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Construction and Analysis of SSH-cDNA Library from Leaves of Susceptible Rubber Clone Resistant to Powdery Mildew Induced by BTH
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 1 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 3 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 4 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 5 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 6 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
- 7 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture/Key Laboratory of Pests Detection and Control for Tropical Agriculture, Haikou, China.
American Journal of Plant Sciences·Volume 04 (2013)·Pages 528–534·Published 14 March 2013·DOI10.4236/ajps.2013.43068
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Abstract
To understand the mechanism of benzothiadiazole (BTH)-induced susceptible rubber clone resistance to powdery mildew on gene level, a differentially expressed cDNA library was constructed by suppression subtractive hybridization (SSH) with rubber Reyan 7-33-97 clone. The constructed cDNA library was high integrity through detection of the critical processes of SSH, such as efficiency of adaptor connection, subtraction and conversion, as well as the type of recombinant genes. The positive rate was 99% after identification with random 400 white spots. The size of the cDNA clone inserted fragments was various but most in 400 bp - 1000 bp. There were 23 cDNA sequences matching the function of energy and basic metabolism, signal transduction, membrane and transport, secondary metabolism and so on after detection of the 42 positive clone sequences selected randomly from the cDNA library and comparison on nucleic acid sequences in Genbank. 7 ESTs were logged in Genbank and accession numbers were GW873071 and GW874604- GW874610. The results implicated that BTH could effectively induced rubber tree resistance to powdery mildew through increasing expresses of defense-related genes in leaves of rubber tree susceptible clone. It should provide a new approach for rubber disease management.
KeywordsBenzothiadiazoleESTsHevea brasiliensisInduced ResistanceOidium heveaeSuppression Subtractive Hybridization
- G. C. Mondal and K. Jacob, “Effect of Powdery Mildew Disease on Yield of Rubber in Northern Part of West Bengal,” Proceedings of Placrosym, 2002, pp. 531-534.
- J. Liu, “Recent Advances in Rubber Powdery Mildew Research,” Tropical Agricultural Science & Technology, Vol. 33, No. 3, 2010, pp. 1-5.
- J. L. Shan, Q. C. Xiao, Z. T. Yu, et al., “A Preliminary Study on Mechanism of Oligosaccharin Inducing Rubber Tree Resistance to Powdery Mildew,” Subtropical Plant Science, Vol. 34, No. 1, 2005, pp. 31-32. doi:10.1086/425207
- C. J. Luo, Z. W. Fan, Y. D. Shen, H. T. Cheng and L. Z. Liu, “Effects of Rubber Tree Resistance Induced by BTH to Oidium heveae and Assay of Resistance-Related Enzymes,” Chinese Journal of Tropical Crop, Vol. 32, No. 3, 2011, pp. 475-479.
- Z. Sun and F. C. Zheng, “Effect of BTH on Resistance Induction in Rubber against Colletotrichum gloeosporioides Disease,” Guangdong Agricultural Science, No. 7, 2008, pp. 76-77.
- J. Goerlach, S. Volrath, G. Knauf-Beiter, et al., “Benzothiadiazole, a Nevel Class of Inducers of Systemic Acquired Resistance, Activates Genes Expression and Disease Resistance in Wheat,” The Plant Cell, Vol. 8, 1996, pp. 629-643.
- C. Nombela, S. Pascual, M. Aviles, et al., “Benzothiadiazole Induces Local Resistance to Bemisia tabaci (Hemiptera: Aleyrodidae) in Tomato Plants,” Journal of Economic Entomology, Vol. 98, No. 6, 2005, pp. 2266-2271. doi:10.1603/0022-0493-98.6.2266
- B. Chinnasri, B. S. Sipes and D. P. Schmitt, “Effects of Acibenzolar-S-Methyl Application to Rotylenchulus reniformis and Meloidogyne javanica,” Journal of Nematology, Vol. 35, No. 1, 2003, pp. 110-114.
- Z. W. Fan, H. Buschmann and J. Sauerborn, “Main Effects and Interactions among Acibenzolar-S-Methyl, a Biocontrol Fungus and Sunflower Cultivar on Control of Orobanche cumana Wallr.,” Journal of Plant Diseases and Protection, Vol. 114, No. 2, 2007, pp. 76-81.
- L. Z. Xiong, M. W. Lee, M. Qi and Y. N. Yang, “Identification of Defense-Related Rice Genes by Suppression Subtractive Hybridization and Differential Screening,” Molecular Plant-Microbe Interaction, Vol. 14, No. 5, 2001, pp. 685-692. doi:10.1094/MPMI.2001.14.5.685
- K.-H. Kogel and G. Langen, “Induced Disease Resistance and Gene Expression in Cereals,” Cellular Microbiology, Vol. 7, No. 11, 2005, pp. 1555-1564. doi:10.1111/j.1462-5822.2005.00592.x
- C. Bovie, M. Ongena, P. Thonart, et al., “Cloning and Expression Analysis of cDNAs Corresponding to Genes Activated in Cucumber Showing Systemic Acquired Resistance after BTH Treatment,” BMC Plant Biology, Vol. 4, 2004, pp. 15-26. doi:10.1186/1471-2229-4-15