Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds — Oak Academic Publishing
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Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds
Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
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Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
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Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
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Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
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Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
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Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
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Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
1 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
2 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
3 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
4 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
5 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
6 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
7 Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Peanut ( Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR 10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the ex pression of ARAhPR 10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype “ GT-C 20” and susceptible genotype “ Yueyou 7” . Seven transgenic peanut lines expressing the ARAhPR 10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens -mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR 10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR 10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR 10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.
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