AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i> — Oak Academic Publishing
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AACA Element Negatively Regulates Expression of Protein Phosphatase 2C (<i>PP2C</i>) like Promoter in <i>Arabidopsis thaliana</i>
Department of Biological Sciences, Birla Institute of Technology & Sciences (BITS), Pilani, India
,
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Government of India, Mohali, India
,
Department of Biological Sciences, Birla Institute of Technology & Sciences (BITS), Pilani, India.
,
Department of Biological Sciences, Birla Institute of Technology & Sciences (BITS), Pilani, India.
1 Department of Biological Sciences, Birla Institute of Technology & Sciences (BITS), Pilani, India
2 National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Government of India, Mohali, India
3 Department of Biological Sciences, Birla Institute of Technology & Sciences (BITS), Pilani, India.
4 Department of Biological Sciences, Birla Institute of Technology & Sciences (BITS), Pilani, India.
Genome wide analysis of Arabidopsis thaliana reveals a unique genetic arrangement of ACGT motif in the protein phosphatase 2C ( PP 2 C ) like promoter (accession number AT 5G 59220). In the present study, full length, 900 base pair, 500 base pair, 400 base pair and NRM deletion variants of PP 2 C like promoter were constructed to investigate the activity of PP 2 C like promoter in the presence of inducers like abscisic acid, jasmonic acid and salicylic acid. The PP 2 C promoter has three ACGT elements in close vicinity. They are so positioned that they are separated by a spacer of 30 base pair and 5 base pair respectively from each other. The study has shown that ACGT N30 ACGT genetic architecture is essential for the promoter to be induced in response to abscisic acid. The synergistic and antagonistic effects of cis elements were observed. AACA is a positive regulatory element in endosperm and is known to act as negative regulatory element in other tissues. In this study AACA, have been found to negatively regulate the expression of reporter gene EGFP in both induced and under uninduced conditions.
KeywordsAbscisic AcidACGTAACAAbiotic Stress
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