<i>Pinus taeda</i> cDNA Microarray as a Tool for Candidate Gene Identification for Local Red/Far-Red Light Adaptive Response in <i>Pinus sylvestris</i> — Oak Academic Publishing
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<i>Pinus taeda</i> cDNA Microarray as a Tool for Candidate Gene Identification for Local Red/Far-Red Light Adaptive Response in <i>Pinus sylvestris</i>
Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
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Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
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Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
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Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
1 Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
2 Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
3 Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
4 Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umea, Sweden.
Light quality response is a vital environmental cue regulating plant development. Conifers, like angiosperms, respond to the changes in light quality including the level of red (R) and far-red (FR) light, which follows a latitudinal cline. R and FR wavelengths form a significant component of the entire plant life cycle, including the initial developmental stages such as seed germination, cotyledon expansion and hypocotyl elongation. With an aim to identify differentially ex pressed candidate genes, which would provide a clue regarding genes involved in the local adaptive response in Scots pine ( Pinus sylvestris ) with reference to red/far-red light; we performed a global expression analysis of Scots pine hy pocotyls grown under two light treatments, continuous R (cR) and continuous FR (cFR) light; using Pinus taeda cDNA microarrays on bulked hypocotyl tissues from different individuals, which represented different genotypes. This ex periment was performed with the seeds collected from northern part of Sweden (Ylinen, 68?N). Interestingly, gene ex pression pattern with reference to cryptochrome1, a blue light photoreceptor, was relatively high under cFR as com pared to cR light treatment. Additionally, the microarray data analysis also revealed expression of 405 genes which was enhanced under cR light treatment; while the expression of 239 genes was enhanced under the cFR light treatment. Dif ferentially expressed genes were re-annotated using Blast2GO tool. These results indicated that cR light acts as pro moting factor whereas cFR antagonises the effect in most of the processes like C/N metabolism, photosynthesis and cell wall metabolism which is in accordance with former findings in Arabidopsis . We propose cryptochrome1 as a strong candidate gene to study the adaptive cline response under R and FR light in Scots pine as it shows a differential expres sion under the two light conditions.
KeywordsConiferCryptochrome1Far-Red LightMicroarrayRed LightScots Pine
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