industrial Research Organization, Melbourne, pp. 225-235.
C. S. Kenneth and E. G. William, “A Root-Injection Method to Assess Verticillium Wilt Resistance of Peppermint (Menth x piperita L.) and Its Use in Identifying Resistant Somaclones of cv. Black Mitcham,” Euphytica, Vol. 106, No. 3, 1999, pp. 223-230.
F. Daayf, M. Nicole, B. Boher, A. Pando and J. P. Geiger, “Early Vascular Defense Reactions of Cotton Roots Infected with a Defoliating Mutant Strain of Verticillium dahliae,” European Journal of Plant Pathology, Vol. 103, No. 2, 1997, pp. 125-136. doi:10.1023/A:1008620410471
N. A. Garas and A. C. J. Waiss, “Differential AccumuLation and Distribution of Antifungal Sesquiterpenoids in Cotton Stems Inoculated with Verticillium dahliae,” Phytopathol, Vol. 76, 1996, pp. 1011-1017. doi:10.1094/Phyto-76-1011
N. A. Garas, S. Wilhem and J. E. Sagen, “Relationship of Cultivar Resistance to Distribution of Verticillium dahliae in Inoculated Cotton Plants and to Growth of Single Condia on Excised Stem Segments,” Phytopathol, Vol. 76, No. 10, 1986, pp. 1005-1010. doi:10.1094/Phyto-76-1005
M. E. Mace, R. D. Stipanoyic and A. A. Bell, “Toxicity and Role of Terpenoid Phytoalexins in Verticillium wilt Re-Sistance in Cotton,” Physiological Plant Pathology, Vol. 26, No. 2, 1985, pp. 209-218. doi:10.1016/0048-4059(85)90021-9
M. J. R. Mould and M. C. Heath, “Ultra-Structural Evidence of Differential Changes in Transcription, Translation, and Cortical Microtubules during in Planta Penetration of Cells Resistant or Susceptible to Rust Infection,” Physiological and Molecular Plant Pathology, Vol. 55, No. 4, 1999, pp. 225-236. doi:10.1006/pmpp.1999.0224
W. C. Muller and A. T. Morgham, “Ultra-Structure of the Vascular Responses of Cotton to Verticillium dahlia,” Canadian Journal of Botany, Vol. 71, No. 1, 1993, pp. 3236.
I. A. Dubery and V. Slater, “Induced Defence Responses in Cotton Leaf Disks by Elicitors from Verticillium dahliae,” Phytochem, Vol. 44, No. 8, 1997, pp. 1429-1434. doi:10.1016/S0031-9422(96)00635-8
R. Rakwal, S. Katsuhiko, K. A. Ganesh and Y. Masami, “Protein Phosphatase Inhibitors Activate Defense Responses in Rice (Oryza sativa) Leaves,” Physiologia Plantarum, Vol. 111, No. 2, 2001, pp. 151-157. doi:10.1034/j.1399-3054.2001.1110204.x
S. R. McLusky, M. H. Bennett, M. H. Beale, M. J. Lewis, P. Gaskin and J. W. Mansfield, “Cell Wall Alterations and Localized Accumulation of Feruloyl-3’-methoxytyamine in Onion Epidermis at Sites of Attempted Penetration by Botrytis allii Are Associated with Actin Polarision, Peroxidase Activity and Suppression of Flavonoid Biosynthesis,” The Plant Journal, Vol. 17, No. 5, 1999, pp. 523-534. doi:10.1046/j.1365-313X.1999.00403.x
R. Meyer and I. A. Dubery, “High-Affinity Binding of a Protein-Lipopolysaccharide Phytotoxin from Verticillium dahliae to Cotton Membranes,” FEBS Letters, Vol. 335, No. 2, 1993, pp. 203-206. doi:10.1016/0014-5793(93)80730-I
S.-C. C. Lo, J. D. Hipskind and R. L. Nicholson, “cDNA Cloning of a Sorghum Pathogenesis-Related Protein (PR10) and Differential Expression of Defense-Related Genes Following Inoculation with Cochliobolus heterostropohus or Colletotrichum sublineolum,” Molecular Plant-Microbe Interactions, Vol. 12, No. 6, 1999, pp. 479-489. doi:10.1094/MPMI.1999.12.6.479
L. F. Li, “Multilayer-Coated Diffraction Gratings: Differential Method of Chandezon et al. Revisited: Errata,” Journal of the Optical Society of American A, Vol. 13, No. 3, 1996, p. 534. doi:10.1364/JOSAA.13.000543
D. J. Bowels, “Defense-Related Proteins in Higher Plants,” Annual Review of Biochemistry, Vol. 59, 1990, pp. 87-89.
L. C. Van Loon, W. S. Pierpoint, T. Boller and V. Conejero, “Recommendation for Naming Plant PathogenesisRelated Proteins,” Plant Molecular Biology Reporter, Vol. 12, No. 3, 1994, pp. 245-264. doi:10.1007/BF02668748
M. H. Walter, J. W. Liu, J. Wunn and D. Hess, “Bean Ribonuclease-Like Pathogenesis-Related Protein Genes (Ypr10) Display Complex Patterns of Developmental, Dark-Induced and Exogenous-Stimulus Dependent Expression,” European Journal of Biochemistry, Vol. 239, No. 2, 1996, pp. 281-293. doi:10.1111/j.1432-1033.1996.0281u.x
N. S. Jwa, G. K. Agrawal, R. Rakwal, C. H. Park and V. S. Agrawal, “Molecular Cloning and Characterization of a Novel Jasmonate Inducible Pathogenesis-Related Class 10 Protein Gene, JISsPR10, from Rice (Oryza sativa L.) Seedling Leaves,” Biochemical and Biophysical Research Communications, Vol. 76, 1996, pp. 1011-1017.
M. Hashimoto, L. Kisseleva, S. Sawa, T. Furukawa, S. Komatsu and T. Koshiba, “A Novel Rice PR10 Protein, RSOsPR10, Specifically Induced in Roots by Biotic and Abiotic Stresses, Possibly via the Jasmonic Acid Signaling Pathway,” Plant Cell Physiology, Vol. 45, No. 5, 2004, pp. 550-559. doi:10.1093/pcp/pch063
F. Smit and I. A. Dubery, “Cell Wall Reinforcement in Cotton Hypocotyls in Response to Verticillium dahlia Elicitor,” Phytochemistry, Vol. 44, No. 5, 1997, pp. 811815. doi:10.1016/S0031-9422(96)00595-X
K. J. Zuo, J. Wang, W. Wu, Y. Chai, X. Sun and K. X. Tang, “Identification and Characterization of Differentially Expressed ESTs of Gossypium barbadense Infected by Verticillium dahliae with Suppression Subtractive Hybridization,” Molecular Biology, Vol. 39, No, 2, 2005, pp. 191-191. doi:10.1007/s11008-005-0028-6
K. H. Melissa, J. L. Karin and R. L. Bruce, “Identification of Disease Response Genes Expressed in Gossypium hirsutum,” Plant Molecular Biology, Vol. 40, No. 2, 1999, pp. 286-296.
H. G. McFadden, R. Chapple, R. de Feyter and E. Dennis, “Expression of Pathogenesis-Related Genes in Cotton Stems in Response to Infection by Verticillium dahlia,” Physiological and Molecular Plant Pathology, Vol. 58, No. 3, 2001, pp. 119-131.
J. Sambrook, E. F. Fritsch and T. Maniatis, “Molecular Cloning, a Laboratory Manual,” 2nd Edition, Cold Spring Harbor laboratory Press, New York, 1989.
Y. Tateno, N. Takezaki and M. Nei, “Relative Efficiencies of the Maximum-Likelihood, Neighbor-Joining, and Maximum-Parsimony Methods When Substitution Rate Varies with Site,” Molecular Biology and Evolution, Vol. 11, No. 2, 1994, pp. 261-277.
K. Tamura, J. Dudley, M. Nei and S. Kumar, “MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0,” Molecular Biology and Evolution, Vol. 24, No. 8, 2007, pp. 1596-1599. doi:10.1093/molbev/msm092
M. Gajhede, P. Osmark, F. E. Poulsen, H. Ipsen, J. N. Larsen, R. J. J. van Neerven, C. Schou, H. L?wenstein and M. D. Spangfor, “X-Ray and NMR Structure of Betv1, the Origin of Birch Pollen Allergy,” Nature Structural & Molecular Biology, Vol. 3, No. 12, 1996, pp. 10401045. doi:10.1038/nsb1296-1040
M. Saraste, P. R. Shibbald and A. Wittinghofer, “The PLoop—A Common Motif in ATPand GTP-Binding Proteins,” Trends in Biochemical Sciences, Vol. 15, No. 11, 1990, pp. 430-434. doi:10.1016/0968-0004(90)90281-F
X. J. Zhou, S. Lu, Y. H. Xu, J. W. Wang and X. Y. Chen, “A Cotton cDNA (GaPR10) Encoding a PathogenesisRelated 10 Protein with in Vitro Ribonuclease Activity,” Plant Science, Vol. 162, No. 4, 2002, pp. 629-636. doi:10.1016/S0168-9452(02)00002-X
Y. H. Cheong, H. Chang, R. Gupta, X. Wang, T. Zhu and S. Luan, “Transcriptional Profiling Reveals Novel Interactions between Wounding, Pathogen, Abiotic Stress and Hormonal Responses in Arabidopsis,” Plant Physiology, Vol. 129, No. 2, 2002, pp. 661-677. doi:10.1104/pp.002857
M. E. Mace, A. A. Bell and C. H. Beckman, “Histochemistry and Identification of Disease-Induced Terpenoid Aldehydes in Verticillium-Wilt-Resistant and -Susceptible Cottons,” Canadian Journal of Botany, Vol. 54, No. 18, 1976, pp. 2095-2099. doi:10.1139/b76-225
P. Constabel and C. A. Ryan, “A Survey of Woundand Methyl Jasmonate-Induced Leaf Polyphenol Oxidase in Crop Plants,” Phytochemistry, Vol. 47, No. 4, 1998, pp. 507-511. doi:10.1016/S0031-9422(97)00539-6
I. A. Dubery, L. G. Teodorczuk and A. E. Louw, “Early Responses in Methyl-Jasmonate Preconditioned Cells toward Pathogen-Derived Elicitors,” Molecular Cell Biology Research Communications, Vol. 3, No. 2, 2000, pp. 105-110. doi:10.1006/mcbr.2000.0198