<i>In Silico</i> Analysis of a MRP Transporter Gene Reveals Its Possible Role in Anthocyanins or Flavonoids Transport in <i>Oryze sativa</i>
- 1 College of Life Science, Genetic Engineering Laboratory, South China Agricultural University, Guangzhou, China.
- 2 College of Life Science, Genetic Engineering Laboratory, South China Agricultural University, Guangzhou, China.
- 3 College of Life Science, Genetic Engineering Laboratory, South China Agricultural University, Guangzhou, China.
- 4 College of Life Science, Genetic Engineering Laboratory, South China Agricultural University, Guangzhou, China.
- 5 College of Forestry, South China Agricultural University, Guangzhou, China.
- 6 Vegetable Research Institute, Guangdong Academy of Agriculture Science, Guangzhou, China.
Abstract
There are many studies on enzymatic pathways of anthocyanin biosynthesis, but little is known about the anthocyanins transport in Oryze sativa . In silico analysis, the OsMRP 15 (LOC_Os 06g 06440), an orthologous gene of mazie anthocyanin transporter ZmMRP 3, has been identified in rice. The OsMRP 15 contained a 4425bp open reading frame (ORF) encoding a 1475 amino acid protein, belonging to a MRP subfamily of ABC transporters, and has a high sequence identity, very similar protein structure, and the same arrangement of domains to ZmMRP 3, but the genomic structure of OsMRP 15 was significant difference with ZmMRP 3. The prediction promoter of OsMRP 15 has many presumed anthocyanin regulatory sites. The phylogenetic analysis of MRPs in rice, mazie and Arabidopsis showed that OsMRP 15 and ZmMRP 3 belonged to the same subbranch. The expression pattern indicated that OsMRP 15 was co-expression with two anthocya nin transcription factors. These analysis results implied that as an ortholog of ZmMRP 3, the function of OsMRP 15 was possibly as a membrane-bound transporter required for vacuolar uptake of anthocyanins in rice .
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